Attractene Transfection Reagent
効果的なDNA トランスフェクションおよびDNA とsiRNA/miRNA のコトランスフェクション
- 非常に低い細胞毒性で効率的なDNAトランスフェクション
- 迅速なトランスフェクション用プロトコールにも対応可能
- 全付着細胞や感受性の高い細胞にも適した汎用的な試薬
- コトランスフェクションやベクター利用のRNAi に最適
- 動物由来の成分フリー
次世代の脂質テクノロジーを利用したAttractene Transfection Reagent は、真核細胞へ効率の高いDNA トランスフェクションを実現します。非リポソーム系脂質試薬のAttractene Reagent は、HaCaT、MonoMac6、HCT116 などのトランスフェクトが困難な細胞を含む全ての付着細胞やいくつかの浮遊細胞(Jurkat, K562)へ効率的にトランスフェクトできます。またDNAとsiRNA/miRNA mimics/Inhibitorsとのコトランスフェクションにも最適です。
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Attractene Transfection Reagent (1 ml)
Attractene Transfection Reagent for up to 660 transfections in 24-well plates
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301005
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Attractene Transfection Reagent (4 x 1 ml)
Attractene Transfection Reagent for up to 2640 transfections in 24-well plates
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301007
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The Attractene Transfection Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Attractene Reagent outperforms alternative reagents.|Healthy cells after transfection using Attractene Reagent.|Flexible, rapid Fast-Forward Protocols.|Effective knockdown after shRNA vector transfection.|
DNA (pGFP which expresses green fluorescent protein) was transfected into the cell types indicated using Attractene Reagent according to the conditions recommended in the handbook. Transfection was also performed with [A] Reagent F using 2 reagent volumes recommended by the manufacturer or [B] Reagent L according to the manufacturer's recommendations, with and without the enhancer provided. Transfection efficiency was estimated by counting the number of fluorescent cells by FACS analysis. Transfection efficiency is expressed relative to efficiency using Attractene Reagent which was set at 100%.|HepG2 cells were transfected with DNA (pGFP) using Attractene Transfection Reagent or Reagent L from another supplier according to the manufacturer's instructions. FACS analysis confirmed equal numbers of transfected cells in both cultures. Two days after transfection, cells were examined by light microscopy. [A] Cells transfected using Attractene Reagent were healthy and viable. [B] In contrast, cells transfected using Reagent L displayed high levels of cell death. [C] Cell viability was measured using a CellTiter-Blue assay (Promega). Viability was significantly lower in cells transfected using Reagent L compared to cells transfected using Attractene Reagent (set at 100%).|Traditionally, cells are seeded the day before transfection. Using the Fast-Forward Protocol, seeding and transfection take place on the same day. This saves time and effort.|HEK293 cells were [A] transfected with a plasmid expressing green fluorescent protein (pGFP) only, or [B] cotransfected with pGFP and a plasmid expressing an shRNA targeted against the green fluorescent protein gene (pGFP + pshGFP), or [C] cotransfected with pGFP and a negative control plasmid expressing a scrambled shRNA (pGFP + pNegative). After cotransfection of pGFP and the shRNA vector pshGFP, the green fluorescent protein was effectively silenced indicating effective cotransfection.|
Principle
Attractene Reagent は非リポゾーム系脂質試薬であり、いくつかの浮遊細胞(Jurkat、 K562)や、HaCaT、MonoMac6、HCT116 などのトランスフェクトが困難な細胞を含む付着細胞へのDNAトランスフェクションを効率的に行ないます。Attractene Reagent は、幅広い実験条件下で、他の試薬と比較よりも常に高いトランスフェクション効率を発揮します。この特性により、 自動化プラットフォーム使用やハイスループットおよびロースループットのトランスフェクションなどの多くの実験のセットアップに柔軟な操作性と簡便性を実現します。またDNAとsiRNA/miRNA mimics/Inhibitorsとのコトランスフェクションにも最適です。Attractene Reagentは、細胞毒性が極めて低く、トランスフェクション実験成功に非常に重要なポイントです。
Procedure
Attractene Transfection Reagent は、迅速なDNAトランスフェクションに最適です。迅速なトランスフェクション用プロトコールでは、細胞播種とトランスフェクションを同じ日に行ないます(図 "柔軟な迅速トランスフェクション用プロトコール")。トランスフェクション前日に細胞を播種するプロトコールに比べて、本プロトコールは時間と労力を削減でき、実験の柔軟性を向上します。 TransFect Protocol Databaseで細胞特異的なプロトコールを提供 Attractene Transfection Reagent Handbookに掲載されているプロトコールだけではなく、お客様の細胞種やプレート/ディッシュフォーマットに最適なプロトコールをTransFect Protocol Databaseで見つけられます。このデータベースから必要なプロトコールを的確に入手でき、時間と労力を節約できます。使用する細胞や核酸のタイプを入力するだけで、QIAGENトランスフェクションプロトコールを印刷あるいは便利なPDFフォーマットとしてダウンロードできます。TransFect Protocol Databaseの使用は無料で、登録は不要です。
Applications
Attractene Transfection Reagentは、トランジェントあるいはステーブルなトランスフェクション、異なるDNA分子のコトランスフェクション、DNAとsiRNA、あるいはmiRNA mimicまたはinhibitorsとのコトランスフェクションなどの幅広いトランスフェクションのアプリケーションに適しています。Attractene Transfection Reagentは以下のようなアプリケーションに最適です。 機能ゲノム学 ハイスループットDNAトランスフェクション 遺伝子サイレンシング
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Feature
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Specifications
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Applications
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Plasmid transfection, shRNA vector transfection, protein overexpression, reporter studies, RNAi experiments
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Cell type
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All cell types including sensitive and difficult to transfect cells
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Controls
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Not included
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Features
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Highly efficient transfection with lowest toxicity. Rapid fast forward transfection protocol. Free of animal-derived components. Transfection in the presence of serum.
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Nucleic acid
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DNA
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Number of possible transfections
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up to 660 transfections in 24-well plates / 1 ml reagent
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Technology
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Cationic lipid based transfection reagent
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Transfection type
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Transient transfection, stable transfection, plasmid co-transfection
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Brochure detailing reagents for efficient and robust DNA and RNA transfection.
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View
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For efficient DNA transfection of a broad range of cell lines, including sensitive cell types
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Images
Attractene Reagent outperforms alternative reagents.
DNA (pGFP which expresses green fluorescent protein) was transfected into the cell types indicated using Attractene Reagent according to the conditions recommended in the handbook. Transfection was also performed with [A] Reagent F using 2 reagent volumes recommended by the manufacturer or [B] Reagent L according to the manufacturer's recommendations, with and without the enhancer provided. Transfection efficiency was estimated by counting the number of fluorescent cells by FACS analysis. Transfection efficiency is expressed relative to efficiency using Attractene Reagent which was set at 100%.
Healthy cells after transfection using Attractene Reagent.
HepG2 cells were transfected with DNA (pGFP) using Attractene Transfection Reagent or Reagent L from another supplier according to the manufacturer's instructions. FACS analysis confirmed equal numbers of transfected cells in both cultures. Two days after transfection, cells were examined by light microscopy. [A] Cells transfected using Attractene Reagent were healthy and viable. [B] In contrast, cells transfected using Reagent L displayed high levels of cell death. [C] Cell viability was measured using a CellTiter-Blue assay (Promega). Viability was significantly lower in cells transfected using Reagent L compared to cells transfected using Attractene Reagent (set at 100%).
Flexible, rapid Fast-Forward Protocols.
Traditionally, cells are seeded the day before transfection. Using the Fast-Forward Protocol, seeding and transfection take place on the same day. This saves time and effort.
Effective knockdown after shRNA vector transfection.
HEK293 cells were [A] transfected with a plasmid expressing green fluorescent protein (pGFP) only, or [B] cotransfected with pGFP and a plasmid expressing an shRNA targeted against the green fluorescent protein gene (pGFP + pshGFP), or [C] cotransfected with pGFP and a negative control plasmid expressing a scrambled shRNA (pGFP + pNegative). After cotransfection of pGFP and the shRNA vector pshGFP, the green fluorescent protein was effectively silenced indicating effective cotransfection.
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