QIAcuity Digital PCR System
ナノプレートベースのデジタルPCR
- 統合型のデジタルPCR システム
- 必要な処理数に合わせた製品バリエーション（１，４そして8プレート処理）
- 一歩進んだマルチプレックス性能（最大5プレックス）
- 柔軟性に富んだサンプルスループット
- 結果までわずか2時間
QIAcuity デジタルPC Rシステムは、変異検出、コピー数多型（CNV）、遺伝子発現研究、遺伝子編集分析などで正確な定量結果を提供するように設計されています。このナノプレートベースのシステムは、サンプルの分配、PCR 増幅そしてイメージングの標準的なdPCR ワークフローを、わずかな時間で全自動処理することができます。
このシステムでは専用の QIAcuity ナノプレートおよびアクセサリーを使用します。
QIAcuityについての詳しい情報は、バーチャルデモにお進みください。
en-CA
|
Product
|
Cat. no.
|
List price:
|
|
|
QIAcuity One, 2plex Device
One-plate digital PCR instrument for detecting up to 2 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.
|
911001
|
|
|
|
QIAcuity One, 2plex System
One-plate digital PCR instrument for detecting up to 2 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.
|
911002
|
|
|
|
QIAcuity One, 2plex System FUL-2
One-plate digital PCR instrument for detecting up to 2 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.
|
911006
|
|
|
|
QIAcuity One, 5plex Device
One-plate digital PCR instrument for detecting up to 5 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.
|
911021
|
|
|
|
QIAcuity One, 5plex System
One-plate digital PCR instrument for detecting up to 5 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.
|
911022
|
|
|
|
QIAcuity One, 5plex System FUL-2
One-plate digital PCR instrument for detecting up to 5 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.
|
911026
|
|
|
|
QIAcuity Four Platform System
Four-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit.1 year warranty on labor, travel, and parts.
|
911042
|
|
|
|
QIAcuity Four Platform System FUL-2
Four-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.
|
911046
|
|
|
|
QIAcuity Four Platform System PRM-3
Four-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Premium Agreement for 3 years with a next business day response time, and 3 preventive maintenance visits.
|
911049
|
|
|
|
QIAcuity Eight Platform System
Eight-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, nanoplate roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts.
|
911052
|
|
|
|
QIAcuity Eight Platform System FUL-2
Eight-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, nanoplate roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.
|
911056
|
|
|
|
QIAcuity Eight Platform System PRM-3
Eight-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, nanoplate roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Premium Agreement for 3 years with a next business day response time, and 3 preventive maintenance visits.
|
911059
|
|
|
|
QIAcuity One Installation & Training
Installation of instrument hardware and system software. Includes application training for hands-on experience in operating the instrument and qualifying laboratory staff. Labor, travel, and installation kit (if applicable) included.
|
9245352
|
|
|
|
QIAcuity Four Installation & Training
Installation of instrument hardware and system software. Includes application training for hands-on experience in operating the instrument and qualifying laboratory staff. Labor, travel, and installation kit (if applicable) included.
|
9245353
|
|
|
|
QIAcuity Eight Installation & Training
This service combines Installation with Applications Training to help customers gain hands-on experience of hardware, software, applications using QIAGEN platforms and facilitates professional qualifications of laboratory staff.
|
9245354
|
|
|
|
QIAcuity One Preventive Subscription
One on-site preventive maintenance (PM), including travel, labor, and PM parts. Includes a 10% discount on repair services during Preventive Subscription period and documentation in a GMP/GLP-compliant visit report.
|
9245355
|
|
|
|
QIAcuity Four Preventive Subscription
One on-site preventive maintenance (PM), including travel, labor, and PM parts. Includes a 10% discount on repair services during Preventive Subscription period and documentation in a GMP/GLP-compliant visit report.
|
9245356
|
|
|
|
QIAcuity Eight Preventive Subscription
One on-site preventive maintenance (PM), including travel, labor, and PM parts. Includes a 10% discount on repair services during Preventive Subscription period and documentation in a GMP/GLP-compliant visit report.
|
9245357
|
|
|
|
QIAcuity One 2plex Core Agreement
One on-site repair service and one-on-site preventive maintenance including travel, labor, and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during agreement period.
|
9245358
|
|
|
|
QIAcuity One 5plex Core Agreement
One on-site repair service and one-on-site preventive maintenance including travel, labor, and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during agreement period.
|
9245359
|
|
|
|
QIAcuity Four Core Agreement
One on-site repair service and one-on-site preventive maintenance including travel, labor, and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during agreement period.
|
9245360
|
|
|
|
QIAcuity Eight Core Agreement
One on-site repair service and one-on-site preventive maintenance including travel, labor, and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during agreement period.
|
9245361
|
|
|
|
QIAcuity One 2plex Full Agreement
On-site QIAcuity One 2plex instrument repair, including travel, labor, and parts, for a period of one year. Response time of two business days. Includes one preventive maintenance during the Full Agreement period.
|
9245362
|
|
|
|
QIAcuity One 5plex Full Agreement
On-site QIAcuity One 5plex instrument repair, including travel, labor, and parts, for a period of one year. Response time of two business days. Includes one preventive maintenance during the Full Agreement period.
|
9245363
|
|
|
|
QIAcuity Four Full Agreement
On-site QIAcuity Four instrument repair, including travel, labor, and parts, for a period of one year. Response time of two business days. Includes one preventive maintenance during the Full Agreement period.
|
9245364
|
|
|
|
QIAcuity Eight Full Agreement
On-site QIAcuity Eight instrument repair, including travel, labor, and parts, for a period of one year. Response time of two business days. Includes one preventive maintenance during the Full Agreement period.
|
9245365
|
|
|
QIAcuity は分子生物学のアプリケーションにおいての使用を前提としております。この製品は、診断、病気の予防または治療を目的とした使用は想定しておりません。そのため臨床利用（診断、予後、治療または血液バンク）のための製品の性能特性は不明です。
Performance
QIAcuity デジタルPCR システムは、全てのラボへ絶対定量をお手頃な価格で提供します。サンプルの分配、PCR 増幅、イメージングのデジタルPCR ワークフロー全体を、全自動設計のQIAcuity は無駄な時間をかけずに処理します。また、現在のqPCR アッセイをQIAcuity デジタルPCRシステムに適合させることも簡単です。qPCR の場合、プレート操作を変える必要はなく、迅速なアッセイセットアップと2時間以内の迅速な結果提供をお約束します。
QIAcuity – 性能と仕様
| Feature |
QIAcuity One |
QIAcuity Four |
QIAcuity Eight |
| プレート処理数 |
1 |
4 |
8 |
| 検出チャンネル |
2 または 5 |
5 |
5 |
| PCR装置 |
1 |
1 |
2 |
| 結果までの時間 |
2時間以内 |
最初のプレートは2時間以内
その後のプレートは1枚当たり1時間以内
|
最初のプレートは2時間以内
その後のプレートは1枚当たり1時間以内
|
| 1日のサンプル処理数 |
最大384（96ウェル）
最大96（24ウェル）
|
最大672（96ウェル）
最大168（24ウェル）
|
最大1248（96ウェル）
最大312（24ウェル）
|
Principle
サンプルロード、測定そして結果解析という、わずか3つのステップで、2時間以内にdPCRの結果が得られます。
ナノプレートでのｄPCR反応の原理はこちら。
Procedure
qPCR 実験のようにサンプル調整には、マスターミックス、プローブ、プライマーを96または24ウェルナノプレートへ加え、その後サンプルを加えるステップは存在しますが、このシステムでは、サンプルの分配、PCR増幅およびイメージングを1台の装置の中で自動化し、サンプルから結果までを2時間以内に完了します。ソフトウェアは、ターゲットシークエンスのマイクロリットルあたりのコピー数を計算するだけでなく、ポジティブサンプルやNTCなどのコントロールも同時に解析します。またこの解析は、同じローカルエリアネットワーク（LAN）内の離れた場所のPCでも行えます。
Applications
QIAcuity は、QIAcuity ナノプレートおよびQIAcuity PCR キットとともに、以下を含むデジタルPCR アプリケーションに利用可能です。
- 希少な突然変異の検出
- コピー数多型解析
- 遺伝子発現解析
- 病原菌の検出
- ジェノタイピング
- miRNA 研究
Services
QIAGEN の多様なサービスソリューションなら機器を安心してご利用いただけます。ニーズに合ったサービス契約商品の詳細は弊社また弊社契約代理店にお尋ねください。
Software
QIAcuity ソフトウェアは、装置に直接または既存のローカルエリアネットワーク（LAN）を使用して、1台または複数のQIAcuity 装置を制御することができます。 QIAcuity ソフトウェアを使用して、デジタルPCR 実験、サンプルや反応液を定義し、ナノプレートに割り当て、そしてQIAcuity 機器に情報を転送できます。装置の運転終了後は、データ解析とレポート作成をすることができます。さらに、データを次の解析システムへエクスポートすることができます。このソフトウェアは、繰り返し使うプレートレイアウトやパラメーターに簡単にアクセスできるようなテンプレート機能を持ち、デジタルPCRの実験をさらに柔軟に拡張させることができます。
ローカルエリアネットワークに載せれば、そのコンピューターはサーバーとしてQIAcuity ソフトウェアの機能をホストし、クライアントとして機能する他のコンピューターにLAN を経由してアクセスできるようになります。これにより、複数のユーザーが他の居室からソフトウェアにアクセスしたり、複数のコンピューターにソフトウェアをインストールしなくてもブラウザを介してデータの解析ができるようになります。
|
|
|
|
Version 1.1.3
|
Show details
|
|
|
Version 1.0
The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis.
This new version of the control software includes system stability and performance reliability improvements. We highly recommend to update the control software of your QIAcuity instrument.
Note: The installation requires approximately 90 minutes.
|
Show details
|
|
|
For Version 1.0
|
Show details
|
|
|
For Version 1.2
|
Show details
|
|
|
Version 1.2
The QIAcuity Software Suite 1.2 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).
The following browsers are supported in the QIAcuity Software Suite:
-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)
The new QIAcuity Software Suite 1.2 offers a functionality that enables users of the QIAcuity Software 1.1.3 to upgrade to the new version while keeping the library of previously stored plate runs.
Note: If you have exported plates from QIAcuity Software Suite 1.1.3 that you would like to import and use in QIAcuity Software Suite 1.2, you will need to import the plates before upgrading from version 1.1.3 to version 1.2. You may then export the plates again. Future software version starting from QIAcuity Software Suite 2.0 will facilitate import of plates from previous QIAcuity Software Suite versions.
The new improvements are as follows:
-Support for the Nanoplate 8.5k 24-well
-Hyperwell functionality to combine several wells to one combined well for analysis
-Automated plate archiving functionality
-Functionality to show the number of single/double positives in 2D scatterplots
-VPF (Volume Precision Factor) to further improve concentration calculation (see related resources)
-Additional improvements for stabilization and troubleshooting
|
Show details
|
|
|
Version 3.0
The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run.
In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.
After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.
Required QIAcuity Software Suite version: Version 1.2 or higher.
|
Show details
|
|
|
Version 2.0
The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis
Besides system stability and performance reliability improvements, this new version of the control software offers advanced user management and audit trail functionalities. Together with the QIAcuity Software Suite 2.0, the QIAcuity systems now support 21 CFR Part 11 regulations for users working under GMP.
Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.
Note: Upgrading the QIAcuity Control Software to version 2.0 will require upgrading the QIAcuity Software Suite to version 2.0.
Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.0. It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software!
|
Show details
|
|
|
For Version 2.0
|
Show details
|
|
|
For Version 2
|
Show details
|
|
|
Version 2.0
The QIAcuity Software Suite 2.0 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).
This new software version now supports 21 CFR Part 11 regulations for users working under GMP. This includes the following:
-Advanced User Management
-Audit Trail
-Signatures
Additional changes compared with the previous software version include the following:
-Usability improvements
-Improved dust detection
-Hyperwell expansion to CNV and GEX analysis
-CSV export functionality of multiple positive partitions for linkage analysis, drop-off assays, etc.
-Raw data export
Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.
Important: A direct update of the Software Suite from version 1.1.3 to 2.0.20 is not possible. Please follow the instructions in the User Manual on how to update to version 1.2.18 first before updating the Software Suite to version 2.0.20.
Before you start upgrading to version 1.1.3, please make sure that all plates are imported and visible in the plate overview of the QIAcuity Software Suite.
Caution: Not following the instructions may result in a loss of your previous plate data!
Please contact QIAGEN Technical Services if you are unsure and require technical support.
SHA1 checksum: C60C4EEC349DF1C037796016D32A93EEC19B54A0
|
Download
|
|
|
|
|
|
Show details
|
|
|
For use with QIAcuity systems
July 2021
|
Show details
|
|
|
User manual for QIAcuity instruments and QIAcuity Software 1.2
|
Show details
|
|
|
User manual for QIAcuity instruments and QIAcuity Software 2.0
|
Show details
|
|
|
Network connection of QIAcuity instruments
|
Show details
|
|
|
For QIAcuity Software Suite 1.2: Updating VPF for archived or exported plates
|
Show details
|
|
|
For QIAcuity User Manual – Updated QIAcuity Nanoplate Partition Volumes
|
Show details
|
|
|
|
|
Here we compared the performance of qPCR and the nanoplate dPCR techniques. The digital PCR method on the QIAcuity significantly improved precision when measuring copy number states and sensitivity of mutation detection through absolute quantification and reduced standard error. This is advantageous in various applications, including copy number variation analysis, small fold-change and rare mutation detection.
|
Show details
|
|
|
|
Show details
|
|