PyroMark Q96 Tests

PyroMark Q96 IDを用いた遺伝子特異的な配列変異あるいはメチル化の解析

がん関連遺伝子における変異の検出および定量
異なる遺伝子における個別あるいは複数のCpG部位を定量
目的領域での全体的なCpGメチル化を推定
前もってコントロール配列を組み込んで提供されるアッセイ

PyroMark Q96 Testsは遺伝子変異、SNP解析およびCpGメチル化の定量解析用のデザイン済みアッセイで、、PyroMark Q96IDでの使用に至適化されています。PyroMark HFEやPyroMark MTHFRのようなテストはPyroMark Q24でも利用できます（PyroMark APOEを除く）。

Ordering Information
Product Details
Product Resources

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Product	Cat. no.	List price:
PyroMark APOE (96) For detection of polymorphism in the APOE gene (2 x 96 reactions on PyroMark Q96 MD or 1 x 96 reactions on PyroMark Q96 ID)	972422	Log in
PyroMark HFE (96) For detection of variants in the HFE gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)	972442	Log in
PyroMark MTHFR (96) For detection of variants in the MTHFR gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)	972432	Log in
PyroMark Q96 CpG LINE-1 (2 x 96) For quantification of global methylation level in transposable elements; only for use with PyroMark Q96 MD	973043	4 495,00 kr	Add to cart
PyroMark Q96 CpG MGMT (96) For quantification of methylation level in the MGMT gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)	972032	4 495,00 kr	Add to cart
PyroMark Q96 CpG MLH1 (96) For quantification of methylation level in promoter region of the MLH1 gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)	972022	4 495,00 kr	Add to cart
PyroMark Q96 CpG p16 (96) For quantification of methylation level in the p16 gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)	972012	4 495,00 kr	Add to cart
PyroMark Q96 CpG Prader-Willi (96) For quantification of methylation level in Prader-Willi and Angelman Syndromes (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)	972052	4 495,00 kr	Add to cart

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PyroMark p16 analysis.|

[A] A normal blood DNA sample and [B] a DNA sample isolated from a colon tumor were analyzed. Blue columns indicate the measured CpG sites, and the individually measured degree of methylation at each site. Yellow columns indicate QC for completion of the bisulfite treatment.|

Performance

PyroMark Q96 Testsは PyroMark Q96 CpG p16 Testで例証されるように（図“p16遺伝子のPyroMarkによる解析”）、CpGメチル化の正確な測定に最適です。

Principle

PyroMark Q96 Tests はPyroMark Q96 ID Systems と組み合わせて使用することで、パイロシークエンス法による正確で定量的な変異（あるいはSNP）およびメチル化解析を実現します。これらのアッセイはPCR用プライマーとシークエンシング用プライマーで構成されています。

Procedure

パイロシークエンス法を実施する前に、ビオチン標識されたPCR産物が産生されます。ビオチン標識されたPCR 産物はストレプトアビジンでコーティングしたセファロースビーズに結合します。これらのビーズはその後PyroMark Vacuum Workstation 上のVacuum Prep Tool により捕集され、十分に洗浄された後、シークエンシング用に最適な一本鎖DNA が調製されます。PyroMark Q96 Tests はパイロシークエンス法によるメチル化解析用にデザイン済みのアッセイです。各アッセイにはビオチン標識PCR産物の作製に必要なプライマーおよびパイロシークエンス解析用のプライマーが含まれています。

Applications

PyroMark Q96 Testsはがんおよびエピジェネティクスなどの研究分野に適応可能なメチル化および変異（あるいはSNP）解析を正確かつ定量的に行ないます。

FAQs

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	Can I order the nucleotides from PyroMark Gold Reagents separately? FAQ ID -2827	View
	Which purity grade is recommended for pyrosequencing primers? FAQ ID -2832	View
	How many times can vacuum troughs be re-used with the PyroMark Vacuum Preparation Stations? FAQ ID -2848	View
	Where can I order the Streptavidin Sepharose beads for pyrosequencing? FAQ ID -2850	View
	What is the amplicon length of the PyroMark CpG LINE assay? -1	View
	What region of LINE gets targeted by the assay for PyroMark Q96 MD or PyroMark Q24? -1	View
	How many CpG sites are analyzed by the PyroMark CpG LINE assay? -1	View
	Does the PyroMark CpG LINE assay target mouse transposons as well? -1	View
	Does the PyroMark LINE assay target all LINE sequences? -1	View
	Can the PyroMark Q96 CpG LINE assay be used with an ID system? -1	View
	How many nucleotides of a homopolymer can be resolved in pyrosequencing? FAQ ID -2871	View
	Can unused wells in a pyrosequencing plate be used in the next run? FAQ ID -2872	View
	What is the reason for signals ceasing in the middle of a pyrosequencing run? FAQ ID -2875	View
	How do I reduce background peaks in the pyrosequencing pyrogram? FAQ ID -2877	View
	How do I prevent a drifting baseline in my pyrosequencing pyrogram? FAQ ID -2878	View
	What is the reason for a high substrate peak in the pyrosequencing pyrogram? FAQ ID -2879	View
	What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram? FAQ ID -2881	View

Scientific Posters

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	(EN) - Pyrosequencing and its applications	Show details

Kit Handbooks

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	(EN) - PyroMark APOE Handbook	Show details
	(EN) - PyroMark HFE Handbook	Show details
	(EN) - PyroMark MTHFR Handbook	Show details
	(EN) - PyroMark Q96 CpG LINE-1 Handbook	Show details

Brochures & Guides

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	Pyrosequencing – the synergy of sequencing and quantification	Show details

Safety Data Sheets

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	Safety Data Sheets Download Safety Data Sheets for QIAGEN product components.	View

Safety Data Sheets

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	MSDS PyroMark APOE (96)
	MSDS PyroMark HFE (96)
	MSDS PyroMark MTHFR (96)
	MSDS PyroMark Q96 CpG LINE-1 (2 x 96)
	MSDS PyroMark Q96 CpG MGMT (96)
	MSDS PyroMark Q96 CpG MLH1 (96)
	MSDS PyroMark Q96 CpG p16 (96)
	MSDS PyroMark Q96 CpG Prader-Willi (96)

Images

PyroMark p16 analysis.