製品名 Cat. no. List price:
QIAgility System HEPA/UV (incl. PC)

Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer and QIAgility software; includes installation, application training and one-year warranty on labor, travel and parts.

9001532
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QIAgility, HEPA/UV, PC, Priority

Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer and QIAgility software; includes installation, application training, Full Agreement for two years with a two business day response time and two preventive maintenance visits.

9001903
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QIAgility, HEPA/UV, PC, PrioPLUS

Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer and QIAgility software; includes installation, application training, Full Agreement for three years with a two business day response time and three preventive maintenance visits.

9001904
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QIAgility HEPA/UV (w/o PC) System

Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter) and QIAgility software; includes installation, application training and one-year warranty on labor, travel and parts. Does not include a laptop.

9001908
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QIAgility HEPA/UV (w/o PC), PrioPLUS

Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter) and QIAgility software; includes installation, application training, Full Agreement for three years with a two business day response time and three preventive maintenance visits. Does not include a laptop.

9001910
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QIAgility HEPA/UV (w/o PC), Priority

Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter) and QIAgility software; includes installation, application training, Full Agreement for two years with a two business day response time and two preventive maintenance visits. Does not include a laptop.

9001909
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QIAgility HEPA/UV Platform System PRV-1

Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer and QIAgility software; includes installation, application training and one preventive maintenance visit. One-year warranty on labor, travel and parts are also included.

9002887
Details
QIAgility HEPA/UV System PRV-1

Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter) and QIAgility software; includes installation, application training and one preventive maintenance visit. One-year warranty on labor, travel and parts also included. Does not include a laptop.

9002888
Details
QIAgility HEPA/UV (w/o PC) System

Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter) and QIAgility software; includes installation, application training and one-year warranty on labor, travel and parts. Does not include a laptop.

9001908
Inquire
QIAgility, Premium Agreement

On-site QIAgility instrument repair, including travel, labor and parts, for a period of one year. Response time of next business day. Includes one Preventive Maintenance or Inspection Service during the Premium Agreement period.

9241764
Details
QIAgility, Full Agreement

On-site QIAgility instrument repair, including travel, labor and parts, for a period of one year. Response time of two business days. Includes one  Preventive Maintenance or Inspection Service during the Full Agreement period.

9241763
Details
QIAgility, Preventive Subscription

One on-site Preventive Maintenance or Inspection Service visit for the QIAgility, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.

9241776A
Details
QIAgility, Core Agreement

For the QIAgility, one on-site repair service and one on-site Preventive Maintenance or Inspection Service, including travel, labor and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during the Core Agreement period.

9245243
Details

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機器ユーザーマニュアル
6
Important note regarding new QIAgility declaration of conformity and instrument symbols
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For use with QIAgility and QIAgility Setup Manager Software
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Alternative options for the CD drive on the QIAgility Notebook, Part Number 9026761
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For use with QIAgility Operating Software version 4.17.1
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For use with QIAgility Operating Software version 4.18

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オペレーティング・ソフトウェア
3
Instrument operating software including the QIAgility Setup Manager Gene Expression, Pathogen Detection, Sample Pooling and Generic PCR plug-in. The software offers a complete solution for qPCR setup, sample normalization, CE loading, certain liquid handling, as well as sample pooling. The Gene Expression and Pathogen Detection plug-ins are optimized for use with QIAGEN gene expression and pathogen detection kits, such as QuantiTect, QuantiFast, QuantiNova and Rotor-Gene kits. The Generic PCR plug-in allows flexible qPCR setup, liquid handling and support for the whole HID workflow.

For software installation instructions, please refer to the QIAgility Unpacking and Installation Instructions or to the QIAgility User Manual, chapter 4.4.

Important
: It is not possible to install both QIAgility Setup Manager Software and QIAgility Operating Software 4.17.1 on the same computer. Please uninstall QIAgility Operating Software 4.17.1 before installing QIAgility Setup Manager Software. For more information, contact QIAGEN Technical Services.

Note
: QIAgility Operating Software is not compatible with CAS-1200 instruments. Download the latest CAS-1200 operating software.
詳細を表示
Instrument operating software designed for flexible use in various applications. Use this software for all applications other than gene expression and pathogen detection. For gene expression and pathogen detection applications, we recommend using the QIAgility Setup Manager Software.

Important
: It is not possible to install both QIAgility Operating Software 4.17.1 and QIAgility Setup Manager Software on the same computer. Please uninstall QIAgility Setup Manager Software before installing QIAgility Operating Software 4.17.1. For more information, contact QIAGEN Technical Services.

Note
: QIAgility Operating Software is not compatible with CAS-1200 instruments. Download the latest CAS-1200 operating software.
詳細を表示
Instrument operating software designed for flexible use in various applications. Use this software for all applications other than gene expression and pathogen detection. For gene expression and pathogen detection applications, we recommend using the QIAgility Setup Manager Software.

Important: It is not possible to install both QIAgility Operating Software 4.18 and QIAgility Setup Manager Software on the same computer. Please uninstall QIAgility Setup Manager before installing QIAgility Operating Software 4.18. For more information, contact QIAGEN Technical Services.

Note: QIAgility Operating Software is not compatible with CAS-1200 instruments. Download the latest CAS-1200 operating software
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プロトコール
1
These protocol files can only be used with QIAgility Operating Software version 4.17 and are not compatible with the new QIAgility Setup Manager Software.
QIAgility Setup Manager Software offers a complete solution for qPCR setup optimized for use with QIAGEN kits. If you need a list of kits currently supported by the QIAgility Setup Manager Software, QIAGEN Technical Services will be happy to help you. Visit support.qiagen.com for country-specific contact information.


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Application Notes
1
This study tested a workflow for quantitation and qualification of AAV samples using a duplex assay on the QIAcuity dPCR instrument targeting both an insert (GFP) and the viral backbone (AAV2-ITR). With very low intra-assay and inter-assay CVs <6.5%, we demonstrate one of the main benefits of dPCR: reproducibility.
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MSDS
1
Download Safety Data Sheets for QIAGEN product components.
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Exceptional pipetting precision and accuracy spanning over a broad volume range
Exceptional pipetting precision and accuracy spanning over a broad volume range
The Artel MVS system was employed to determine the pipetting precision of the QIAgility instrument operated by the QIAgility Setup Manager Software. The QIAgility dispensed the Artel solutions (selected according to the Artel MVS User Manual) into the Artel buffer. Three runs were performed on three QIAgility instruments that were used to pipet an entire volume range (1, 2, 5, 10, 20, 50, 100, and 200 µl), in replicates of 12. For each run and volume (n=12) the coefficient of variation (CV) was calculated to determine the pipetting precision of the QIAgility. The data shows the mean of the CV values (bars; in %) ± standard deviation (S.D.) of the CV values as error bars. The red lines depict the claimed specifications.
The indicated CV values (%) demonstrate that the QIAgility instrument performs within its claimed specifications and even better.
Unrivaled pipetting precision and reproducibility of the QIAgility for master mix solutions
Unrivaled pipetting precision and reproducibility of the QIAgility for master mix solutions
A reaction mix was prepared manually using 5 parts of 2x Rotor-Gene SYBR Green PCR Master Mix and 3 parts of RNase free water (ready-made reaction mix). An experiment was then defined in the QIAgility Setup Manager for the Rotor-Gene SYBR Green PCR Kit with a total reaction volume of 25 µl and a sample input volume of 5 µl, resulting in the transfer of 20 µl of the ready-made reaction mix. The transfers were made into pre-weighed 200 µl single tubes (n=36 per instrument). The tubes were weighed again after the transfer and the weight difference was considered to calculate the transferred volume. For each instrument, the pipetting precision was determined as CV (%). The data shows the mean of the CV values (bars; in %) ± S.D. (error bars) of each individual instrument. The pipetting precision or CV, respectively, was nearly 3 % for all instruments tested.
The indicated CV values (%) demonstrate that the QIAgility instrument has high performance rate in terms of precision and repeatability when pipetting ready-made reaction mix as shown on all 4 instruments.
Comparison of manual and automated assay setup using series dilutions
Comparison of manual and automated assay setup using series dilutions
A QuantiNova SYBR Green assay setup was performed using the QIAgility operated with its Setup Manager Software and this was compared to a manual setup done in parallel. A manually prepared 1:10 dilution series from a stock of human gDNA with a concentration of 60 ng/µl was used in a ß-actin assay. Each dilution was tested in quadruplicates (n=4). The manual and QIAgility-prepared assay points were amplified in the same Rotor-Gene Q run. The data is represented as a box plot of the CT value calculated using the Q-Rex Software for each template concentration and setup employed.
A highly similar PCR performance is observed between manual and automated assay setup over a series of sample concentrations.
High system linearity of QIAgility
High system linearity of QIAgility
Human gDNA with a stock concentration of 1.5 µg/µl was diluted with the QIAgility six times in the ratio of 1:2 per dilution step using RNase free water as diluent. The dilution series was prepared in replicates of 3. The nucleic acid concentration of the dilutions was determined using the QIAxpert UV/VIS spectrophotometer (DNA QIAamp Application). The mean measured quantifications were plotted against the mean theoretical nucleic acid concentrations assuming a perfect 1:2 dilution. The resulting plot demonstrates the exceptional linearity of QIAgility dilution with a high regression coefficient of >0.999.
Quantifications of serial dilutions of gDNA prepared using the QIAgility are in high concordance with actual concentrations.