MinElute PCR Purification Kit

For purification of up to 5 μg PCR products (70 bp to 4 kb) in low elution volumes
  • Very small elution volumes
  • Fast procedure and easy handling
  • High, reproducible recoveries
  • Gel loading dye for convenient sample analysis

The MinElute PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products 70 bp – 4 kb in size. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering high yields of highly concentrated DNA. An optional pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect.

Product Cat. no. List price:
MinElute PCR Purification Kit (50)
50 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
28004
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MinElute PCR Purification Kit (250)
250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
28006
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The MinElute PCR Purification Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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MinElute操作手順|プライマーの効率的な除去|GelPilot Loading Dye|MinEluteメンブレン|pH 指示薬|Spin Column操作オプション - D|Spin Column操作オプション - E|Spin Column操作オプション - C|Spin Column操作オプション - B|Spin Column操作オプション - A|
この結合―洗浄―溶出の簡単な操作によって、簡便性が確実に高まる。|MinElute PCR Purification KitによるPCR産物の精製前(b)と精製後(a)の解析結果。1.2% TAEアガロースゲルでサンプルを解析した。M:マーカー。|GelPilot Loading Dye に入っている3種類のマーカー色素によりDNA電気泳動の至適化が容易。
|ユニークなMinEluteメンブレン構造
|溶解および結合バッファー中のpH指示薬により、DNA吸着の至適pH(pH <7.5)かどうかを簡単に確認できる。アガロースゲル電気泳動バッファーを繰り返し使用したり、間違って調製した場合に、結合溶液のpHは至適pHより高くなる。このような場合は、3M 酢酸ナトリウム、pH 5.0 を10 µl 添加してpHを簡単に調整することが可能。|QIAvac 24 Plus|QIAcube
|ルアーコネクター付きマニホールド
|QIAvac 24
|マイクロ遠心機
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パフォーマンス
The MinElute PCR Purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from DNA samples (see figure "Efficient primer removal").

The MinElute PCR Purification Kit provides spin columns for PCR product cleanup. Using a microcentrifuge or vacuum manifold, high concentration of DNA fragment (70 bp – 4 kb) is quickly achieved. (DNA fragments larger than 4 kb should be purified using the QIAquick PCR Purification Kit.)

原理

The MinElute PCR Purification Kit contains a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. 

Gel loading dye

To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure "GelPilot Loading Dye").

操作手順

The MinElute System uses a simple bind-wash-elute procedure. Binding buffer is added directly to the PCR sample or other enzymatic reaction, and the mixture is applied to the MinElute spin column. The binding buffer contains a pH indicator, allowing easy determination of the optimal pH for DNA binding (see figure "pH Indicator Dye"). Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Handling

MinElute spin columns are designed to provide two convenient handling options (see flowchart "MinElute procedure"). The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as QIAvac 24 Plus or QIAvac 6S with QIAvac Luer Adapters and can also be fully automated on the QIAcube (see figures "Spin column handling options A, B, C, D, and E").

アプリケーション

DNA fragments purified with the MinElute System are ready for direct use in all applications, including:

  • Sequencing, including next generation sequencing
  • Microarray analysis
  • Ligation and transformation
  • Restriction digestion
  • Labeling

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Safety Data Sheets
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Images
MinElute Procedure
MinElute procedure.

This simple bind–wash–elute procedure ensures greater convenience.

Efficient primer removal using the MinElute PCR Purification Kit
Efficient primer removal.
Analysis of PCR products before (b) and after (a) purification with the MinElute PCR Purification Kit. Samples were analyzed on a 1.2% TAE agarose gel. M: markers.
GelPilot Loading Buffer
GelPilot Loading Dye.
GelPilot Loading Dye contains three tracking dyes to facilitate optimization of DNA resolution.
MinElute Membrane Assembly
MinElute membrane.
Unique MinElute membrane assembly.
ILLU_0104_MinElute
pH Indicator Dye.
pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case, the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.

Spin column handling options — D.
QIAvac 24 plus.
Spin column handling options — E.
QIAcube.
QIAprep Spin Column handling options
Spin column handling options — C.
Manifold with luer connectors.
QIAprep Spin Column handling options
Spin column handling options — B.
QIAvac 24.
QIAprep Spin Column handling options
Spin column handling options — A.
Microcentrifuge.