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QIAsymphony DSP AXpH DNA Kit

For automated isolation and purification of DNA from liquid-based cytology media

  • Novel, prefilled reagent cartridges for ease of use
  • High reproducibility through standardized processing
  • Continuous loading of up to 96 samples per run
  • Bar codes enable tracking of samples and reagents

The QIAsymphony DSP AXpH DNA Kit, in combination with the QIAsymphony SP, enables automated DNA purification from liquid-based cytology media for in vitro diagnostic use. Optimized protocols provide DNA eluates that are ready to use in downstream applications. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents. The QIAsymphony SP performs all steps of the sample preparation procedure. Up to 96 samples, in batches of up to 24, are processed in a single run.

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Cat No./ID: 937156
QIAsymphony DSP AXpH DNA Kit (192)
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For 192 DNA preps: includes 2 reagent cartridges and enzyme racks and accessories
This product will be discontinued as of June 30, 2021 or until stocks last.
 

The QIAsymphony DSP AXpH DNA Kit is intended for in-vitro diagnostic use.



Product Details

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Schematic of the QIAsymphony SP principle.
The QIAsymphony SP processes a sample containing magnetic particles as follows: A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod cover is positioned above another well and the magnetic particles are released. The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process up to 24 samples simultaneously. Steps 1 and 2 are repeated several times during sample processing.
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CE-IVD-marked.
 
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In vitro diagnostic medical device.
 
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QIAsymphony DSP AXpH procedure.
 
Principle
QIAsymphony DSP AXpH technology combines the speed and efficiency of pH-driven anion exchange chromatography with the convenient handling of magnetic particles (see "Schematic of the QIAsymphony SP principle"). Innovative, ready-to-run reagent cartridges are prefilled with all reagents required for the purification procedure and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination.
Procedure

The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute (see "QIAsymphony DSP AXpH procedure"). Worktable setup is rapid, which saves valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, allowing complete cost control.

Protocols for use with the QIAsymphony DSP AXpH DNA Kit
ProtocolSample type Volume processed
AXpH2000_DSP PreservCyt samples 2 ml
SP2000_DSP Diluted SurePath postgradient samples 2 ml

Applications

The QIAsymphony DSP AXpH DNA Kit in combination with the QIAsymphony SP enables purification of DNA from liquid-based cytology samples, such as cervical swabs collected in SurePath (BD) or PreservCyt (Cytyc/Hologic) media.

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Application/Protocol Documents (5)
(EN) - AXpH2000_V5_DSP
For use with software version 4.0 and higher
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(EN) - SP2000_V2_DSP
For use with software version 4.0 or higher
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QIAsymphony SP Protocol Sheet AXpH2000_V5_DSP protocol
For use with software version 4.0 or higher 
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QIAsymphony SP Protocol Sheet SP2000_V2_DSP protocol
For use with software version 4.0 or higher 
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QIAsymphony SP Protocol Sheet PC_AXpH_HC2_V1_DSP protocol
For use with software version 4.0 or higher 
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