QuantiTect Virus Kits provide highly sensitive real-time PCR analysis of viral nucleic acids (RNA and/or DNA) and internal controls using sequence-specific probes. Reactions can be carried out with or without a reverse-transcription step, enabling flexible design of multiplex assays to detect RNA targets, DNA targets, or both RNA and DNA targets. Follow the protocol in the handbook for fast and reliable results.
Kits are available with or without ROX passive reference dye in the master mix (see table).
Choosing the right QuantiTect Virus Kit
|ROX dye||Kit ||Compatible cyclers|
|Supplied in master mix ||QuantiTect Virus Kit ||All cyclers from Applied Biosystems except Applied Biosystems 7500|
|Supplied in separate tube ||QuantiTect Virus +ROX Vial Kit ||Applied Biosystems 7500 and cyclers from|
Bio-Rad, Cepheid, Eppendorf, QIAGEN, Roche, Agilent, and other suppliers
For fast and highly sensitive end-point one-step RT-PCR applications, including virus detection, we recommend using the QIAGEN OneStep RT-PCR Kit.