The QIAquant instruments enable quantitative real-time PCR in either intercalating dye or hydrolysis probe-based assays and in tubes, or in a 96- and 384-well plate format. QIAquant instruments are designed for convenience, ease of use and reliability, from run setup to interpretation of results. A QIAquant instrument will readily integrate in any new or existing real-time PCR workflow.
Flexibility of chemistries and plasticware
The QIAquant instruments work as an open system and can be used in combination with any compatible real-time PCR kits. SBS sample blocks make the QIAquant systems compatible with the majority of tubes, strips and low- or normal- profile plates, skirted, half-skirted or unskirted. For best performance, QIAGEN recommends the used of dedicated qPCR plates with white well plates and qPCR compatible foils or seals.
Visit the QuantiNova products overview page to discover QIAGEN's leading real-time PCR solutions.
Visit the QIAquant consumables page for qPCR plates and foils.
Multiplex PCR for co-detection of up to 5 different targets
The QIAquant instruments come with up to 5 color channels for multiplexing in real-time PCR applications. The QIAquant Software comes with built-in optimized generic cross-talk compensation. Users can create customized color compensation files for specific set of dyes if necessary.
|Channel ||Excitation (nm) ||Detection (nm) ||Examples of fluorophores detected |
|Blue ||465±15 ||524±12 ||FAM™, SYBR® Green, EvaGreen® |
|Green ||510±15 ||565±15 ||JOE™, HEX™, VIC® |
|Orange (5plex versions only) ||560±15 ||610±15 ||ROX™, Texas Red® |
|Red (5plex versions only) ||625±10 ||680±15 ||Cy5®, Alexa Fluor® 647 |
|NIR 1 (5plex versions only) ||625±10 ||710±20 ||Cy5.5®, Quasar® 705 |
Simple operation with the QIAquant software
The QIAquant instruments are operated via the QIAquant software, using either the desktop version or the touchscreen version. The QIAquant software is suitable for use by novice researchers, while maintaining the highly complex data analysis functions required by advanced researchers. The comprehensive QIAquant software package supports all current state-of-the art real-time analysis procedures from basic to advanced algorithms. This provides complete freedom to analyze your valuable experimental data and increases the reliability of your results. Data security is assured and all process steps are trackable from starting the run to exporting the results. QIAquant Software is compatible with Audit trail for 21CFR part 11 compliance.
Maintenance and hassle free
The optical system of the QIAquant instrument involves individual illumination and detection of a sample with a PMT and use of a reference dye such as ROX is not required. The optical system does not need to be calibrated on a regular basis. The QIAquant instruments are factory-calibrated for optical and thermal accuracy. (QIAGEN does recommend yearly preventive maintenance.)