RNase A
用于在DNA制备时进行RNase消化
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RNase A
目录编号 / ID. 19101
2.5 mL (100 mg/mL; 7000 units/mL, solution)
RNase A 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。
✓ 全天候自动处理在线订单
✓ 博学专业的产品和技术支持
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特点
- Selectively cleaves single-stranded RNA
- Active under a wide range of reaction conditions
- Endonuclease-free and quality-controlled
- Does not need to be boiled before use
产品详情
QIAGEN Ribonuclease A（RNase A）不含核酸内切酶，并经过质量控制，适用于在质粒纯化实验中消化RNA。这一即用型溶液与QIAGEN质粒DNA纯化试剂盒中的RNase具有相同规格参数。使用时需要将溶液等分、在Buffer P1中稀释至合适浓度。
绩效
QIAGEN Ribonuclease A is Endonuclease-free and quality-controlled for use in plasmid purification procedures for the digestion of RNA.
RNase A is widely used to remove contaminating RNA during the isolation of plasmid and genomic DNA. RNase A is also used to remove contaminating RNA from DNA samples after extraction or PCR, especially if downstream applications (e.g., cloning or sequencing) require RNA-free DNA.
RNase A is very active under a wide range of reaction conditions and is difficult to inactivate.
QIAGEN RNase A does not need to be boiled before use.
| Feature | RNase A |
|---|---|
| Quantity | 2.5 mL |
| Concentration | 100 mg/; 7000 units/ml, solution |
| Unit definition | One Kunitz unit is the amount of enzyme causing the hydrolysis of RNA at a rate such that the velocity constant (k) equals unity at 25°C and pH 5.0 |
程序
Before use in plasmid and DNA purification, centrifuge QIAGEN RNase A briefly, and then add into Buffer P1 to obtain a final concentration of 100 μg/mL. Store Buffer P1 at 2–8°C after addition of RNase A. For detailed instructions on RNase A usage during plasmid and DNA purification, please refer to the corresponding kit handbook.
应用
- Plasmid and genomic DNA preparation
- Removal of RNA contamination from recombinant protein preparations
- Cleanup of RNA from enzymatic reactions
资源
Brochures & Guides (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
常见问答
3314-What is the concentration of RNase A sold separately?