Plant samples are mechanically disrupted using a homogenizer such as the TissueLyser. The resulting powder is resuspended in lysis buffer, thoroughly mixed, and centrifuged. Lysates are transferred to a 96-well microplate containing MagAttract magnetic particles, and genomic DNA selectively binds to the surface of the particles (see " MagAttract 96 DNA Plant Core procedure
"). After washing, pure genomic DNA is eluted with low-salt buffer, and is ready for use in downstream applications. The MagAttract 96 DNA Plant Kit is designed for use with the BioRobot Plant Science system for fully automated DNA purification. QIAGEN also offers an automated protocol using the BioRobot RapidPlate workstation, and a manual protocol for evaluation purposes.