Get 5% off the enzyme of your choice.
Use the code ENZ2526 at checkout. Offer valid from December 1, 2025, to March 31, 2026. T&Cs* apply.
RNase A
カタログ番号 / ID. 19101
2.5 ml (100 mg/ml; 7000 units/ml, solution)
*Offer valid on orders placed from December 1, 2025, to March 31, 2026, or while stocks last. Offer void where prohibited and cannot be combined with any other promotion. Offer is only valid in the following countries: Australia, Austria, Belgium, Canada, Denmark, Finland, France, Germany, Italy, Luxembourg, Netherlands, New Zealand, Norway, Poland, South Africa, Sweden, Switzerland, United Kingdom, United States of America. Freight and delivery costs are not included. It is the customer's responsibility to ensure that acceptance of this offer will not violate any internal policies of the customer's organization and applicable laws and regulations.
RNase Aは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。
Get 5% off the enzyme of your choice.
Use the code ENZ2526 at checkout. Offer valid from December 1, 2025, to March 31, 2026. T&Cs* apply.
特徴
- Selectively cleaves single-stranded RNA
- Active under a wide range of reaction conditions
- DNase-free and quality-controlled
- Does not need to be boiled before use
製品詳細
QIAGEN Ribonuclease A（RNase A）は厳しい品質管理を受けたエンドヌクレアーゼフリー酵素で、プラスミド精製操作中のRNA分解に最適です。この即使用可能の溶液は、全てのQIAGENのプラスミドDNA精製キットに含まれるRNAaseと同じ仕様です。この溶液はBuffer P1で希釈することで適正な濃度に調製します。
パフォーマンス
QIAGEN Ribonuclease A is DNase-free and quality-controlled for use in plasmid purification procedures for the digestion of RNA.
RNase A is widely used to remove contaminating RNA during the isolation of plasmid and genomic DNA. RNase A is also used to remove contaminating RNA from DNA samples after extraction or PCR, especially if downstream applications (e.g., cloning or sequencing) require RNA-free DNA.
RNase A is very active under a wide range of reaction conditions and is difficult to inactivate.
QIAGEN RNase A does not need to be boiled before use.
| Feature | RNase A |
|---|---|
| Quantity | 2.5 mL |
| Concentration | 100 mg/; 7000 units/ml, solution |
| Unit definition | One Kunitz unit is the amount of enzyme causing the hydrolysis of RNA at a rate such that the velocity constant (k) equals unity at 25°C and pH 5.0 |
操作手順
Before use in plasmid and DNA purification, centrifuge QIAGEN RNase A briefly, and then add into Buffer P1 to obtain a final concentration of 100 μg/mL. Store Buffer P1 at 2–8°C after addition of RNase A. For detailed instructions on RNase A usage during plasmid and DNA purification, please refer to the corresponding kit handbook.
アプリケーション
- Plasmid and genomic DNA preparation
- Removal of RNA contamination from recombinant protein preparations
- Cleanup of RNA from enzymatic reactions
リソース
安全データシート (1)
パンフレットとガイド (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
よくある質問
3314-What is the concentration of RNase A sold separately?