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特徴
- Selectively cleaves single-stranded RNA
- Active under a wide range of reaction conditions
- Endonuclease-free and quality-controlled
- Does not need to be boiled before use
製品詳細
QIAGEN Ribonuclease A（RNase A）は厳しい品質管理を受けたエンドヌクレアーゼフリー酵素で、プラスミド精製操作中のRNA分解に最適です。この即使用可能の溶液は、全てのQIAGENのプラスミドDNA精製キットに含まれるRNAaseと同じ仕様です。この溶液はBuffer P1で希釈することで適正な濃度に調製します。
パフォーマンス
QIAGEN Ribonuclease A is Endonuclease-free and quality-controlled for use in plasmid purification procedures for the digestion of RNA.
RNase A is widely used to remove contaminating RNA during the isolation of plasmid and genomic DNA. RNase A is also used to remove contaminating RNA from DNA samples after extraction or PCR, especially if downstream applications (e.g., cloning or sequencing) require RNA-free DNA.
RNase A is very active under a wide range of reaction conditions and is difficult to inactivate.
QIAGEN RNase A does not need to be boiled before use.
| Feature | RNase A |
|---|---|
| Quantity | 2.5 mL |
| Concentration | 100 mg/; 7000 units/ml, solution |
| Unit definition | One Kunitz unit is the amount of enzyme causing the hydrolysis of RNA at a rate such that the velocity constant (k) equals unity at 25°C and pH 5.0 |
操作手順
Before use in plasmid and DNA purification, centrifuge QIAGEN RNase A briefly, and then add into Buffer P1 to obtain a final concentration of 100 μg/mL. Store Buffer P1 at 2–8°C after addition of RNase A. For detailed instructions on RNase A usage during plasmid and DNA purification, please refer to the corresponding kit handbook.
アプリケーション
- Plasmid and genomic DNA preparation
- Removal of RNA contamination from recombinant protein preparations
- Cleanup of RNA from enzymatic reactions
リソース
Safety Data Sheets (1)
Certificates of Analysis (1)
よくある質問
3314-What is the concentration of RNase A sold separately?