QIAamp MinElute ccfDNA Kits

ヒト血漿あるいは血清から遊離DNAを濃縮・精製

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QIAamp MinElute ccfDNA Midi Kit (50)

Cat. No. / ID: 55284

For 50 preps (4 or 5 ml sample input volume each): QIAamp UCP MinElute Columns, QIAGEN Proteinase K, Magnetic Bead Suspension, Buffers, Bead Elution Tubes, and Collection Tubes (1.5 ml and 2 ml)

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￥155,000

KitAccessories

QIAamp MinElute ccfDNA Kit

Accessories

Volume

5–10 ml

1–4 ml

Quantity

日本では本採血管は医療機器となります。販売に関しては日本ベクトン・ディッキンソン株式会社へお問い合わせください。

QIAamp MinElute ccfDNA Kitsは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

断片化DNA を効率的に回収
サンプルインプット量を増やし（最大10 ml）、溶出量を減らす（最少20 μl）ことで核酸の高濃縮を実現
高収量で確実に低存在量のバイオマーカーを検出
有機溶媒抽出およびアルコール沈殿不要、夾雑物および阻害物質を効率よく除去

Product Details

QIAamp MinElute ccfDNA Kits は、血清／血漿中の遊離DNA（ccfDNA; circulating cell-free DNA）を簡単に濃縮・精製できます。精製ステップは QIAcube による自動化が可能です。

Performance

血漿、血清および体液中のccfDNA の存在量は大変に低く、通常1,000 bp 未満の短い断片として存在しています（ヒト血漿中 ccfDNA 1 ～ 100 ng/ml）。そのため単離は非常に困難です。QIAamp MinElute ccfDNA Kits は、少量であっても、血清および血漿サンプルから高品質のccfDNAを効率的に精製し、優れた結果を提供します。他のキットと比較して、QIAamp MinElute ccfDNA Kits は、最大のccfDNA 収量を示しました（図　）。遊離DNA（ccfDNA）を磁性ビース法により、幅広い容量のサンプル（1 ～10 ml）から高度な濃縮が可能です（図　）。また、精製ステップは定評のあるカラムメンブレン（QIAamp UCP MinElute Columns）で、溶出量も20〜80 µl まで様々に対応できます。QIAamp MinElute ccfDNA Mini Kitは1〜4 ml、QIAamp MinElute ccfDNA Midi Kitは4〜10 ml のサンプル量に至適化されています。QIAamp UCP MinElute Column は20塩基程度の短い断片化DNAを結合する事が出来ますが、収量についてはサンプル中のccfDNA の濃度とサンプルの状態に依存します。

See figures

Highest yield of ccfDNA

Flexible input volume

Principle

血液中の血中循環腫瘍DNA（ctDNA）や、母体血液中の胎児DNAのようなccfDNA は、通常1,000 bp 未満の短い断片として血清または血漿中に存在しています（ヒト血漿中 ccfDNA 1 ～ 100 ng/ml）。特にctDNA は腫瘍ゲノム全体の情報をもち、有効な臨床応用の可能性があります。血漿、血清などの体液中のccfDNA 濃度は、大変低く、その濃縮、精製には依然として大きな課題があります。QIAamp MinElute ccfDNA Kits は、QIAamp Circulating Nucleic Acid Kit の性能を継承しながら、磁性ビーズ方式で簡便にccfDNA 濃縮を行ない、大量サンプル量（1～10 ml）への適応を可能にしました。ccfDNA 精製のステップは QIAcubeで自動化することができます。

Procedure

本キットは迅速かつ簡便な磁性ビーズ方式によるccfDNA 濃縮ステップにより、サンプル間のクロスコンタミネーションを排除し、潜在的に感染性のあるサンプルを取り扱う際の安全性を高めます。簡便な操作で、複数サンプルの同時処理に適しており、24 サンプルを1 時間未満で精製できます。QIAamp MinElute ccfDNA Mini Kit は2 ml のサンプル処理に至適化されたプロトコールを採用しており、4 ml のサンプルを処理にする場合には1 キットあたりのサンプル処理数は25 回となります。同様にQIAamp MinElute ccfDNA Midi Kit は4 ml のサンプル処理に至適化され、10 ml のサンプルを処理する場合には、1 キットあたりのサンプル処理数は25 回分となります。
Ultra-Clean Water で溶出された溶出液は、タンパク質などの不純物を含まない核酸抽出液で、–15 ～ –30℃での即時保存も可能です。
本キットは、血清および血漿サンプルから最大のccfDNA 収量、高濃度、最短時間で、高品質ccfDNA を得られていることを示しています（図、、）。

精製ステップはQIAcubeによる自動化が可能です。

See figures

Faster access to ccfDNA

Highest yield of ccfDNA

Highest ccfDNA concentration

Applications

QIAamp MinElute ccfDNA Kits は、ヒト血清／血漿中ccfDNA の効率的な精製に至適な製品です。

Supporting data and figures

Faster access to ccfDNA

Comparison of sample processing time (for 4 samples of 4 ml plasma each, using commercially available kits) shows that the QIAamp MinElute ccfDNA Kit provided the fastest processing.

Resources

Download Safety Data Sheets for QIAGEN product components.

FAQ

The QIAsymphony PAXgene Blood ccfDNA Kit can be sued with 2 protocol lines. The standard protocols (STA) predominantely isolate small ccfDNA fragments. The large fragment protocols (LAF) efficiently co-purify large and small ccfDNA fragments. Both lines include a protocol for 2.4 ml and another for 4.8 ml plasma input.

FAQ ID - 3654

The elution volume of the standard protocols (STA) is fixed at 60 µl. The large fragment protocols (LAF) enable elution in 3 different volumes: 60, 100 and 150 µl.

FAQ ID - 3655

The expiration date is printed on the kit label and encoded in the bar code of the reagent cartridges.

FAQ ID - 3651

For overview and further information on the Globally Harmonized System of Classification and Labelling of Chemicals (GHS) please visit www.qiagen.com/ghs .

FAQ ID - 3428

Whole blood drawn into PAXgene Blood ccfDNA Tubes can be stored for at least 24 hours at 35°C. Higher temperatures will lead to lysis of blood cells and release of genomic DNA into plasma.

FAQ ID - 3640

To date, ccfDNA from blood samples collected and processed using the PAXgene Blood ccfDNA System have been successfully tested in PCR, including multiplex qPCR, and NGS applications.

FAQ ID - 3658

The recommended sample input format is the 14 ml Falcon® polystyrene round bottom tube 17 x 100 mm supplied by Corning (formerly supplied by BD).

FAQ ID - 3653

No. Freezing blood drawn into PAXgene Blood ccfDNA Tubes results in lysis of red and white blood cells and release of genomic DNA.

FAQ ID - 3642

Yes, the inside of PAXgene Blood ccfDNA Tubes is sterilized during production.

FAQ ID - 3636

The expiration date is printed on the tube label and on the shelf carton.

FAQ ID - 3633

When closed properly with the provided strips and stored at room temperature (15–25°C), a reagent cartridge can be used up to 6 weeks after the initial run.

FAQ ID - 3650

Yes. Whole blood in PAXgene Blood ccfDNA Tubes can be stored at 8–15°C for up to 7 days. Storing blood in PAXgene Blood ccfDNA Tubes below room temperature does not extend stability.

FAQ ID - 3641

If cryoprecipitates cannot be resolved completely by vortexing, the sample should be centrifuged to prevent precipitates from blocking the column. To ensure that no cfDNA is co-precipitated, centrifugation can be performed at a lower speed (at 6,800xg for 3 minutes at room temperature, as stated in the Protocol Sheet for the QS DSP Circulating DNA Kit).

FAQ ID - 3646

Plasma generated from whole blood collected into PAXgene Blood ccfDNA Tubes can be stored at 2–8°C for up to 14 days. We recommend freezing plasma at −20°C or −80°C for longer storage periods (long-term storage studies are ongoing).

FAQ ID - 3644

The yield of ccfDNA depends on the target ccfDNA. Nevertheless, yields obtained from PAXgene Blood ccfDNA Tubes are comparable to those obtained from the same volume of plasma separated directly after drawing blood into a spray-dried EDTA tube. In the PAXgene Blood ccfDNA Tube, however, this yield remains stable for up to 7 days when the tube is stored at 15–25°C or for up to 24 hours when stored at 35°C. In contrast, genomic DNA is released from lysed and apoptotic cells in the unstabilized contents of the EDTA tube soon after blood draw.

FAQ ID - 3637

Yes. Further information on phlebotomy technique with the PAXgene Blood ccfDNA Tube can be found in the PAXgene Blood ccfDNA Tube Product Circular under the Resources tab.

FAQ ID - 3626

The number of extractions that can be performed with a kit is independent of sample volume. A kit contains sufficient reagents for 192 extractions.

FAQ ID - 3649

If the instructions for use are followed, there is no possibility of backflow during phlebotomy and thus, no possibility of reagent going into the patient’s arm.

FAQ ID - 3629

A complete 10 ml blood draw ensures optimal results. However, depending on the downstream application and target ccfDNA fragment size, tubes filled with as little as 6.5 ml whole blood can be used without adversely affecting results.

FAQ ID - 3638

Yes, as long as the phlebotomy instructions included with the PAXgene Blood ccfDNA Tubes are properly followed.

FAQ ID - 3630

The PAXgene Blood ccfDNA Tube contains a proprietary formulation that prevents blood coagulation and the release of genomic DNA from blood cells into plasma. Contents of the tube may cause irritation to the eyes, respiratory system and skin. Please refer to the PAXgene Blood ccfDNA Tube Product Circular. For additional information, visit www.preanalytix.com or review the Safety Data Sheet under the Resources tab.

FAQ ID - 3632

The blood draw volume is 10 ml and the stabilization reagent volume is 1.5 ml. Thus, a filled tube holds a volume of 11.5 ml.

FAQ ID - 3631

The QIAsymphony PAXgene Blood ccfDNA Kit is designed to be used with plasma generated from whole blood collected into PAXgene Blood ccfDNA Tubes.

FAQ ID - 3652

Thaw the tubes at room temperature (15–25°C). To minimize the formation of cryoprecipitates, do not thaw at lower temperatures (e.g., 4°C). Alternatively, thaw plasma for 30 minutes at 30°C to completely avoid the formation of cryoprecipitates.

FAQ ID - 3645

Yes. Following a standard phlebotomy protocol, blood is collected into the evacuated tube using a blood collection set and a standardized needle holder that can accommodate a 16 mm diameter tube. Make sure that blood has stopped flowing into the tube before removing the tube from the holder.

For more information on phlebotomy technique, please refer to the PAXgene Blood ccfDNA Tube Product Circular under the Resources tab.

FAQ ID - 3635

No. However, a second centrifugation step helps remove traces of buffy coat that may be accidentally transferred when aspirating plasma after the first centrifugation step.

FAQ ID - 3643

Eluates should be stored at −20°C (long-term storage studies are ongoing).

FAQ ID - 3657

Yes, a customized protocol for elution with 50 µl is available on request.

FAQ ID - 3656

Since the Qproteome Plasma Membrane Protein procedure requires 1.5 ml/2.0 ml reaction tubes, we recommend using the follwing magnets for handling the magnetic beads:

QIAGEN’s Single-Tube Magnet
QIAGEN’s 12-Tube Magnet

Alternatively, comparable tube magnets or a standard clip-board magnet should also work.

FAQ ID -1193

Whole blood drawn into PAXgene Blood ccfDNA Tubes can be stored at 15–25°C for up to 7 days.

FAQ ID - 3639

The Globally Harmonized System (GHS) is an initiative by the United Nations aiming to harmonize chemical hazard communication worldwide. Hazards symbols accompanying products are changing to those of the GHS. Information on the adoption of this new system is available on our GHS Hazard Labeling pages.

FAQ ID - 3430

The normal protocols enable processing 2.4 or 4.8 ml plasma. Other volumes can be accommodated using customized protocols.

FAQ ID - 3648

Store PAXgene Blood ccfDNA Tubes at room temperature (15–25°C) and protect them from direct sunlight.

FAQ ID - 3634

When used in accordance with instructions, the blood collection set eliminates the possibility of patient contact with the reagent in the tube due to backflow of blood.

FAQ ID - 3628