PAXgene Blood miRNA Kit

全血からのmiRNAおよびトータルRNAの精製

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PAXgene Blood miRNA Kit (50)

Cat. No. / ID: 763134

For 50 RNA preps: PAXgene Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, RNase-Free DNase, RNase-Free Reagents and Buffers; to be used in conjunction with PAXgene Blood RNA Tubes

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￥87,500

研究用途専用。診断用ではありません。いかなる主張または表示も、疾病の診断、予防、治療のための情報を提供することを目的としてはいません。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

全血の採取、安定化、精製までのトータルシステム
miRNA とトータルRNA の効率的な精製
18 ～ 25℃ で最高3 日間まで安定なRNA
-20℃あるいは-70℃で少なくとも50ヶ月安定

Product Details

PAXgene Blood miRNA Systemは、約18 ヌクレオチド以上のRNA を含むトータルRNA 精製用にPAXgene Blood miRNA Kitおよび血液採取、安定化、輸送用にパクスジーンRNA 採血管（日本ベクトン・ディッキンソン株式会社、cat. no. 762165）で構成されています。精製は、シリカベースのRNA精製テクノロジーを用いてスピンカラムフォーマットで行なわれます。本精製法はマイクロ遠心機を用いたマニュアル操作、あるいはQIAcubeを用いた自動化も可能です。本キットは、専用採血管と併用することで、血液の採取、安定化からmiRNA を含む高品質なトータルRNA 精製までを統合するプレアナリティカルソリューションを提供します。

Performance

パクスジーンRNA 採血管に採取した血液は、RNA 分解や転写量の有意な変化なしに、15 ～ 25℃で最高72 時間、2～8℃で最高5 日間、-20℃または-70℃で最短50 ヶ月安全に保存および輸送可能です。安定化した血液から、18 nt 以上のトータルRNA（miRNA を含む）をマニュアルあるいはQIAcubeによる自動化で精製することができます（図“”）。様々なmiRNAが、QIAGEN miScript PCR System（図“ ”）を用いて、あるいはその他のリアルタイムRT-PCRシステムで解析できます。

See figures

High yields of small RNA species.

Analysis of miRNA species.

Principle

血液サンプルはパクスジーンRNA 採血管で採血します。本チューブに入っている試薬により、赤血球は溶解、細胞RNAは即座に安定化され、遺伝子発現プロファイルが保存されます。RNAの安定化は、ダウンストリームの遺伝子発現解析の信頼性において重要です。安定化なしでは、採血後すぐにRNA 分解や転写物のアップレギュレーションやダウンレギュレーションが起こります。

Procedure

PAXgene Blood miRNA Kitを用いて、実績のあるPAXgene シリカメンブレンテクノロジーにより、安定化した血液から18 nt 以上のトータルRNA（miRNA を含む）を精製することができます。精製はマニュアルフォーマットで行なうことも、QIAcubeによる自動化も可能です（図“QIAcube”）。

See figures

Applications

精製RNA は即使用可能で、以下のようなダウンストリームアプリケーションに最適です：

RT-PCR およびリアルタイムRT-PCR
ディファレンシャルディスプレイ
cDNA合成
ノーザン、ドット、スロットブロット解析
プライマーエクステンション
Poly A⁺ RNA selection
RNase およびS1 nuclease protection
マイクロアレイ解析

Supporting data and figures

Analysis of miRNA species.

Blood was collected in PAXgene Blood RNA Tubes and stored for 20–24 hours at room temperature before freezing at –15 to–30°C. RNA was purified using the PAXgene Blood RNA Kit or the PAXgene Blood miRNA Kit, as indicated. miRNA species were analyzed using the QIAGEN miScript PCR System, with the indicated miScript Primer Assays. The lower C_T values for RNA purified using the PAXgene Blood miRNA Kit indicate higher amounts of those miRNA species.

Analysis of miRNA using miRCURY LNA miRNA Probe PCR Assays with miRCURY LNA miRNA Probe PCR System

Specifications

Features	Specifications
Applications	RT-PCR and real-time RT-PCR, differential display, cDNA synthesis, northern, dot, and slot blot analyses, primer extension, microarray analysis
Elution volume	80 µl
Time per run or per prep	Manual: 90 min/12 samples; Automated: 125 min/12 samples, Reduced hands-on time.
Technology	Silica technology
Main sample type	Whole blood
Yield	3–18 µg/2.5 ml sample
Format	Spin column
Processing	Manual (centrifugation) or automated on QIAcube instruments
Sample amount	2.5 ml

Resources

For collection, transport, and storage of whole blood and stabilization and purification of intracellular RNA

For purification of intracellular RNA, including miRNA, from whole blood

Download Safety Data Sheets for QIAGEN product components.

Voss et al., AMP 2009

Voss et al., qPCR 2009

Kruhoffer et al., AMP 2007

This note is to inform you that the street address for PreAnalytiX GmbH has changed from “Feldbachstrasse” to “Garstligweg 8”. Please be informed that the update of the product labeling to the new address is ongoing.

In order to reduce paper consumption and oblige the growing number of customers requesting an environmentally friendly alternative to traditionally printed handbooks, we are now providing kit handbooks for Research Use Only (RUO) PreAnalytiX kits on our website only.

Comparison of miRNA recovery from the PAXgene Blood miRNA System and the Tempus Blood RNA Tube processed with the Tempus Spin RNA Isolation Kit

Typical total RNA yields from PAXgene Blood RNA Tubes processed with the PAXgene Blood miRNA Kit

For purification of miRNA and total RNA from whole blood.

For purification of intracellular RNA, including miRNA, from whole blood

For collection, transport, and storage of whole blood and stabilization and purification of intracellular RNA

Voss et al., qPCR 2009

Kruhoffer et al., AMP 2007

Voss et al., AMP 2009

For purification of miRNA and total RNA from whole blood.

FAQ

You can find additional information relating to the PreAnalytiX PAXgene products on the PreAnalytiX website .

FAQ ID - 3515

No. The PAXgene Blood miRNA Kit is ‘For Research Use Only’. Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

FAQ ID -2494

Typical yields of RNA isolated from 2.5 ml healthy, human, whole blood (4.8 x 10⁶ –1.1 x 10⁷ leukocytes/ml) are ≥3 μg for >95% of the samples processed (Refer to Technical Note) . Since yields are highly donor-dependent, individual yields may vary.

FAQ ID - 3481

No. The PAXgene Blood RNA System has been optimized for cellular RNA only. PreAnalytiX offers a dedicated system for collection and isolation of genomic DNA from human whole blood (see PAXgene Blood DNA System) .

FAQ ID - 3483

For the isolation of microRNA (miRNA) specifically, we have developed the miRNeasy Mini Kit and the miRNeasy 96 Kit for isolation from cells and tissues. We also have the PAXgene Blood miRNA and Tissue miRNA kits for isolation from blood stored in PAXgene Blood RNA tubes and PAXgene Tissue Containers, respectively. Other miRNA isolation supplementary protocols can also be found by searching our comprehensive protocols at http://www.qiagen.com/literature/default.aspx?WT.svl=m.

FAQ ID -115

Up to 12 samples can be processed in a single run.

FAQ ID - 3482

If the PAXgene Blood miRNA Kit is used, an enrichment of small RNAs as part of the total RNA is achieved. The enrichment may vary between different miRNA species and samples, but on average an enrichment of about three or more CT-values can be expected.

FAQ ID -2495

Typically, the amount of genomic DNA (gDNA) in eluates of the PAXgene Blood miRNA Kit is very low as long as the kit is stored and used according to the instructions of the PAXgene Blood miRNA Kit Handbook. For downstream applications that are especially sensitive to gDNA contamination, a specialized protocol for the QIAcube helps reduce gDNA content further and can be downloaded from the QIAcube product page.

FAQ ID - 3637

2.5 ml whole human blood collected in PAXgene Blood RNA Tubes

FAQ ID - 3478

Stability data are not yet available, but a stability study was started.

FAQ ID - 3480

The kit can be used for 50 RNA isolations.

FAQ ID - 3477

Yes. Samples have to be incubated at least 2 h at room temperature (18-22°C). Incubation of the PAXgene Blood RNA Tube overnight may increase yields slightly in some cases. Room temperature storage for up to three days has no effect on the RNA profile. During the preparation of the RNA the manual pretreatment consist of a centrifugation and a pellet resuspenion step (see the PAXgene Blood miRNA Handbook ).

FAQ ID - 3479