PAXgene Blood DNA Tubes

Thaw the PAXgene Blood DNA Tubes in a wire rack at ambient temperature (18–25ºC) for approximately two hours or at up to 37°C in a water bath for approximately 15 minutes. Carefully invert the thawed PAXgene Blood DNA Tubes 10 times.

Blood samples in PAXgene Blood DNA Tubes can be stored at room temperature (18–25°C) for up to 14 days or at 2–8°C for up to 28 days. For long-term storage, samples should be frozen at –20°C.

The typical A260/A280 ratio is from 1.7 to 1.9. The DNA molecules are up to 200 kb in size, with fragments of 50–150 kb predominating.

No, a blood collection set is not required to use PAXgene Blood DNA Tubes. Further information on phlebotomy technique with PAXgene Blood DNA Tubes IVD can be found in the product handbook.

Yes, the PAXgene Blood DNA Tubes can be stored at 4°C for up to 28 days after blood collection.

Yes, samples in PAXgene Blood DNA Tubes can be stored at 30°C for up to 3 days after blood collection. However, it is not recommended to store blood samples at temperatures higher than 30°C and storage at high temperatures should not exceed 3 days.

The shelf life of unused PAXgene Blood DNA Tubes is 1 year. The expiration date is printed on the tube label and on the shelf carton. Store PAXgene Blood DNA Tubes at room temperature (18–25°C).

The typical A₂₆₀/A₂₈₀ ratio is from 1.7 to 1.9.

Yield of genomic DNA varies depending on the white blood cell count of the patient blood sample, the extraction method, and the elution volume. However, DNA yield typically ranges between 16–135 µg/ml whole blood. Specifically, DNA yields from magnetic bead-based extraction on the QIAsymphony ranges between 16.9–51.7 µg/ml whole blood, from silica membrane-based extraction on the QIAcube 17.1–45.8 µg/ml whole blood, and from salting-out precipitation 4.9–53.7 µg/ml whole blood.

The PAXgene Blood DNA Tubes can be stored at –20ºC for up to 3 months. If tubes are to be kept at temperatures below –20ºC, freeze them first at –20ºC for 24 hours, then transfer them to –70ºC or –80ºC.
To freeze PAXgene Blood DNA Tubes, stand them upright in a wire rack or horizontally in a plastic bag. Do not freeze tubes upright in a styrofoam tray as this may cause the tubes to crack.

Yield of genomic DNA will vary depending on the cell count from the donor blood sample but average range is from 150 µg to 500 µg.

Yes, mitochondrial and genomic DNA are both isolated.

No. The volume of Buffer BG1 (lysis buffer) prefilled in the processing tubes, and all the other buffer volumes are optimized for use with blood from one PAXgene Blood DNA Tube.

To date, DNA purified from blood samples in PAXgene Blood DNA Tubes has been successfully tested for use in PCR, including multiplex PCR.

Blood collection using PAXgene Blood DNA Tubes is performed using standard phlebotomy protocol. Blood is collected into the evacuated tube using a needle or blood collection set with a standard needle holder that can accommodate a 13 mm diameter tube. Further information on phlebotomy technique with PAXgene Blood DNA Tubes can be found in the product handbook.

DNA purified using the PAXgene Blood DNA System should be dissolved in Buffer BG4 and stored at 2–8°C. For long-term storage, DNA can be stored at –20°C but multiple freezing and thawing cycles should be avoided.

PAXgene Blood DNA Tubes contain a proprietary EDTA formulation. Contents of the tube may cause irritation to eyes, respiratory system, and skin. For more information, please refer to the product handbook, download the MSDS, or contact BD Technical Service.

So far, we have shown that DNA purified using the PAXgene Blood DNA System can be used in PCR, including long range and multiplex PCR, restriction digestion and Southern analysis, as well as in various SNP detection procedures including,TaqMan®, LightCycler and Masscode technologies.

See trademarks. 

The system is designed for use only with human blood samples.

See subsequent FAQs for specific storage temperature and time information. Individual laboratories should validate shipping procedures.

The inside of the PAXgene Blood DNA Tube is sterilized during production.