Custom LNA Oligonucleotides
カスタムデザインが必要な実験のためのLNAオリゴヌクレオチド
Products
Custom LNA Oligonucleotidesは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。
Custom LNA Oligonucleotide Manual Design (25 nmol synthesis)
Cat. No. / ID: 339405
Custom LNA Oligonucleotide Manual Design, designed by QIAGEN's LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 25 nmol
Custom LNA Oligonucleotide Manual Design (100 nmol synthesis)
Cat. No. / ID: 339406
Custom LNA Oligonucleotide Manual Design, designed by QIAGEN's LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 100 nmol
Custom LNA Oligonucleotide Manual Design (250 nmol synthesis)
Cat. No. / ID: 339407
Custom LNA Oligonucleotide Manual Design, designed by QIAGEN's LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 250 nmol
Custom LNA Oligonucleotide Manual Design (1 µmol synthesis)
Cat. No. / ID: 339408
Custom LNA Oligonucleotide Manual Design, designed by QIAGEN's LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 1 µmol
Custom LNA Oligonucleotide Manual Design (5 µmol synthesis)
Cat. No. / ID: 339409
Custom LNA Oligonucleotide Manual Design, designed by QIAGEN's LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 5 µmol
Custom LNA Oligonucleotide Manual Design (10 µmol synthesis)
Cat. No. / ID: 339410
Custom LNA Oligonucleotide Manual Design, designed by QIAGEN's LNA experts to have optimal LNA content and positioning according to your needs; available with option of different purifications, labels or mixed bases; 10 µmol
Features
- 独自のLNAオリゴヌクレオチドをカスタマイズ
- ご自身での設計とQIAGENLNAエキスパートに設計依頼が可能
- 多様なラベルや修飾が選択可能
- 使いやすいオンラインデザインツール
Product Details
Custom LNA Oligonucleotidesは、短い配列または非常に類似した配列を含む研究に最適です。LNA技術を注ぎ込んだオリゴヌクレオチドの相補鎖に対する高い親和性は、伝統的なDNAまたはRNAオリゴと比較して、劇的な特異性と感度を向上させます。多くの場合、LNAオリゴヌクレオチドは、単一塩基のみ違う配列を見分けることができ、多くの実験を成功に導きます。
研究プロジェクトの見積もりや、専門チームとのご相談等、こちらまでお気軽にご連絡ください。
Custom LNA Oligonucleotideのラージスケールおよびマニュアルデザインに関しては、 こちらのフォームでお問合せいただくか、弊社サポートチームへメールでご連絡ください。
Performance
LNA oligonucleotides exhibit unprecedented thermal stability when hybridized to a complementary DNA or RNA strand. For each incorporated LNA monomer, the melting temperature (Tm) of the duplex increases by 2–8°C (see figure Replace DNA with LNA for higher melting temperature). In addition, LNA oligonucleotides can be made shorter than traditional DNA or RNA oligonucleotides and still retain a high Tm. This is important when the oligonucleotide is used to detect small or highly similar targets.
Since LNA oligonucleotides typically consist of a mixture of LNA and DNA or RNA, it is possible to optimize the sensitivity and specificity by varying the LNA content of the oligonucleotide. Incorporation of LNA into oligonucleotides has been shown to improve sensitivity and specificity for many hybridization-based technologies including PCR, microarrays and in situ hybridization (ISH).
Tm normalization enables robust detection, regardless of GC content. The Tm of a nucleotide duplex can be controlled by varying the LNA content. This feature can be used to normalize the Tm across a population of short sequences with varying GC content. For AT-rich nucleotides, which give low melting temperatures, more LNA is incorporated into the LNA oligonucleotide to raise the Tm of the duplex. This enables the design of LNA oligonucleotides with a narrow Tm range, which is beneficial in many research applications such as microarrays, PCR and other applications in which sensitive and specific binding to many different targets must occur under the same conditions simultaneously. The power of Tm normalization is demonstrated by the comparison of DNA and LNA probes for detection of miRNA targets with a range of CG content (see figure The power of Tm normalization).
Since LNA oligonucleotides typically consist of a mixture of LNA and DNA or RNA, it is possible to optimize the sensitivity and specificity by varying the LNA content of the oligonucleotide. Incorporation of LNA into oligonucleotides has been shown to improve sensitivity and specificity for many hybridization-based technologies including PCR, microarrays and in situ hybridization (ISH).
Tm normalization enables robust detection, regardless of GC content. The Tm of a nucleotide duplex can be controlled by varying the LNA content. This feature can be used to normalize the Tm across a population of short sequences with varying GC content. For AT-rich nucleotides, which give low melting temperatures, more LNA is incorporated into the LNA oligonucleotide to raise the Tm of the duplex. This enables the design of LNA oligonucleotides with a narrow Tm range, which is beneficial in many research applications such as microarrays, PCR and other applications in which sensitive and specific binding to many different targets must occur under the same conditions simultaneously. The power of Tm normalization is demonstrated by the comparison of DNA and LNA probes for detection of miRNA targets with a range of CG content (see figure The power of Tm normalization).
See figures
Principle
Use the guidelines below when designing your own Custom LNA Oligonucleotides:
- LNA will bind very tightly to other LNA residues. Avoid self-complementarity and cross-hybridization to other LNA-containing oligonucleotides
- Keep the GC content between 30–60%
- Avoid stretches of more than 4 LNA bases, except when very short (9–10 nucleotides) oligonucleotides are designed
- Avoid stretches of 3 or more Gs or Cs
- For novel applications, design guidelines may have to be established empirically
Find more information about the LNA technology here and how to design LNA oligonucleotides here.
Applications
LNAオリゴヌクレオチドは、以下を含む様々な用途で使用することができます：
- miRNA研究
- Small RNA研究
- SNPジェノタイピング
- mRNAアンチセンスオリゴヌクレオチド
- アレル特異的PCR
- RNAi
- DNAザイム
- 蛍光プローブ
- モレキュラービーコン
- マイクロアレイによる遺伝発現解析
- 遺伝子修復・エクソン・スキップ
- スプライスバリアント検出
- 比較ゲノムハイブリダイゼーション（CGH）
Supporting data and figures
Replace DNA with LNA for higher melting temperature.
On the left, progressive substitutions of DNA nucleotides with LNA increases the melting temperature of the oligonucleotide, while maintaining the recognition sequence and specificity of the probe. On the right, LNA substitutions allow shortening of the probe, while maintaining the same Tm.
Resources
MSDS (1)
Safety Data Sheets (13)
Certificates of Analysis (1)