Qproteome Mammalian Protein Prep Kit
For total protein preparations from mammalian cells
The kit contains a lysis buffer for total protein preparations from mammalian cells for use in proteomics procedures.
The Qproteome Mammalian Protein Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Performance
The Qproteome Mammalian Protein Prep Kit enables efficient extraction of total protein from cultured mammalian cells (see figure "Efficient extraction of proteins from cultured cells"). The efficiency of extraction is demonstrated in the table below:
Principle
The Qproteome Mammalian Protein Prep Kit provides gentle but efficient detergent-based lysis of mammalian cells to deliver a soluble protein fraction suitable for any downstream application. Cell pellets are incubated with Mammalian Cell Lysis Buffer supplemented with Benzonase — an enzyme that digests nucleic acids — and a protease inhibitor, which protects the released proteins from enzymatic degradation. No specialized equipment is required.
Procedure
Cells are simply incubated with lysis buffer and centrifuged to separate cell debris. The supernatant contains the soluble protein fraction, which can be further purified or directly analyzed, for example by SDS-PAGE.
Applications
The Qproteome Mammalian Protein Prep Kit is used for total protein preparations from mammalian cells for proteomics procedures (e.g., 2D-PAGE, mass spectrometry).
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