MinElute Gel Extraction Kit
For gel extraction of up to 5 μg DNA fragments (70 bp to 4 kb) in low elution volumes
- Very small elution volumes
- Fast procedure and easy handling
- High, reproducible recoveries
- Gel loading dye for convenient sample analysis
The MinElute Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments of 70 bp – 4 kb from up to 400 mg gel slices. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering high yields of highly concentrated DNA. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. DNA fragments purified with the MinElute system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription. The MinElute Gel Extraction Kit can be automated on the QIAcube Connect.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.
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MinElute Gel Extraction Kit (50)
50 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
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28604
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MinElute Gel Extraction Kit (250)
250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
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28606
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MinElute Gel Extraction Kit は分子生物学実験用です。疾病の診断、治療または予防の目的には使用することはできません。
MinElute操作手順|高濃度なDNA精製|GelPilot Loading Dye|MinEluteメンブレン構造|pH 指示薬|Spin Column操作オプション - D|Spin Column操作オプション - E|Spin Column操作オプション - C|Spin Column操作オプション - B|Spin Column操作オプション - A|
結合―洗浄―溶出の簡単な操作 |MinElute Gel Extraction Kit および3種類のシリカベースの他社DNA精製キット(メーカーを表記)を用いて精製した500 bpと1000 bpフラグメントのアガロースゲル解析。各溶出液から2 µlを1.5%アガロースゲルにロードした。M:マーカー。|GelPilot Loading Dye に入っている3種類のマーカー色素によりDNA電気泳動の至適化が容易。 |ユニークなメンブレン構造を持つMinElute Spin Column断面図(utility model pending)。|溶解および結合バッファー中のpH指示薬により、DNA吸着の至適pH(pH <7.5)かどうかを簡単に確認できる。アガロースゲル電気泳動バッファーを繰り返し使用したり、間違って調製した場合に、結合溶液のpHは至適pHより高くなる。このような場合は、3M 酢酸ナトリウム、pH 5.0 を10 µl 添加してpHを簡単に調整することが可能。|QIAvac 24 Plus|QIAcube |ルアーコネクター付きマニホールド |QIAvac 24 |マイクロ遠心機 |
パフォーマンス
The MinElute Gel Extraction Kit removes nucleotides, enzymes, salts, agarose, ethidium bromide, and other impurities from DNA samples, delivering highly concentrated DNA suitable for a variety of downstream applications (see figure "Higher DNA concentrations").
The MinElute QIAquick Gel Extraction Kit provides spin columns for gel extraction. Using a microcentrifuge or vacuum manifold, high concentration DNA of 70 bp – 4 kb is quickly purified. (DNA fragments from 4 kb – 10 kb should be purified using the QIAquick Gel Extraction Kit and DNA fragments smaller than 70 bp or larger than 10 kb should be extracted with the QIAEX II Gel Extraction System.)
原理
MinElute Kits contain a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.
Gel loading dye
To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure "GelPilot Loading Dye").
操作手順
The MinElute system uses a simple bind-wash-elute procedure (see flowchart "MinElute procedure"). Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding (see figure "pH Indicator Dye"), and the mixture is applied to the MinElute spin column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Handling
MinElute spin columns are designed to provide two convenient handling options (see "MinElute procedure"). The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as the QIAvac 24 Plus with QIAvac Luer Adapters. The MinElute Gel Extraction Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results (see figures "Spin column handling options A, B, C, D, and E").
アプリケーション
DNA fragments purified with the MinElute or QIAquick System are ready for direct use in all applications, including:
- Sequencing, including next generation sequencing
- Microarray analysis
- Ligation and transformation
- Restriction digestion
- Labeling
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Feature
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Specifications
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Binding capacity
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5 µg
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Elution volume
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10 µl
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Format
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Tube
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Fragment size
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70 bp – 4 kb
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Processing
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Manual
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Recovery: oligonucleotides dsDNA
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Recovery: dsDNA fragments
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Sample type: applications
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DNA: PCR reactions
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Technology
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Gel extraction
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FAQ ID -120
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FAQ ID -133
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FAQ ID -148
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FAQ ID -205
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FAQ ID -313
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I received one of the kits in the list below containing the MinElute columns, however they were left out for a while and not stored at 2-8°C upon receipt. Can I still use them? AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro
FAQ ID - 3560
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FAQ ID -581
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FAQ ID -759
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FAQ ID -199
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FAQ ID -2460
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画像
MinElute操作手順
結合―洗浄―溶出の簡単な操作
高濃度なDNA精製
MinElute Gel Extraction Kit および3種類のシリカベースの他社DNA精製キット(メーカーを表記)を用いて精製した500 bpと1000 bpフラグメントのアガロースゲル解析。各溶出液から2 µlを1.5%アガロースゲルにロードした。M:マーカー。
GelPilot Loading Dye
GelPilot Loading Dye に入っている3種類のマーカー色素によりDNA電気泳動の至適化が容易。
MinEluteメンブレン構造
ユニークなメンブレン構造を持つMinElute Spin Column断面図(utility model pending)。
pH 指示薬
溶解および結合バッファー中のpH指示薬により、DNA吸着の至適pH(pH <7.5)かどうかを簡単に確認できる。アガロースゲル電気泳動バッファーを繰り返し使用したり、間違って調製した場合に、結合溶液のpHは至適pHより高くなる。このような場合は、3M 酢酸ナトリウム、pH 5.0 を10 µl 添加してpHを簡単に調整することが可能。
Spin Column操作オプション - D
QIAvac 24 Plus
Spin Column操作オプション - E
QIAcube
Spin Column操作オプション - C
ルアーコネクター付きマニホールド
Spin Column操作オプション - B
QIAvac 24
Spin Column操作オプション - A
マイクロ遠心機
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