Investigator HDplex Kit
For multiplex amplification of 12 non-standard STR loci to give additional discriminatory power
The Investigator HDplex Kit amplifies a set of STR markers, most of which are not featured in commonly used marker standards. This enables the reliable differentiation of forensic samples derived from related individuals and gives heightened discriminatory power in these demanding cases. The genetic analysis is calibrated with BT5 Matrix, an optimized dye technology.
The Investigator HDplex Kit has been validated using the GeneAmp 9700 thermal cycler (in standard mode), and the ABI PRISM 310, ABI PRISM 3100, and Applied Biosystems 3130 Genetic Analyzers.
The recommended amount of DNA under standard conditions is 0.35 ng. Internal validations demonstrated robust and balanced results with 0.2–1.0 ng DNA, and reliable results with <0.1 ng DNA.
The Investigator HDplex Kit enables the simultaneous amplification of 13 STR markers (D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D21S2055, SE33, and the gender-specific Amelogenin). These markers include some highly polymorphic STR markers that are not included in commonly used standards enabling higher discrimination in difficult cases.
The twelve polymorphic STR markers and Amelogenin are simultaneously amplified and labeled with the dyes listed in the table "Investigator HDplex Kit dyes and corresponding markers".
The amplified loci undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as QIAGEN's Investigator IDproof or IDproof Mixture Software. See the table "Technical specifications" for more information.
The Investigator HDplex Kit enables reliable generation of DNA profiles from blood, buccal swabs, and forensic stains. Thanks to the STR markers used, this kit is highly suited for:
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