Nucleic acid separation on the QIAxcel Advanced System, using the QIAxcel DNA High Resolution Kit takes less than 10% of the time of conventional high-resolution agarose.
The indicated amounts of DNA extracted from a lymphoma related cell line (Ramos) were spiked into human leukocyte DNA and the mutated Ramos target was detected together with 2 internal controls. Using the Type-it Mutation Detect PCR Kit, the mutated gene was detected even when only 25 pg of DNA was present. [A] Electrophoresis was performed on a 1.3% agarose gel. [B] Electrophoresis was performed on the QIAxcel Advanced System using the QIAxcel High Resolution Kit. M: 100 bp ladder.
PCR products were generated using the QIAGEN Multiplex PCR Kit according to the standard protocol. PCR samples (13 µl) were analyzed [A] on a 2% agarose gel and [B] and [C] using the QIAxcel system with the QIAxcel DNA High Resolution gel cartridge and the preinstalled OM500 method. [B] The gel image produced by the QIAxcel system shows much higher resolution than the agarose gel. [C] Each sample lane can be visualized individually in electropherogram form. Lane 7 is shown.
PCR products from a standard PCR were directly analyzed using the QIAxcel DNA Fast Analysis Kit without prior purification.
Nucleic acid molecules are separated according to size in gel-filled capillaries.