QIAsymphony SP for FFPE sample preparation

For automated, high throughput DNA prep from FFPE tissue samples

Rapid purification of high-quality, ready-to-use DNA for use in research applications
Enzymatic removal of cytosine deamination artifacts for FFPE samples
Automation on the QIAsymphony Sample Preparation
Outstanding results in DNA sequencing applications

With the QIAsymphony DSP DNA Mini Kit, in combination with the QIAsymphony GeneRead DNA FFPE Treatment Kit and QIAsymphony SP, it is possible to fully automate DNA extraction from up to 96 FFPE samples in parallel. Enzymatic removal of cytosine deamination artifacts from FFPE samples in combination with optimized protocols provide high yields of pure DNA, suitable for downstream sequencing applications in the GeneReader NGS System workflow. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.

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Cat No./ID: 185306

QIAsymphony GeneRead DNA FFPE Treatment Kit (192)

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For uracil-N-glycosylase treatment of FFPE tissue specimens using the QIAsymphony SP Tissue_LC_200_V7_DSP protocol

Cat No./ID: 9001297

QIAsymphony SP

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QIAsymphony sample prep module: includes 1-year warranty on parts and labor

Cat No./ID: 937236

QIAsymphony DSP DNA Mini Kit (192)

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For 192 preps of 200 µl each: Includes 2 reagent cartridges and enzyme racks and accessories.

In the context of the GeneReader NGS System, the QIAsymphony SP, QIAsymphony DPS DNA Mini Kit and QIAsymphony GeneRead FFPE Treatment Kits are intended for Research Use Only. Not for use in diagnostic procedures.

Product Details

Prefilled, sealed reagent cartridges.

QIAsymphony DSP DNA procedure.

Schematic of the QIAsymphony SP principle.

The QIAsymphony SP processes a sample containing magnetic particles as follows: A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod cover is positioned above another well and the magnetic particles are released. The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process up to 24 samples simultaneously. Steps 1 and 2 are repeated several times during sample processing.

Principle

The QIAsymphony DSP DNA mini kit in combination with the QIAsymphony GeneRead DNA FFPE Treatment Kit can be used to purify DNA from FFPE tissues. This combines the speed and efficiency of silica-based purification of DNA with the convenient handling of magnetic particles (see figure "Schematic of the QIAsymphony SP principle"). Innovative, ready-to-run reagent cartridges are prefilled with all reagents required for the purification procedure and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure "Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves you valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.

Procedure

The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute (see figure "QIAsymphony DSP DNA procedure"). Optimized protocols provide high quality nucleic acids with efficient removal of proteins, nucleases, and other impurities. Nucleic acids purified with QIAsymphony GeneRead DNA FFPE Treatment Kit and QIAsymphony DSP DNA Mini Kit is ready for direct use in downstream library preparation and sequencing applications on the GeneReader NGS System. The user can choose between different elution volumes.

Applications

Designed for use in the GeneReader NGS System workflow.

Product Resources

Brochures & Guides (6)

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	(EN) - QIAsymphony RGQ — Pure Performance	Show details
	QIAsymphony SP/AS HID Brochure – In Tune with Your DNA Evidence	Show details
	QIAGEN Paternity Testing Solutions (eBook)	View
	QIAsymphony SP/AS – Flexible Input, Quality Output (eBook)	View
	Forensic Casework Automation Workflows – Digital Product Profile Explore our new, easy-to-navigate digital Product Profile!	Show details
	Advanced Workflow Solutions for Confidence in Your Evidence (eBook)	View

FAQs (71)

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	Is there a charge for user training on the QIAsymphony SP System? FAQ ID -1900	View
	What are the dimensions and weight of the QIAsymphony SP System? FAQ ID -1901	View
	Is it possible to modify QIAsymphony protocols? FAQ ID -1903	View
	Can I program my own protocols for the QIAsymphony SP? FAQ ID -1904	View
	How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle? FAQ ID -1905	View
	Can uneven sample numbers be processed on the QIAsymphony SP module? FAQ ID -1906	View
	What sample input volumes can be used on the QIAsymphony SP? FAQ ID -1907	View
	What is the range of elution volumes on the QIAsymphony SP? FAQ ID -1908	View
	Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)? FAQ ID -1909	View
	Does the QIAsymphony SP system generate run reports and/or log files? FAQ ID -1911	View
	Can protocol runs on the QIAsymphony SP be interrupted and resumed? FAQ ID -1912	View
	Are there any specific cleaning solutions recommended for the QIAsymphony SP? FAQ ID -1913	View
	How can the QIAsymphony SP system be decontaminated? FAQ ID -1914	View
	What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP? FAQ ID -1915	View
	Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction? FAQ ID -1916	View
	How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP? FAQ ID -1917	View
	What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP? FAQ ID -1918	View
	What are the expected DNA yields from tissue processed on the QIAsymphony SP? FAQ ID -1919	View
	Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols? FAQ ID -1920	View
	Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP? FAQ ID -1921	View
	Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP? FAQ ID -1937	View
	Is sample pretreatment required for purifying RNA on the QIAsymphony SP? FAQ ID -1938	View
	What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP? FAQ ID -1939	View
	How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP? FAQ ID -1940	View
	How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation? FAQ ID -1941	View
	What are the expected yields of total RNA isolated with the QIAsymphony SP? FAQ ID -1942	View
	What RNA elution volumes are available with the QIAsymphony RNA Kit? FAQ ID -1943	View
	Can the QIAsymphony RNA Kit be used to purify RNA from blood? FAQ ID -1945	View
	Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument? FAQ ID -2027	View
	What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP? FAQ ID -2028	View
	What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit? FAQ ID -2029	View
	Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP? FAQ ID -2030	View
	Should sample lysate volumes be exactly 200 µl, 500 µl, or 1000 µl when loaded on the QIAsymphony SP? FAQ ID -2031	View
	Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL? FAQ ID -2032	View
	Do all QIAsymphony DNA Investigator protocols use carrier RNA? FAQ ID -2033	View
	What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit? FAQ ID -2034	View
	Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)? FAQ ID -2035	View
	What sample types can be processed using the QIAsymphony Virus/Bacteria protocols? FAQ ID -2036	View
	What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits? FAQ ID -2037	View
	Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol? FAQ ID -2038	View
	Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument? FAQ ID -2039	View
	Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run? FAQ ID -2040	View
	Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol? FAQ ID -2154	View
	What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2156	View
	How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2157	View
	What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2158	View
	How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol? FAQ ID -2159	View
	Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol? FAQ ID -2160	View
	Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol? FAQ ID -2162	View
	Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol? FAQ ID -2163	View
	How should DNA isolated on the QIAsymphony be stored? FAQ ID -2165	View
	How does magnetic-particle carry-over in QIAsymphony eluates need to be handled? FAQ ID -2166	View
	What sample types can be processed using the tissue protocols and the QIAsymphony DSP DNA Mini Kit? FAQ ID -2925	View
	Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction? FAQ ID -2926	View
	How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP? FAQ ID -2927	View
	What sample types can be processed using the QIAsymphony DSP Virus/Pathogen protocols? FAQ ID -2928	View
	Why do I have to use an ACS (Assay Control Set)? FAQ ID -2933	View
	What sample types should be processed with the low content (LC) and high content (HC) protocols for DNA extraction on the QIAsymphony SP? FAQ ID -2989	View
	What are the expected DNA yields from tissue processed on the QIAsymphony SP? FAQ ID -2990	View
	Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP? FAQ ID -2991	View
	What sample volumes can be used with the tissue HC and LC protocols on the QIAsymphony SP? FAQ ID -2992	View
	What are the recommended lysis times for tissue samples on the QIAsymphony SP? FAQ ID -2993	View
	Is clot detection possible on the QIAsymphony? FAQ - ID 3588	View
	In which tubes is clot detection possible on QIAsymphony? FAQ ID - 3589	View
	How can I determine if a tube is suitable for clot detection on QIAsymphony? FAQ ID - 3590	View
	How can I create a report/logfile on my QIAsymphony SP system? FAQ ID - 3594	View
	Can I change the language on my QIAsymphony SP system? FAQ ID - 3595	View
	Which barcodes on sample tubes are accepted by the QIAsymphony SP? FAQ ID - 3598	View
	What is the thermal output for a QIAsymphony SP and AS instrument? FAQ ID -3057	View
	What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced? FAQ ID -3055	View
	Is the QIAsymphony RNA Kit compatible with stabilized samples? FAQ ID -1944	View

Scientific Posters (4)

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	(EN) - Development of a new platform for fully automated purification of nucleic acids from a broad spectrum of forensic specimens	Show details
	(EN) - Application of a HT magnetic bead-based DNA extraction system to diverse mAb process intermediates	Show details
	(EN) - Processing challenging casework samples — new protocols for the QIAsymphony SP/AS	Show details
	(EN) - Automated DNA extraction from FFPE tissue using a xylene-free deparaffinization method on QIAsymphony	Show details

Protocol Files (8)

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	(EN) - QIAsymphony V4 SP Extended Applications DSP HPV Media For use with the QIAsymphony SP instrument (software version 4.0)	Show details
	(EN) - QIAsymphony V4 AS Certal Protocols For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)	Show details
	QIAsymphony V5 SP Applications For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)	Show details
	(EN) - QIAsymphony V4 AS Installation Protocols For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)	Show details
	(EN) - QIAsymphony V4 AS QIAGEN PCR Applications For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)	Show details
	(EN) - QIAsymphony V4 AS mericon Protocols For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)	Show details
	QIAsymphony SP Applications Investigator V5 For use with the QIAsymphony SP/AS instruments (software version 5.0 or higher)	Show details
	QIAsymphony SP PowerFecal Pro DNA Stool/Soil V1 For use with the QIAsymphony SP instruments (software version 4.0 or higher)	Show details

Validation Reports (2)

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	(EN) - QIAGEN Validation Report — Developmental validation of the forensic QIAsymphony workflow	Show details
	Validation Certificate QIAsymphony SP/AS	Show details

Application Notes (3)

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	Improving workflow efficiency with the QIAsymphony SP Instrument and the Investigator Lyse&Spin Basket Kit	Show details
	QIAsymphony SP – Bode Cellmark Forensics Application Note High-throughput DNA sample prep using the QIAsymphony SP instrument: An overview of workflow optimization at Bode Cellmark Forensics.	Show details
	(EN) - Evaluation of QIAGEN’s universal next generation sequencing workflow and SNP panel including the Ion PGM system for identity and relationship testing	Show details

Application/Protocol Documents (27)

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	(EN) - Certal Residual RGQ-5-plex-HRM RD72 V2 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - Certal Residual RGQ-5-plex-HRM RS4 V2 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Cronobacter spp QIAGEN-PCR-96 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Cronobacter spp QS-RGQ RD72 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Cronobacter spp QS-RGQ RS V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Listeria spp QIAGEN-PCR-96 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Campylobacter spp QIAGEN-PCR-96 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Campylobacter spp QS-RGQ RD72 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Campylobacter spp QS-RGQ RS V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Listeria spp QS-RGQ RD72 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Listeria spp QS-RGQ RS V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Campylobacter triple QIAGEN-PCR-96 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Campylobacter triple QS-RGQ RD72 V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Campylobacter triple QS-RGQ RS V1 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon L. monocytogenes QIAGEN-PCR-96 V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon L. monocytogenes QS-RGQ RD72 V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon L. monocytogenes QS-RGQ RS V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Salmonella spp QIAGEN-PCR-96 V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Salmonella spp QS-RGQ RD72 V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - mericon Salmonella spp QS-RGQ RS V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - QIAGEN-PCR-96 (15+10) 1ch V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - QIAGEN-PCR-96 (40+10) 4ch V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - QIAGEN-PCR-96 (45+5) 4ch V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - QIAGEN-PCR-LC (15+5) 1ch V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - QIAGEN-PCR-RGQ (15+10) 1ch V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - QIAGEN-PCR-RGQ (20+5) 1ch V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details
	(EN) - QIAGEN-PCR-RGQ RD72 (20+5) 1ch V3 For use with the QIAsymphony AS (software version 4.0 or higher)	Show details

Instrument Technical Documents (10)

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	Differences between QIAsymphony software versions 4.0.3/4.1 and 5.0.3	Show details
	QIAsymphony File Transfer User Guide For use with QIAsymphony SP/AS instruments (software version 5.0)	Show details
	QIAsymphony SP Recovery Procedure for DNA Investigator Applications (software version 4.0 or higher and HID 1.0) QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0	Show details
	QIAsymphony SP Recovery Procedure for DNA Investigator ADV (Advanced) Applications (software version 4.0 or higher and HID 1.0) QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0	Show details
	QIAsymphony SP Recovery Procedure for DNA Investigator ADV (Advanced) HE (High Efficiency) Applications (software version 4.0 or higher and HID 1.0) QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0	Show details
	QIAsymphony SP Recovery Procedure for DNA Investigator HE (High Efficiency) Applications (software version 4.0 or higher and HID 1.0) QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0	Show details
	(EN) - QIAsymphony Cabinet SP/AS User Guide QIAsymphony Cabinet SP/AS document	Show details
	(EN) - QIAsymphony Cabinet SP/AS Specifications QIAsymphony Cabinet SP/AS documents	Show details
	(EN) - QIAsymphony File Transfer User Guide Software document for QIAsymphony software version 4.0.1	Show details
	(EN) - Differences between QIAsymphony software versions 4.0.2 and 4.0.3 Differences between QIAsymphony software versions	Show details

Supporting Software and Tools (2)

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	Work List Tool For QIAsymphony Software version 5.0	Show details
	QIAsymphony Management Console For QIAsymphony software version 5.0.4	Show details

Labware Files and Documents (1)

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	(EN) - QIAsymphony SP/AS adapters and tube inserts	Show details

Instrument User Manuals (5)

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	QIAsymphony SP/AS Consolidated Operating Guide For use with QIAsymphony SP/AS instruments (software version 5.0)	Show details
	QIAsymphony SP/AS General Description For use with QIAsymphony SP/AS instruments (software version 5.0)	Show details
	QIAsymphony SP/AS User Manual - Operating the QIAsymphony SP For use with QIAsymphony SP/AS instruments (software version 5.0)	Show details
	QIAsymphony SP/AS User Manual - Operating the QIAsymphony AS For use with QIAsymphony SP/AS instruments (software version 5.0)	Show details
	QIAsymphony Management Console User Manual For use with QIAsymphony SP/AS instruments (software version 5.0)	Show details

Performance Data (1)

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	(EN) - QIAsymphony DSP DNA Performance Data	Show details

Kit Handbooks (2)

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	QIAsymphony DSP DNA Handbook	Show details
	QS GeneRead DNA FFPE Treatment Kit Handbook	Show details

Labware Documents (1)

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	Labware List — QIAsymphony DSP DNA Kits (SOW-516-7) For use with software version 4.0 or higher	Show details

Application/Protocol Documents for IVD Use (7)

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	QIAsymphony SP Protocol Sheet Tissue_LC_200_V7_DSP and Tissue_HC_200_V7_DSP protocol For use with software version 4.0 or higher	Show details
	QIAsymphony SP Protocol Sheet DNA_Blood_200_V7_DSP protocol For use with software version 4.0 or higher	Show details
	QIAsymphony SP Protocol Sheet DNA_Blood_400_V6_DSP protocol For use with software version 4.0 or higher	Show details
	QIAsymphony SP Protocol Sheet DNA_Buffy_Coat_400_V6_DSP protocol For use with software version 4.0 or higher	Show details
	QIAsymphony SP Protocol Sheet VirusBlood200_V5_DSP protocol For use with software version 4.0 or higher	Show details
	QIAsymphony SP Protocol Sheet DNA_Blood_1000_V7_DSP protocol For use with software version 4.0 or higher	Show details
	QIAsymphony SP Protocol Sheet DNA_Buffy_Coat_200_V7 DSP protocol For use with software version 4.0 or higher	Show details

Application/Protocol Documents for User-Validated Use (1)

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	QIAsymphony SP Protocol Sheet Tissue_LC_200_V7_DSP and Tissue_HC_200_V7_DSP protocols (user-validated) For use with software version 4.0 or higher	Show details

Safety Data Sheets (3)

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	MSDS QIAsymphony SP
	MSDS QIAsymphony DSP DNA Mini Kit (192)
	MSDS QIAsymphony GeneRead DNA FFPE Treatment Kit (192)