Genomic DNA and total RNA were purified from 10-fold serial dilutions of rat kidney homogenate using the AllPrep DNA/RNA Micro Kit; at each dilution, DNA was purified from duplicates, and RNA was purified from 4 replicates. [A] Real-time PCR analysis of the Pgk1 gene and [B] real-time RT-PCR analysis of the Jun transcript showed reliable quantification over a wide dynamic range, from 5 mg down to 50 ng tissue.
Total RNA was purified from 1 x 106 Jurkat cells using the AllPrep DNA/RNA Mini Kit and analyzed on the Agilent 2100 Bioanalyzer. The RIN value was 10, indicating intact RNA. M: marker; 18S: 18S rRNA; 28S: 28S rRNA.
Total RNA and genomic DNA were purified from 10 mg samples of rat liver using AllPrep technology (the AllPrep DNA/RNA Mini Kit). [A] Purified RNA samples were analyzed on a 1.2% formaldehyde agarose gel. [B] Purified DNA samples were analyzed on a 0.8% agarose gel. M: markers.
Genomic DNA was purified from mouse tissues using the AllPrep DNA/RNA Mini Kit. Multiplex PCR of 8 targets (192–1953 bp; GC content in the range of 19–61%) was performed using 100 ng DNA per sample. M: markers; NTC: no template control; Li: liver; K: kidney; Lu: lung.