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EZ1 ccfDNA Kits

For isolation of free-circulating DNA from human plasma or serum

Features

  • Efficient recovery of fragmented DNA
  • Scalable sample input volume of 1–10 ml
  • No organic extraction or ethanol precipitation
  • Removal of contaminants and inhibitors
  • Semi-automated, convenient and streamlined protocol
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EZ1 ccfDNA Midi Kit (48)

Cat. No. / ID: 954154

For 48 preps (4 or 5 ml sample input volume each): 48 reagent cartridges (EZ1 ccfDNA), QIAGEN Proteinase K, Magnetic Bead Suspension, Buffers, Bead Elution Tubes, Disposable Tip Holders, Disposable Filter-Tips, Elution Tubes (1.5 ml)
KitCardAccessoriesInstrument
EZ1 ccfDNA Kit
EZ1 Advanced XL ccfDNA Card
Accessories
EZ1 Advanced XL
Sample volume
5–10 ml
1–4 ml
EZ1 ccfDNA Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

EZ1 ccfDNA Kits provide semi-automated concentration and purification of circulating cell-free DNA from plasma or serum using the EZ1 Advanced XL Instrument.

Performance

EZ1 ccfDNA Kits enable efficient purification of circulating DNAs from human plasma or serum. Upfront manual pre-concentration of ccfDNA on magnetic beads allows flexible and scalable handling of volumes from 1 to 10 ml. Subsequent ccfDNA cleanup on the EZ1 Advanced XL instrument provides convenient automated bead-based purification of up to 14 samples in parallel.
Free-circulating DNA is ready for use in downstream reactions or storage at –30 to –15°C. Purified nucleic acids are free of proteins, nucleases and other impurities.
The EZ1 ccfDNA Mini Kit has been optimized for sample volumes from 1 to 4 ml while the EZ1 ccfDNA Midi Kit has been optimized for sample volumes from 4 to 10 ml. Yield depends on the sample volume and the concentration of circulating nucleic acids in the sample (typically 1–100 ng/ml in plasma).

Principle

Free-circulating DNA, such as tumor-specific extracellular DNA fragments in the blood or fetal DNA in maternal blood, are present in serum or plasma usually as short fragments of <1000 bp.
EZ1 ccfDNA Kits enable efficient purification of these circulating DNAs from human plasma or serum.

Procedure

EZ1 ccfDNA Kits use a fast procedure involving manually carried out pre-concentration of circulating nucleic acids onto magnetic beads and cleanup of the resulting pre-eluate on the EZ1 Advanced XL Instrument, all in a quick and convenient automated procedure.
The magnetic-particle technology combines the speed and efficiency of silica-based nucleic acid purification with the convenient handling of magnetic particles. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples.
The simple procedure, which is suited for simultaneous processing of multiple samples, provides pure nucleic acids in less than 1 hour for 14 samples.

Applications

EZ1 ccfDNA Kits provide semi-automated concentration and purification of circulating cell-free DNA from plasma or serum using the EZ1 Advanced XL Instrument.

Supporting data and figures

Resources

Kit Handbooks (2)
for separation of BioMag® particles in 15 ml or 50 ml tubes
Safety Data Sheets (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.

FAQ

What is the Globally Harmonized System of Classification and Labelling of Chemicals (GHS) and what is the impact for me as a customer?
For overview and further information on the Globally Harmonized System of Classification and Labelling of Chemicals (GHS) please visit www.qiagen.com/ghs .
FAQ ID - 3428
What type of magnet do you recommend for handling the magnetic beads in the Qproteome Plasma Membrane Protein protocol?

Since the Qproteome Plasma Membrane Protein procedure requires 1.5 ml/2.0 ml reaction tubes, we recommend using the follwing magnets for handling the magnetic beads:

Alternatively, comparable tube magnets or a standard clip-board magnet should also work.

FAQ ID -1193
Why have the hazard symbols accompanying products changed?
The Globally Harmonized System (GHS) is an initiative by the United Nations aiming to harmonize chemical hazard communication worldwide. Hazards symbols accompanying products are changing to those of the GHS. Information on the adoption of this new system is available on our GHS Hazard Labeling pages.
FAQ ID - 3430