Selection Guide

Simplify kit selection with our helpful selection guides

Not sure which product to choose? Our helpful selection guides make decision making a lot easier! Choose from the following:

Small-scale plasmid purification — 96-well format
DNA
grade
Transfection
grade
Molecular
biology
grade
Sequencing grade
Speed Standard Fast Fast Standard
Kit name QIAGEN
Plasmid Plus
96 Miniprep
and BioRobot 
Kits
QIAprep 96
Plus Miniprep
and BioRobot
Kits
QIAprep 96
Turbo Miniprep
and BioRobot
Kits
 R.E.A.L.
Prep 96
Plasmid Kit
Binding
capacity
<50 µg <50 µg <20 µg <30 µg
Culture
volume
(3–4 x 109
cells/ml)
1.25 –5 ml 1.25 –5 ml 1–5 ml 2.5 ml BAC/PAC/P1,
1.3 ml plasmid,
1.3–2.5 ml cosmid
Yield 15–50 µg 15–50 µg 4–20 µg 1 µg BAC/PAC/P1
Elution
volume
80 µl (100–120
µl for a 5 ml
culture)
80 µl (100–120
µl for a 5 ml
culture)
100 µl 22 µl BAC/PAC/P1,
50–250 µl plasmid,
50 µl cosmid
Procedure QIAvac 96,
centrifuge,
BioRobot
Universal
System
QIAvac 96,
centrifuge,
BioRobot
Universal
System
QIAvac 96,
centrifuge,
BioRobot
Universal
System
QIAvac 96,
centrifuge
Time 50 min 40 min 45 min 70 min
Small-scale plasmid purification kits
DNA grade Transfection
grade
Molecular
biology grade
Sequencing
grade
Speed Fast Fast Ultrafast
Kit name QIAGEN
Plasmid Mini Kit
QIAprep Spin
Miniprep Kit
Quicklyse
Miniprep Kit
Binding
capacity
<20 µg <50 µg <15 µg
Culture
volume
(3–4 x 109
cells/ml)
3 ml 1–5 ml 1–3 ml
Yield 20 µg 5–50 µg 8–15 µg
Elution volume Variable,
50 µl recommended
50 µl 50 µl
Procedure Centrifuge,
QIAcube
QIAvac 24 Plus,
centrifuge,
QIAcube
Centrifuge
Time 80 min 30 min 9 min
Large-scale plasmid purification kits

DNA grade

  Endotoxin-free Transfection grade Molecular biology grade
Speed Standard Standard Fast
Ultrafast Ultrafast Standard Ultrafast
Kit name   EndoFree
Plasmid Kits
QIAGEN Plasmid Kits QIAfilter
Plasmid Kits
QIAGEN
Plasmid Plus Kits
HiSpeed
Plasmid Kits
QIAGEN
Large-Construct
Kit
CompactPrep
Plasmid Kits
Culture volume
(3–4 x 109
cells/ml)
Midi   _
 25 ml, 100 ml
25 ml,
50–100 ml
20–25 ml,
25–35 ml (hy)
,
50 ml
50 ml,
150 ml
500 ml 25 ml,
50 ml
Maxi 100 ml, 250 ml
 100 ml, 500 ml
100 ml,
250 ml
80–100 ml,
100–130 ml (hy)
,
200 ml
150 ml,
250 ml
 100 ml,
250 ml
Mega 500 ml/1.5 g,
2.5 l / 7.5g
500 ml/1.5 g, 2.5 l/ 7.5g
500 ml/1.5 g,
2.5 l/7.5g
500 ml
500 ml only
rec for hc
Giga 2.5 l/7.5 g,
not rec. for lc
2.5 l/7.5 g, 5 l/15 g
2.5 l/7.5 g,
not rec. for lc
2.5 l
2.5 l only
rec for hc
Yield Midi  –
75–100 µg, 20–100 µg
75–100 µg,
20–100 µg
100–200 µg,
150–250 µg (hy)
,
30–100 µg
100–200 µg,
30–100 µg
Up to 50 µg
BAC/PAC/P1 DNA,
up to 200 µg
cosmid DNA 
100–200 µg,
30–100 µg
Maxi 300–500 µg,
50–250 µg
100–500 µg, 20–250 µg
300–500 µg,
50–250 µg
750 µg, 1 mg
(hy)
,
50–250 µg
300–750 µg,
50–250 µg
350–750 µg,
50–250 µg
Mega 1.5–2.5 mg,
0.5–2.5 mg
1.5–2.5 mg, 0.5–2.5 mg
1.5–2.5 mg,
0.5–2.5 mg
Up to 2.5 mg
1.5–2.5 mg,
endotoxin-free
<0.1 EU/µg DNA
Giga 7.5–10 mg
7.5–10 mg, 1–5 mg
7.5–10 mg
Up to 10 mg
7–10 mg,
endotoxin-free
<0.1 EU/µg DNA
Time Midi  –
150 min
110 min
20 min
<45 min
280 min <20 min
Maxi 150 min
160 min
120 min
20 min
<60 min
<20 min
Mega 220 min
220 min
190 min
40 min
50 min
Giga 310 min
320 min
280 min
50 min
50 min
Procedure Lysate clearing
by precipitator
or vacuum,
gravity-flow
Gravity-flow Lysate clearing
by precipitator or
vacuum,
gravity-flow
Vacuum and
centriguge
Gravity-flow
and precipitator
Centrifuge Centrifuge
and vacuum
X
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