Gene expression analysis

Gene expression profiling simultaneously compares the expression levels of multiple genes between two or more samples. This analysis can help scientists establish the molecular basis of phenotypic differences and select gene targets for in-depth study. Gene expression profiling provides valuable insight into the role of differential gene expression in normal biological states and diseases.

Benefits of nanoplate dPCR for gene expression quantification

  • Detect small-fold changes, especially in low-template amounts
  • Validate results with an absolute concentration and abundance below 1% depending on the input amount
  • Achieve high precision and high dynamic range of log5 with Nanoplate 26K or perform economical runs with 12 µl reactions and high-throughput options for “similar” expressed targets (up to about 4-fold expression change) on a Nanoplate 8.5K

Comparing dPCR and qPCR for quantifying gene expression and bacterial abundances in wasps

Methods using qPCR for assessing gene expression and bacterial counts in arthropods already exist. Although qPCR is a beneficial method for gene expression analysis, the method suffers from limitations including the need for reference materials. In this application note, compare the performance. In this application note, compare the performance of qPCR and dPCR in the quantification of gene expression and Wolbachia abundances in Nasonia parasitoid wasps.

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gene expression analysis

Related publications

Beletskiy A, et al. Downregulation of ribosomal protein genes is revealed in a model of rat hippocampal neuronal culture activation with GABA(A)R/GlyRa2 antagonist picrotoxin. Cells. 2024;13(5):383.

Pereira-García C et al. Unmasking the physiology of mercury detoxifying bacteria from polluted sediments. J Hazard Mater. 2024;467:133685.

Dawson MA, Cheung SN, La Frano MR, Nagpal R, Berryman CE. Early time-restricted eating improves markers of cardiometabolic health but has no impact on intestinal nutrient absorption in healthy adults. Cell Rep Med. 2024;5(1):101363.

Jurek B et al. Oxytocin accelerates tight junction formation and impairs cellular migration in 3D spheroids: evidence from Gapmer-induced exon skipping. Front. Cell. Neurosci. 2022:16.

Feliciello I et al. Regulation of ssb Gene Expression in Escherichia coli. International Journal of Molecular Sciences. 2022;23(18):10917.

Kirchhof L et al. G3BP1-UPF1-Associated Long Non-Coding RNA CALA Regulates RNA Turnover in the Cytoplasm. Non-Coding RNA. 2022;8(4):49.

Further resources

Albers J, et al. A workflow combining high-accuracy cell sorting with multiplex digital PCR for single-cell gene expression analysis. QIAGEN and cellenONE, 2024.