The QIAquick and MinElute systems use a simple bind-wash-elute procedure with spin columns or a vacuum manifold.
Analysis of PCR products before (b) and after (a) purification with the QIAquick PCR Purification Kit is shown. Samples were analyzed on a 1% TAE agarose gel. M: markers.
GelPilot Loading Dye contains three tracking dyes to facilitate optimization of DNA resolution.
pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case, the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.
