RT2 Profiler PCR Array Modification
For gene expression profiling using a partially modified pathway or disease panel
The RT2 Profiler PCR Array Modification combines cataloged RT2 Profiler PCR Arrays with up to 4 mRNAs unique to your research, while still retaining the patented controls for reverse transcription efficiency, qPCR performance and genomic DNA contamination. Add your important 4 mRNAs to a cataloged array and make a pathway unique to your research! Buy Products
The RT2 Profiler PCR Array Modification is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
SensitivityThe sensitivity of the RT2 First Strand Kit enables gene expression analysis using as little as 1 ng of total RNA. With preamplification, more of your targets can be accurately quantitated using the complete PCR array system. ReproducibilityThe complete PCR array system demonstrates strong correlations across technical replicates, lots and instruments with average correlation coefficients >0.99, ensuring reliable detection of differences in expression between biological samples. SpecificityThe PCR array system, with high-quality input RNA, yields single bands of the predicted size without primer-dimers or other secondary products, therefore providing highly accurate real-time PCR results. Uniform PCR amplification efficiencyUniform PCR amplification efficiency is required for the PCR array technology to allow accurate comparisons of gene expression across all genes and all samples. The unique combination of our proprietary primer design algorithm and rigorous testing of every primer assay guarantees the high performance of every primer assay on PCR arrays. A guaranteed systemRT2 Profiler PCR Arrays are tested and optimized in combination with the RT2 SYBR® Green qPCR Mastermixes and the RT2 First Strand Kit. This testing means that RT2 Profiler PCR Array performance is guaranteed when all three of these components are used together.
Principle
RT2 Profiler PCR Arrays are reliable tools for analyzing the expression of a focused panel of genes. Each 96-well plate, 384-well plate or 100-well disc PCR array includes SYBR Green-optimized primer assays for a thoroughly researched panel of relevant, pathway- or disease-focused genes. RT2 Profiler PCR Arrays can also be customized to contain a panel of genes tailored to your specific research interests. The high-quality primer design and RT2 SYBR Green qPCR Mastermix formulation enable the PCR array to amplify 96 or 384 different gene-specific products simultaneously under uniform cycling conditions.
This combination provides the RT2 Profiler PCR Array with the specificity and the high amplification efficiencies required for accurate real-time SYBR Green results. PCR arrays are easy to use in any research laboratory. RT2 Profiler PCR Arrays are sensitive enough for use with RNA prepared from regular samples (0.1–5 µg RNA), FFPE samples and small samples (1–100 ng RNA).
Procedure
Simply mix the cDNA template with the appropriate ready-to-use PCR mastermix, aliquot equal volumes to each well of the same plate and run the real-time PCR cycling program (see flowchart "Simple procedure"). RT2 Profiler PCR Arrays are compatible with all QIAGEN, ABI, Bio-Rad, Eppendorf, Roche and Stratagene instruments. Flexible layout and controls RT2 Profiler PCR Arrays are available in 96-well plate, 384-well plate and 100-well disc formats, and are used to monitor the expression of 84 or 370 genes related to a disease state or pathway, plus 5 housekeeping genes (see "Modified PCR array 96-well plate layout"). Each RT2 Profiler PCR Array also includes control elements for:
Easy-to-use data analysis Data can be analyzed using an easy-to-use Excel-based data analysis template or Web-based software. Data analysis is based on the ΔΔCT method with normalization of the raw data to either housekeeping genes.
Applications
RT2 PCR Profiler Arrays can be used in all areas of biological and medical research, including:
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