The QIAseq Single Cell DNA Library Kit UDI provides a complete solution for whole genome sequencing from isolated single cells or low amounts of genomic DNA. The kit includes all reagents required for cell lysis, whole genome amplification using REPLI-g DNA polymerase, enzymatic DNA fragmentation and PCR-free NGS library preparation compatible with Illumina NovaSeq and other NGS instruments. This kit provides comprehensive genome coverage and exceptional sequence fidelity, reducing false positives and minimizing dropouts. Due to the PCR-free workflow, this kit is highly suited for the analysis of aneuploidy, copy number and sequence variation in single cells from rare samples. The kit contains all reagents necessary for DNA library construction, including QIAseq Unique Dual-Index Y-Adapters and QIAseq Beads for reaction cleanup. The QIAseq Single Cell DNA Library Kit UDI can support up to 384 samples in a single flow-cell lane, increasing sample throughput and lowering sequencing costs.
The QIAseq Single Cell DNA Library Kit UDI includes protocols for unbiased whole genome amplification and for target enrichment of small genomes, such as viral DNA or mitochondrial genomes.