How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution?

Ni-NTA Agarose may be boiled in SDS-PAGE sample buffer to release any protein that remains on the matrix following elution. All proteins, regardless of whether they bind to Ni-NTA or to the agarose-moiety, will be recovered by this procedure.
icon_0011_idea-s

Can’t find what you are looking for?

Browse the FAQ base with our FAQ search.