| Cell Line Species/Tissue: |
Human
/
Kidney Epithelial |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10 |
Reporter System:
|
|
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
|
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
|
Amount of Enhancer (µl):
|
16 |
Amount of Reagent (µl):
|
25 |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| cDNAs cloned into the CMV-driven pcDNA3.1/GS vector. Alternative to protein microarrays for the identification of drug targets and an expression cloning system for the discovery of gene products that alter cellular physiology |
| References: |
| Microarrays of cells expressing defined cDNAs, Junaid Ziauddin & David M. Sabatini, Nature/Vol 411/3 May 2001 |