QIAamp DNA Stool Mini Kit

For isolation of DNA from stool
  • Rapid isolation of high-quality, ready-to-use DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors

The QIAamp DNA Stool Mini Kit provides silica membrane-based purification of up to 30 μg genomic, bacterial, viral, and parasite DNA from fresh or frozen human stool or other sample types with high concentrations of PCR inhibitors. The combined action of InhibitEX, a unique adsorption resin, and an optimized buffer leads to removal of PCR inhibitors. The convenient QIAamp spin-column procedure provides purification in only 50 minutes. Purification of DNA using the QIAamp DNA Stool Mini Kit can be automated on the QIAcube.

The QIAamp® DNA Stool Mini Kit (50) (cat. no. 51504) and the InhibitEX Tablets (100) (cat. no. 19590) are no longer manufactured. Instead, we offer you the QIAamp Fast DNA Stool Mini Kit (50) (cat. no. 51604) as an alternative product.

产品 货号 目录价:
QIAamp DNA Stool Mini Kit (50)
For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEX tablets, Buffers, Collection Tubes (2 ml)
51504
询价

QIAamp DNA Stool Mini Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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Performance

The QIAamp DNA Stool Mini Kit allows rapid purification of DNA from fresh or frozen human stool or other sample types with high concentrations of PCR inhibitors. Up to 220 mg stool can be processed routinely, and larger amounts can be processed with additional Buffer ASL. Typical yields of 10–30 µg are obtained in 50 minutes, and DNA is eluted in 200 µl.  The purified DNA is sized up to 50 kb. DNA of this length denatures completely and has the highest amplification efficiency. Highly pure DNA is ready for direct use in downstream amplification reactions (see figure "Removal of PCR inhibitors").

DNA that has been purified using the QIAamp DNA Stool Mini Kit can be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, infectious disease research, and screening.

Principle

DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. No phenol–chloroform extraction is required. PCR inhibitors are removed by the combined action of InhibitEX, a unique adsorption resin, and an optimized buffer. Rigorous lysis using proteinase K ensures high yields of all types of DNA common in stool, including colorectal epithelial cells, bacteria, viruses and other gastrointestinal pathogens.

Highly pure DNA ready for direct use in downstream amplification reactions is purified in about 50 minutes. QIAamp sample preparation technology is fully licensed.

Procedure
The QIAamp DNA Stool Mini Kit simplifies isolation of DNA from stool with a fast spin-column procedure (see flowchart "Procedure"). Optimized buffers and enzymes lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp spin column. PCR inhibitors are removed by the combined action of InhibitEX and an optimized buffer. Proteinase K lysis ensures high yields of all types of DNA common in stool. Remaining impurities are efficiently removed in two wash steps. Amplification-ready DNA is then eluted in low-salt buffer.
Applications

The QIAamp DNA Stool Mini Kit is for isolation of genomic, bacterial, viral, and parasite DNA from the following sample types:

Fresh stool
Frozen stool
Other sample types with high concentrations of PCR inhibitors
特点
参数
应用 PCR, blotting
洗脱体积 200 µl
规格 Spin column
主要样本类型 Stool samples
处理 Manual (centrifugation or vacuum)
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 Genomic DNA, bacterial DNA, parasite DNA, viral DNA
样本量 220 mg
技术 Silica technology
每次运行或制备时间 50 minutes
产量 10–30 µg

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试剂盒操作手册
2
For DNA purification from stool samples. Version June 2012
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For DNA purification from stool samples
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用户开发的实验方案
2
The protocol describes the preservation and concentration of stool samples (in preparation for microscopic examination for intestinal parasites), using two systems from Meridian Biosciences, followed by isolation of DNA from the stool samples for pathogen detection using the QIAamp DNA Stool Mini Kit.
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As starting material, 5 g soil was mixed with different amounts of Bacillus subtilis cells. Sensitivity was 5 x 103 cells/5g soil.
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安全数据表
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Download Safety Data Sheets for QIAGEN product components.
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