QIAamp DNA Micro Kit

For purification of genomic and mitochondrial DNA from small samples
  • Rapid purification of high-quality DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors

The QIAamp DNA Micro Kit simplifies purification of genomic and mitochondrial DNA with a fast spin-column procedure. Optimized protocols are included for use with small amounts of fresh or frozen blood, tissue, and dried blood spots. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 μl. Purification of DNA using the QIAamp DNA Micro Kit can be automated on the QIAcube.

製品名 Cat. no. List price:
QIAamp DNA Micro Kit (50)
For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)
56304
¥35,000

QIAamp DNA Micro Kit  は分子生物学実験用です。疾病の診断、治療または予防の目的には使用することはできません。


  • Main Image Navi
パフォーマンス
The resulting purified DNA is free of proteins, nucleases, and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR (see figure "Efficient purification of DNA from small sample sizes") and laser microdissection (LMD) PCR (see figure "Laser microdissection PCR"). DNA purified with the QIAamp DNA Micro Kit may also be used in short-tandem repeat (STR) genotyping, single-nucleotide pylomorphism (SNP) genotyping, or pharmacogenomic research.
原理

The QIAamp DNA Micro Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 µl. DNA binds specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. No phenol–chloroform extraction is required. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp DNA Micro technology yields genomic and mitochondrial DNA from small samples ready to use in PCR and blotting procedures.

QIAamp sample preparation technology is fully licensed, allowing QIAamp purified nucleic acids to be used in any molecular assay or other downstream application without risk of patent infringement.

操作手順
The QIAamp DNA Micro Kit simplifies isolation of DNA from small samples with a fast spin-column procedure (see flowchart "Procedure"). The QIAamp DNA Micro Kit uses well-established technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes (e.g., 1–100 µl whole blood, blood cards of ~3 mm diameter, urine, or <10 mg tissue or laser microdissected tissue). Fresh or frozen blood, tissue, and dried blood spots can be purified in 30 minutes and eluted in 20–100 µl.
アプリケーション

The QIAamp DNA Micro Kit procedure is suitable for a wide range of sample materials, including:

  • Small volumes of blood
  • Blood cards
  • Urine
  • Small tissue samples, including laster microdissections
Feature
Specifications
Applications Real-time PCR, STR analysis, LMD-PCR
Elution volume 20–100 µl
Format Spin column
Main sample type Whole blood
Processing Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA, mitochondrial DNA
Sample amount 1–100 µl
Technology Silica technology
Time per run or per prep 30 minutes
Yield <3 µg

You are not authorized to download the resource

FAQ
14
FAQ ID -1188
表示
FAQ ID -1547
表示
FAQ ID -473
表示
FAQ ID -618
表示
FAQ ID -754
表示
FAQ ID -761
表示
FAQ ID -908
表示
FAQ ID -926
表示
FAQ ID - 3560
表示
FAQ ID -728
表示
FAQ ID -909
表示
FAQ ID -730
表示
FAQ ID -12
表示
FAQ ID -305
表示
キットハンドブック
2
少量の血液、乾燥血液班、尿、組織、LMD採取した組織からのゲノムDNAの精製 ゲノムDNAのクリーンアップ
詳細を表示
For purification of genomic DNA from small volumes of blood, dried blood spots, swabs, forensic case work samples, chewing gum, urine, tissues, laser-microdissected tissues; For cleanup of genomic DNA
詳細を表示
MSDS
1
Download Safety Data Sheets for QIAGEN product components.
表示
安全データシート
2
画像