Heatmap comparing miRNA profiles from cancers of the thyroid, ovary, colon, pancreas, kidney and breast. There are distinct differences in the miRNA expression in the various types of cancers. This information can be used to identify classifiers for different types of cancers or as biomarkers to monitor the effects of therapy on cancer prognosis and progression.
(A) Plasma was stored at room temperature for the time indicated before PCR analysis. All four miRNAs were shown to be stable, even when left at room temperature for 48 hours. (B) Plasma samples were subjected to multiple freeze-thaw cycles. miRNA expression was stable even after 6 freeze-thaw cycles.
Each of the four cancer types (pancreatic, lung, colon and liver) has a unique miRNA expression signature.
Most of the RNA is found in the buffy coat. It is important not to contaminate the serum/plasma with RNA from this fraction.
The miRCURY LNA miRNA Serum/Plasma Focus PCR Panels are designed for profiling 175 human miRNAs commonly found in serum and plasma. Real-time PCR was performed using triplicate RT reactions on total RNA purified from >500 of serum. Average expression levels in Cq (quantification cycle) values for miRNAs profiled with the Serum/Plasma Focus miRNA panel are shown. hsa-miR-16, which is known to be highly expressed in serum/plasma, and miR-451 are considered potential indicators of hemolysis and blood cell contamination in serum/plasma (shown in orange). Other interesting plasma miRNAs are highlighted in red.
Cq values of miRNAs isolated from 5 ml normal urine supernatant or 1 ml cerebrospinal fluid using the miRCURY Exosome Cell/Urine/CSF Kit, followed by RNA extraction. Samples were profiled using the miRCURY LNA miRNA Urine Exosome Focus PCR Panel or the miRCURY LNA miRNA CSF Exosome Focus PCR Panel, respectively.