Products
The SARS-CoV-2 N1+N2 Assay Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
- Primers based on the CDC primer design targeting N1 and N2
- Probes coupled with FAM as a reporter dye for co-detection of both targets in the same channel
- Primer and probe mixture purified by HPLC at a 20x concentration
Product Details
The SARS-CoV-2 N1+N2 Assay Kit is compatible with the QIAprep& Viral RNA UM Kit.
Principle
The SARS-CoV-2 N1+N2 Assay is a mixture of four primers and two probes purified by HPLC at a 20x concentration. Therefore, only 1 µl of the assay is needed per 20 µl reaction.
The four primers are based on the CDC design, targeting the regions N1 and N2 of the viral genome. The two probes are coupled with FAM as a reporter dye and use ZEN™ quenchers for enhanced sensitivity (see table).
The SARS-CoV-2 N1+N2 Assay Kit does not include positive control.
Please ensure to follow the reaction setup and cycling condition provided by the suppliers of your RT-PCR chemistries and PCR cyclers to achieve the best performance.
| Primer | Concentration in 20x [µM] | Concentration per reaction [µM] | Sequence (5’-3’) |
|---|---|---|---|
| 2019-nCoV_N1-F | 16 | 0.8 | GAC CCC AAA ATC AGC GAA AT |
| 2019-nCoV_N1-R | 16 | 0.8 | TCT GGT TAC TGC CAG TTG AAT CTG |
| 2019-nCoV_N2-F | 16 | 0.8 | TTA CAA ACA TTG GCC GCA AA |
| 2019-nCoV_N2-R | 16 | 0.8 | GCG CGA CAT TCC GAA GAA |
| 2019-nCoV_N1-P | 5 | 0.25 | FAM-ACC CCG CAT /ZEN/ TAC GTT TGG TGG ACC-3IABkFQ |
| 2019-nCoV_N2-P | 5 | 0.25 | FAM-ACA ATT TGC /ZEN/ CCC CAG CGC TTC AG-3IABkFQ |
Applications
The SARS-CoV-2 N1+N2 Assay Kit is intended for molecular biology applications such as epidemiological research using real-time RT-PCR.
Resources
Brochures & Guides (1)
Quick-Start Protocols (1)
Safety Data Sheets (2)
Certificates of Analysis (1)