QIAsymphony DNA Kits
For automated purification of DNA from 1–96 samples
QIAsymphony DNA Kits enable automated purification of DNA from a broad range of sample types on the QIAsymphony SP with sample volumes of up to 1 ml. Genomic DNA can be purified from human whole blood, buffy coat, human and animal tissues, formalin-fixed paraffin-embedded tissue (FFPE), cultured cells, and bacterial cultures. The QIAsymphony DNA Mini Kit also enables purification of viral nucleic acids from human whole blood. Optimized protocols provide high yields of pure DNA, and flexible elution volumes allow DNA concentration to be easily optimized for each downstream application. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.
QIAsymphony DNA Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Automated purification of DNA on the QIAsymphony SP provides high yields of DNA (see figure "High yields of DNA from human whole blood"). Spectrophotometric analysis showed that the DNA was highly pure (see figure "Highly pure DNA"). DNA gave high-performance results in downstream PCR of a single-copy gene with no inhibition of PCR observed with 1 µl or 10 µl eluate in the amplification reaction (see figures "High-performance results in PCR — A" and "High-performance results in PCR — B").
QIAsymphony DNA Kits combine the speed and efficiency of silica-based purification of genomic DNA with the convenient handling of magnetic particles (see figure "Efficient processing of magnetic particles"). Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure, including accessory enzymes and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure "Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.
For tissues, cultured cells, and bacteria protocols, manual sample pretreatment is required. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute. The user can choose between different elution volumes depending on the protocol. The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples are processed in a single run.
DNA purified using QIAsymphony DNA Kits is of high quality and is ready for use in downstream applications, such as:
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