REPLI-g FFPE — a Novel Method for Reliable Amplification of Genomic DNA Derived from Fixed Tissue Samples

Isolation of sufficient genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue is often difficult due to the low amount of DNA available. In addition, only DNA from specific regions of the tissue sample might be of interest for specific genetic analysis in cancer research. A reliable method for the amplification of small amounts of genomic DNA derived from FFPE tissue samples is required. Multiple Displacement Amplification (MDA) is the most reliable method for amplifying genomic DNA. The unique DNA polymerase used in MDA exhibits proofreading activity and a much higher processivity compared to the Taq DNA polymerases used for PCR-based whole genome amplification (WGA) methods. This minimizes locus and allele bias derived from the DNA amplification process. Unfortunately, standard MDA techniques fail to work with small DNA fragments typically derived from FFPE samples. Here, we describe a novel MDA method that enables a successful amplification of genomic DNA derived from FFPE samples, providing high yields of DNA for multiple genetic testing and analysis.

Dr. Gerald Schock, Associate Director PyroMark, QIAGEN GmbH

Gerald Schock

Dr. Gerald Schock is Associate Director for QIAGEN’s Pyrosequencing product line. Dr. Schock studied Biology and Biochemistry at the University of Mainz, Germany and at the University College of Wales, Aberystwyth, UK. He joined QIAGEN after receiving his Ph.D. in 1996, and has held positions in Sales, Regional Marketing, and Global Marketing, where he built up the epigenetics portfolio before assuming responsibility for the Life Science Pyrosequencing portfolio.