DNA was purified from 7 stool samples using the QIAamp Fast DNA Stool Mini Kit and solutions from 2 alternative suppliers. Yields were measured by UV spectrophotometry. Yields were invariably highest with the QIAamp Fast DNA Stool Mini Kit.
DNA was isolated from 6 stool samples using either the QIAamp Fast DNA Stool Mini Kit or another standard column method. CT values were measured with an inhibitor-sensitive PCR system. In most samples using the standard purification method the target could not be amplified due to PCR inhibition. The QIAamp Fast DNA Stool Mini Kit efficiently removed inhibitors and enabled amplification with high sensitivity.
The novel InhibitEX Buffer included in the QIAamp Fast DNA Stool Mini Kit reduces handling time of samples and streamlines the purification to as little as 25 minutes.
DNA from a stool sample was purified using the QIAamp Fast DNA Stool Mini Kit and solutions from 2 alternative suppliers. Equal volumes of resulting eluates with isolated DNA were used to amplify the human Alu element. Represented in the amplification plot are the means of 2 replicates. The QIAamp Fast DNA Stool Mini Kit produced the lowest CT values.
