Product FAQ - QIAGEN



Product FAQ Print Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance Sorted by relevance Can the miScript Single Cell qPCR Kit be used to quantify piRNA expression? FAQ ID - 3567 View Can the miScript Single Cell qPCR Kit be used with plant cells? FAQ ID - 3568 View Are any of the miScript Single Cell qPCR Kit components interchangeable with miScript II RT Kit, miScript Plant RT Kit, miScript PreAMP PCR Kit, or miScript Microfluidics PreAMP Kit components? FAQ ID - 3569 View Should miScript PreAMP Primer Mixes be used in conjunction with the miScript Single Cell qPCR Kit? FAQ ID - 3570 View Is the cDNA cleanup step in the miScript Single Cell qPCR Kit workflow absolutely required? FAQ ID - 3571 View I have accidentally pipetted beads when removing the eluate after cDNA cleanup. What should I do? FAQ ID - 3572 View Does the miScript Single Cell qPCR Kit contain controls? FAQ ID - 3574 View When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells? FAQ ID - 3576 View I do not see any miRNA expression for my miRNAs of interest after performing single cell miRNA expression analysis with the miScript Single Cell qPCR Kit and miScript miRNA PCR Arrays or miScript Primer Assays. What could be the cause? FAQ ID - 3577 View What is the amplicon size of PCR products generated with the miScript Single Cell qPCR System? FAQ ID - 3578 View What is the dissociation curve temperature of PCR products generated with the miScript Single Cell qPCR System? FAQ ID - 3579 View When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells? FAQ ID - 3575 View How should data be analyzed when the miScript Single Cell qPCR Kit is used with miScript miRNA PCR Arrays or miScript Primer Assays? FAQ ID - 3581 View Do you recommend normalizing single cell miRNA expression data to a “housekeeping” gene or miRNA? FAQ ID - 3580 View What single cell isolation methods are recommended for use with the miScript Single Cell qPCR Kit? FAQ ID - 3582 View How would I provide information on the QuantiTect Primer assays in publications when the sequence is confidential? FAQ ID - 3593 View In which tubes is clot detection possible on QIAsymphony? FAQ ID - 3589 View Which barcodes on sample tubes are accepted by the QIAsymphony SP? FAQ ID - 3598 View Is it possible to measure the proteolytic activity of proteins on the surface of exosomes that were isolated with ExoEasy? FAQ ID - 3599 View How can I create a report/logfile on my QIAsymphony SP system? FAQ ID - 3594 View Can I change the language on my QIAsymphony SP system? FAQ ID - 3595 View Can the Rotor-Gene Q be connected to LIMS? FAQ ID - 3596 View Can the REPLI-g Mitochondrial DNA Kit be used for species other than humans? FAQ ID - 3584 View Can I use blood, cells or bacteria with the QIAamp Fast DNA Tissue Kit? FAQ ID - 3587 View What is MOI? FAQ ID -2768 View What file format and layout do I need to upload my data into the PCR Array Data Analysis software? FAQ ID -2700 View What does the RT² qPCR Primer Assay product information mean when it says that it recognizes another transcript of the same gene? FAQ ID -2712 View What do I need in order to set up an RNAi experiment? FAQ ID -2757 View What does a typical EpiTect Methyl qPCR Assay or Array include? FAQ ID -2740 View Why did the real-time PCR yield Ct values < 12? FAQ ID -2727 View Why can't I find the DNA methylation qPCR Assay for my gene of interest? FAQ ID -2743 View Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays? FAQ ID -2713 View What would happen if I used home-made PCR master mixes or master mixes from other manufacturers with the RT² products? FAQ ID -2715 View Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries? FAQ ID -2710 View Which qPCR instrument should I use with your RT² qPCR Primer Assays? FAQ ID -2714 View Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays? FAQ ID -2706 View Will pipetting error affect the EpiTect Methyl qPCR Array results? FAQ ID -2741 View Will pipetting error affect the miRNA qPCR Assay results? FAQ ID -2728 View Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be? FAQ ID -2717 View What RT² qPCR Primer Assays are available? FAQ ID -2708 View What MOI should I use for my cells? FAQ ID -2770 View What is the most reliable transfection reagent for delivering shRNA plasmids and siRNA to cells in culture? FAQ ID -2777 View What transfection reagents are compatible with the Cignal Reporter Assays? FAQ ID -2764 View What is included in the SureSilencing shRNA Plasmids shipment? FAQ ID -2785 View What are the structures of the siRNA molecules used in RNAi studies? FAQ ID -2760 View What are the critical factors in designing the siRNA molecules to be used for RNAi studies? FAQ ID -2759 View What are the key parameters contributing to unwanted off-target effects in an RNAi experiment? FAQ ID -2775 View Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file? FAQ ID -2734 View Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment? FAQ ID -2774 View Can miScript miRNA qPCR Assays be used for pre-miRNA detection? FAQ ID -2724 View Can miScript miRNA qPCR Assays detect single nucleotide difference between miRNAs? FAQ ID -2723 View Do I need an internal methylated DNA reference in the EpiTect Methyl qPCR Array? FAQ ID -2736 View Do I need the sequence specific miRNA primers in the RT step of the miRNA qPCR Assays? FAQ ID -2725 View Do you always run samples in triplicates? FAQ ID -2703 View Can you use stained (H & E) sections with RT² PreAMP cDNA Synthesis Kit? FAQ ID -2722 View Does QIAGEN guarantee the performance of the SureSilencing shRNA Plasmids? FAQ ID -2787 View Does this method with the EpiTect Methyl qPCR Array detect methylation at specific CpG dinucleotides? FAQ ID -2737 View Can I make stable cell lines with the Cignal Reporter Assays? FAQ ID -2763 View How are the primers in the EpiTect Methyl qPCR Array designed? FAQ ID -2735 View Is it acceptable to correct for transfection efficiency, when determining the level of gene expression knock down by an shRNA expression vector? FAQ ID -2783 View On which instrumentation will the RT² Profiler PCR Array work? FAQ ID -2719 View What are the advantages of using the SureSilencing shRNA Plasmids over siRNA? FAQ ID -2786 View Should I use monoclonal or polyclonal antibodies in EpiTect ChIP assays? FAQ ID -2747 View The EpiTect ChIP qPCR primers I used show very high Ct. Are there any solutions? FAQ ID -2750 View The pathway reporter luciferase activity values are greater than the positive control, is there a problem? FAQ ID -2766 View The pathway reporter luciferase activity values are less than the negative control, is there a problem? FAQ ID -2767 View How can I prevent the evaporation of reaction volume from the wells EpiTect Methyl qPCR Arrays? FAQ ID -2742 View How can RNA interference be used as a life science research tool? FAQ ID -2756 View How can I be sure that the restriction enzyme digestion is complete in the EpiTect Methyl qPCR system? FAQ ID -2731 View How can I normalize my EpiTect ChIP results? FAQ ID -2751 View What is the recommended amount of input template for each RT² qPCR Primer Assay? FAQ ID -2716 View What is the RT² Profiler PCR Array? FAQ ID -2718 View Can the EpiTect Methyl qPCR System technology reliably characterize heterogenous tissue samples? FAQ ID -2732 View Can users insert custom transcriptional regulatory elements or promoters into the Cignal Reporter Assays? FAQ ID -2765 View Can you custom design the Epitect qPCR primers for the CpG island outside of the defined promoter region? FAQ ID -2744 View Can I use your EpiTect One-Day kit with transcription factors? FAQ ID -2752 View Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased? FAQ ID -2709 View Can I manually set the threshold line? FAQ ID -2702 View Are EpiTect Methyl qPCR Arrays or Assays applicable to FFPE samples? FAQ ID -2733 View Are there any unique elements that need to be incorporated into an shRNA expression vector? FAQ ID -2772 View Can I use the default PCR program to run EpiTect Methyl qPCR Arrays or Assays? FAQ ID -2745 View Can I use your EpiTect ChIP system in a RNA study? FAQ ID -2748 View Can I use total RNA for the miRNA PCR Arrays or Assays? FAQ ID -2726 View Can I reliably detect intermediately methylated DNA with EpiTect Methyl qPCR Array technology? FAQ ID -2738 View Can I transform the Cignal Reporter Assays? FAQ ID -2762 View Can I use fluorescence microscopy to assess shRNA plasmid transfection efficiency in my model cell line of interest, when using a vector expressing a GFP reporter gene? FAQ ID -2782 View How many experiments can I do with the Cignal Lenti Reporter Assays? FAQ ID -2769 View How many housekeeping genes are included in each PCR Array? FAQ ID -2704 View How reliable are the results from the miScript Primer Assays? FAQ ID -2730 View What are the advantages of EpiTect ChIP qPCR Assays and Arrays? FAQ ID -2721 View What are EpiTect ChIP qPCR Assays? FAQ ID -2720 View How do I choose EpiTect ChIP-grade antibodies? FAQ ID -2746 View How do you determine the efficiency using the PCR array? FAQ ID -2701 View How does QIAGEN control the quality of the RT² qPCR Primer Assays? FAQ ID -2711 View How do I choose between the DNA-based Cignal Reporter Assays and the Cignal Lenti Reporter Assays? FAQ ID -2761 View Which Microbial qPCR Mastermix should I use? FAQ ID — 3395 View What are the storage conditions for the Microbial DNA qPCR products? FAQ ID — 3394 View 3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? View 3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube? View 3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit? View Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care