Cat. No. / ID: 931255
Cat. No. / ID: 931236
QIAsymphony DNA Kits enable automated purification of DNA from a broad range of sample types on the QIAsymphony SP with sample volumes of up to 1 ml. Genomic DNA can be purified from human whole blood, buffy coat, human and animal tissues, formalin-fixed paraffin-embedded tissue (FFPE), cultured cells, and bacterial cultures. The QIAsymphony DNA Mini Kit also enables purification of viral nucleic acids from human whole blood. Optimized protocols provide high yields of pure DNA, and flexible elution volumes allow DNA concentration to be easily optimized for each downstream application. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.
For tissues, cultured cells, and bacteria protocols, manual sample pretreatment is required. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute. The user can choose between different elution volumes depending on the protocol. The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples are processed in a single run.
|Protocol||Kit||Sample material||Processed volume|
|DNA Blood 200||QIAsymphony DNA Mini Kit (192)||Human whole blood||200 μl|
|DNA Blood 400||QIAsymphony DNA Midi Kit (96)||Human whole blood||400 μl|
|DNA Blood 1000||QIAsymphony DNA Midi Kit (96)||Human whole blood||1000 μl|
|DNA Buffy Coat 200||QIAsymphony DNA Mini Kit (192)||Buffy coat||200 μl|
|DNA Buffy Coat 400||QIAsymphony DNA Midi Kit (96)||Buffy coat||400 μl|
|Tissue LC 200||QIAsymphony DNA Mini Kit (192)||Pretreated sample lysates from tissue, FFPE tissue, cultured cells, and bacterial cultures; for example: heart (25 mg), lung (25 mg), kidney (25 mg), muscle (50 mg), Jurkat cells (2 x 106cells)*||200 μl|
|Tissue HC 200||QIAsymphony DNA Mini Kit (192)||Pretreated sample lysates from tissue, cultured cells, and bacterial cultures; for example: spleen (25 mg), liver (25 mg), Jurkat cells (1 x107 cells), bacteria (4 x 109 cells)*||200 μl|
|Virus Blood 200||QIAsymphony DNA Mini Kit (192)||Human whole blood||200 μl|
DNA purified using QIAsymphony DNA Kits is of high quality and is ready for use in downstream applications, such as:
Total DNA was purified from human whole blood (3 donors, 6 replicates each) on the QIAsymphony SP using the QIAsymphony DNA Mini Kit (200 µl) or QIAsymphony DNA Midi Kit (1000 µl). Blood was collected in BD Vacutainer K2E tubes and stored at 5–8°C prior to use. Yield was determined by spectrophotometric analysis.
|Applications||PCR, Real-Time PCR, Genotyping|
|Sample type||Blood; Cell culture (only Mini Kit); Tissue (only Mini Kit)|
|Elution volume||Mini Kit: 50-200 µl; Midi Kit: 100-500 µl|
|Sample amount||Mini Kit: 200 µl blood, up to 50 mg tissue or up to 1 x 10e7 cells; Midi Kit: 400 µl or 1000 µl blood|
|Yield||Mini Kit: 4-10 µg from 200 µl blood; Midi Kit: 8-20 µg from 400 µl blood or 15-50 µg from 1000 µl blood|
No. Modified QIAGEN protocols or custom protocols for QIAsymphony SP can be developed to meet your specific requirements and purchased from QIAGEN.
Yes. Any number of samples between 1 and 96 can be processed on the QIAsymphony SP.
No. We do not recommend use of carrier RNA with the QIAsymphony Virus Blood 200 Protocol.
In general, magnetic particles are not carried over into QIAsymphony eluates. However, if high amounts of genomic DNA are purified (large number of white blood cells in the sample) magnetic-particle carryover may be observed. If carryover does occur, magnetic particles in eluates will not affect most downstream applications. If magnetic particles need to be removed before performing downstream applications, tubes or plates containing eluates should first be placed in a suitable magnet and the eluates transferred to a clean tube.
Eluates obtained on the QIAsymphony SP can be stored at 2–8°C for 3 days before use in downstream applications and should be placed at –20°C or –80°C for long-term storage.
DNA yields strongly depend on the type and source of the tissue processed on the QIAsymphony SP. Approximately 70–80 µg DNA can be isolated from 25 mg of spleen, 40–50 µg from 25 mg liver, and 10–15 µg from 50 mg muscle.
The maximum amount of tissue that can be processed on the QIAsymphony SP using QIAsymphony DNA Mini Kit is 50 mg, and the maximum number of cultured cells is 1 x 107.
See details for upper limits of various starting materials in the table below:
|Sample type||Sample amount||Protocol|
|Heart||25 mg||Tissue Standard|
|Spleen||25 mg||Tissue High Content|
|Lung||25 mg||Tissue Standard|
|Liver||25 mg||Tissue High Content|
|Kidney||25 mg||Tissue Standard|
|Muscle||50 mg||Tissue Standard|
|Mouse tail||0.8 cm||Tissue Standard|
|Jurkat Cells||2 x 10e6||Tissue Standard|
|Jurkat Cells||1 x 10e7||Tissue High Content|
A total of 192 samples can be processed with one QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol (96 samples per reagent cartridge).
Sample volumes of 200 µl can be processed with the Virus Blood 200 Protocol using the QIAsymphony DNA Mini Kit.
Yes. Samples have to be completely lysed and homogenized prior to automated processing on the QIAsymphony SP platform using QIAsymphony DNA Kits.
Samples are lysed by Proteinase K digestion in lysis buffers optimized for tissue, cultured cells, and bacteria, respectively. For lysis of gram-positive bacteria, lysozyme is used in addition to Proteinase K. Lysed samples are homogenized by pipetting up and down, and insoluble material is removed by centrifugation (see the QIAsymphony DNA Handbook for details).
Samples should be processed on the QIAsymphony within 24 hours of collection. Samples should be transported and stored at 2–25°C.
Human whole blood samples treated with EDTA or citrate can be processed using the QIAsymphony Virus Blood 200 protocol with the QIAsymphony DNA Mini Kit. We do not recommend using heparin-treated blood samples.
Yes. The DNA Tissue protocols on the QIAsymphony SP purify genomic and mitochondrial DNA.
Yes. QIAsymphony DNA Investigator Kit protocols purify both genomic and mitochondrial DNA.
Final elution volumes of 60 µl, 85 µl, 110 µl, or 165 µl can be selected using the QIAsymphony Virus Blood 200 protocol. Please note that the initial elution volumes are 95 µl, 120 µl, 145 µl, or 200 µl. When calculating the amount of internal control(s) as well as the titer of the processed sample, it is necessary to take into consideration the initial volume of elution buffer that is used for each sample.
No. Protocols on the QIAsymphony SP cannot be modified by users. However, protocols allow elution volumes to be selected within a predefined range. Customized protocols for QIAsymphony SP will be available on request.
The High Content protocol for DNA extractions on the QIAsymphony SP is optimized for samples with an expected DNA yield of more than 30 µg. Up to 90 µg DNA can be purified from tissues with high cell densities (e.g., spleen) or large numbers of cultured cells.
Depending on the application, elution volumes on the QIAsymphony SP range from 30 µl to 500 µl.
Various types of tissue, cultured cells, and bacterial pellets can be processed using the tissue protocols for the QIAsymphony DNA Kits on the QIAsymphony SP.
No. The use of internal controls is not mandatory for use with the QIAsymphony Virus Blood 200 Protocol. If an internal control is not required, Buffer ATE without internal control is added to the samples. Buffer ATE must be placed in slot A of the sample drawer.
The target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony is viral DNA from released as well as cell-associated viruses, co-purified with genomic DNA from blood cells.