QIAamp DNA Blood Midi Kit
For purification of up to 60 µg genomic, mitochondrial, or viral DNA from blood and related body fluids
- Rapid purification of high-quality, ready-to-use DNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Complete removal of contaminants and inhibitors
QIAamp DNA Blood Midi Kits provide silica-membrane-based DNA purification for labs that process whole blood, plasma, serum, and other body fluids. The QIAamp DNA Blood Midi Kit is designed for processing 0.3–2 ml fresh or frozen human whole blood. The QIAamp Midi spin columns can be easily processed in a centrifuge or on vacuum manifolds (additional Buffer AW1 [Cat. no. 19081] and Buffer AW2 [Cat. no. 19072] are required for use with vacuum manifolds).
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製品名
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Cat. no.
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List price:
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QIAamp DNA Blood Midi Kit (20)
For 20 DNA midipreps: 20 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml)
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51183
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QIAamp DNA Blood Midi Kit (100)
For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml)
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51185
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QIAamp DNA Blood Midi Kit は分子生物学実験用です。疾病の診断、治療または予防の目的には使用することはできません。
パフォーマンス
QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples (see figure "Apoptotic banding in stored blood"). The purified DNA is suitable for long-range PCR amplification (see figure "Long-range PCR") and restriction fragment length polymorphism (RFLP) analysis used, for example, for paternity testing (see figure "Paternity testing by RFLP analysis").
The QIAamp DNA Blood Midi Kit yields up to 94.5% recovery DNA, depending on the starting cell densities (see table).
| 2.5 x 105 |
3.0 |
92.3 |
| 1.0 x 106 |
11.0 |
91.7 |
| 5.0 x 106 |
62.4 |
94.5 |
| 1.0 x 107 |
116.3 |
88.1 |
原理
No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acid to be eluted in either water or a buffer provided with the kit. QIAamp DNA Blood technology yields genomic, mitochondrial, or viral DNA from blood and related body fluids ready to use in PCR and blotting procedures.
操作手順
The QIAamp DNA Blood Midi Kit simplifies isolation of DNA from blood and related body fluids with a fast spin-column procedure (see flowchart "procedure"). Fresh and frozen whole blood with common anticoagulants, such as EDTA, citrate, or heparin, may be processed. The QIAamp DNA Blood Midi Kit processes sample sizes of 0.3–2 ml, with a preparation time of 55 minutes. The typical yield from 1 ml healthy whole blood is 20–60 µg, with an elution volume of 100–400 µl.
QIAamp DNA Blood Kits are supplied with a comprehensive handbook containing protocols for DNA isolation from a number of sample sources. QIAamp sample preparation technology is fully licensed.
アプリケーション
The QIAamp DNA Blood Midi Kit provides proven QIAamp technology for purification of DNA from a variatey of materials using Midi spin columns with 15 ml collection tubes. Sample sources include:
Fresh and frozen whole blood or buffy coat
Plasma or serum
Bone marrow
Lymphocytes
Body fluids
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Feature
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Specifications
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Applications
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PCR, southern blotting
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Elution volume
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100–400 µl
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Format
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Spin column
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Main sample type
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Whole blood, body fluids
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Processing
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Manual (centrifugation or vacuum)
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Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein
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Genomic DNA, mitochondrial DNA, viral DNA
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Sample amount
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0.3–2 ml
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Technology
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Silica technology
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Time per run or per prep
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55 minutes
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Yield
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20–60 µg
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FAQ ID -1011
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FAQ ID -563
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FAQ ID -635
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FAQ ID -754
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FAQ ID -761
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FAQ ID -923
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FAQ ID -728
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FAQ ID -730
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FAQ ID -12
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FAQ ID -305
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全血、血漿、血清、体液、リンパ球からのゲノムおよびウイルスDNA大量精製用
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詳細を表示
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For large-scale genomic and viral DNA purification from whole blood, plasma, serum, body fluids, lymphocytes
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詳細を表示
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As starting material, 5 g soil was mixed with different amounts of Bacillus subtilis cells. Sensitivity was 5 x 103 cells/5g soil.
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詳細を表示
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Request a Certificate of Analysis and see the quality control data for your product.
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表示
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Download Safety Data Sheets for QIAGEN product components.
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