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Whole genome amplification of DNA from blood spots dried on FTA® paper using the REPLI-g® Single Cell Kit - (EN)
Whole Genome Amplification in 96-Wells from Single Cells using the REPLI-g® Single Cell Kit - (EN)
Whole genome amplification from single cells using the REPLI-g Advanced DNA Single Cell Kit
Whole genome amplification from single cells using the REPLI-g Advanced DNA Single Cell Kit
 
Whole genome amplification from residual cells in plasma/serum samples using the REPLI-g® Single Cell Kit - (EN)
Whole genome amplification from genomic DNA using the REPLI-g® Single Cell Kit with increased sample volumes - (EN)
Whole genome amplification from genomic DNA using the REPLI-g Advanced DNA Single Cell Kit with increased sample volumes
Whole genome amplification from genomic DNA using the REPLI-g Advanced DNA Single Cell Kit with increased sample volumes
 
Whole genome amplification from flash-frozen tissue sections using the REPLI-g® Single Cell Kit - (EN)
This protocol is optimized for whole genome amplification from flash-frozen tissue samples using the REPLI-g Single Cell Kit.
Whole genome amplification from flash-frozen tissue sections using the REPLI-g Advanced DNA Single Cell Kit
Whole genome amplification from flash-frozen tissue sections using the REPLI-g Advanced DNA Single Cell Kit
 
Whole genome amplification from buccal cells using the REPLI-g® Single Cell Kit - (EN)
This protocol is optimized for whole genome amplification from buccal cells using the REPLI-g Single Cell Kit.
Whole genome amplification from buccal cells using the REPLI-g Advanced DNA Single Cell Kit
Whole genome amplification from buccal cells using the REPLI-g Advanced DNA Single Cell Kit
 
Whole genome amplification from biopsies using the REPLI-g® Single Cell Kit - (EN)
This protocol is optimized for whole genome amplification from biopsies using the REPLI-g Single Cell Kit.
Whole genome amplification from biopsies using the REPLI-g Advanced DNA Single Cell Kit
Whole genome amplification from biopsies using the REPLI-g Advanced DNA Single Cell Kit
 
Using the QIAGEN® OneStep RT-PCR Kit with a 25 µl reaction volume and shorter reaction times for viral samples - (EN)
Using the QIAGEN® OneStep RT-PCR Kit with a 25 µl reaction volume - (EN)
Using the QIAGEN® OneStep RT-PCR Kit and Q-Solution™ with a 25 µl reaction volume - (EN)
Using the QIAGEN® OneStep RT-PCR Kit and Q-Solution® with a 25 µl reaction volume and shorter reaction times for viral samples - (EN)
Using the KRAS PCR Kit on the Rotor-Gene® Q Instrument - (EN)
Using the EGFR PCR Kit on the Rotor-Gene® Q Instrument - (EN)
Use of the EZ1 Advanced XL Flip Cap Rack - (EN)
Supplementary protocol using the Flip Cap Rack and the EZ1 Advanced XL Flip Cap Card.
Type-it® HRM® PCR protocol for use with the Applied Biosystems® 7500 Fast System - (EN)
Triplex to 5-plex real-time RT-PCR analysis using the QuantiFast® Pathogen RT-PCR +IC Kit on the Rotor-Gene® Q - (EN)
Triplex to 5-plex real-time PCR analysis using the QuantiFast® Pathogen PCR +IC Kit on the Rotor-Gene® Q - (EN)
Triplex and 4plex analysis using the QuantiTect® Virus +ROX Vial Kit and a 25 µl or 20 µl reaction volume - (EN)
Triplex analysis using the QuantiTect® Virus Kit and a 25 µl reaction volume - (EN)
Transient transfection of NIH/3T3 cells in 96-Well plates using PolyFect® Transfection Reagent - (EN)
The following protocol is optimized for transient transfection of NIH/3T3 cells in 96-well plates.
Transient transfection of HeLa-S3 cells in 96-Well plates using PolyFect® Transfection Reagent - (EN)
The following protocol is optimized for transient transfection of HeLa-S3 cells in 96-well plates. Please note that for HeLa cells the optimal amounts of DNA and PolyFect® Reagent differ from the amounts given in the protocol below. Please call one of the QIAGEN Technical Service Departments or your local distributor for an optimized HeLa protocol.
Transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect® Transfection Reagent - (EN)
The following protocol is optimized for transient transfection of HeLa-S3 cells in 60 mm dishes. Parameters for transfection using other culture formats are given in Table 1. Please note that this protocol is only for HeLa-S3 cells. Optimized protocols for HeLa cells are provided in the PolyFect® Transfection Reagent Handbook.
Transient transfection of HeLa cells in 96-Well plates using PolyFect® Transfection Reagent - (EN)
The following protocol is optimized for transient transfection of HeLa cells in 96-well plates. Please note that for HeLa-S3 cells the optimal amounts of DNA and PolyFect® Reagent differ from the amounts given in the protocol below. Please call one of the QIAGEN Technical Service Departments or your local distributor for an optimized HeLa-S3 protocol.
Transient transfection of COS-7 cells in 96-Well plates using PolyFect® Transfection Reagent - (EN)
The following protocol is optimized for transient transfection of COS-7 cells in 96-well plates.
Transient transfection of CHO cells in 96-Well plates using PolyFect® Transfection Reagent - (EN)
The following protocol is optimized for transient transfection of CHO cells in 96-well plates.
Transient transfection of 293 cells in 96-Well plates using PolyFect® Transfection Reagent - (EN)
The following protocol is optimized for transient transfection of 293 cells in 96-well plates.
Transfection of adherent cells with low concentrations of siRNA using RNAiFect™ Transfection Reagent - (EN)
Some RNAi applications require the use of low amounts of siRNA. This supplementary protocol is a modified version of the RNAiFect Transfection Reagent protocol (see RNAiFect Transfection Handbook), that is optimized for transfection of adherent cells with low siRNA concentrations.
therascreen KRAS Assay Package
Software assay package for use with the therascreen KRAS RGQ PCR Kit and Rotor-Gene Q 5plex HRM or Rotor-Gene Q MDx 5plex HRM in combination with Rotor-Gene Q Software version 2.3 and 72-well rotor.
Installation of the therascreen KRAS Assay Package is described in the therascreen KRAS RGQ PCR Kit Handbook.  
Note: Previous versions of the assay package, including the version listed in the current kit handbook, are not compatible with Rotor-Gene Q Software version 2.3. If your Rotor-Gene Q 5plex HRM or Rotor-Gene Q MDx 5plex HRM has an earlier software version installed, you can upgrade to Rotor-Gene Q Software version 2.3 by downloading the software from the Rotor-Gene Q or Rotor-Gene Q MDx product webpages.
Test Assay for Fast Reaction Mix 2.0 - (EN)
Supplementary Protocols: Single Cell WGA — Purification of Amplified DNA
Purification of DNA after whole Genome amplification using the REPLI-g Single Cell Kit
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