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This protocol provides information about scaling of reagents required for purification of DNA from 1-10 ml samples of fresh or frozen whole blood using the Gentra Puregene Blood Kit.
This protocol provides information about scaling of reagents required for purification of DNA from buffy coat prepared from 0.3-15 ml samples of whole blood using the Gentra Puregene Blood Kit.
This protocol provides information about scaling of reagents required for purification of DNA from 5-60 mg mouse tail tissue using the Gentra Puregene Mouse Tail Kit.
This protocol provides information about scaling of reagents required for purification of DNA from 100 to 5 x 108 cultured cells using the Gentra Puregene Cell Kit.
This protocol provides information about scaling of reagents required for purification of DNA from 1 x 106 - 3 x 107 fixed cells using the Gentra Puregene Tissue Kit.
This protocol provides information about scaling of reagents required for purification of DNA from 0.5-700 mg solid tissue using the Gentra Puregene Tissue Kit.
This protocol provides information about scaling of reagents required for purification of DNA from 0.5-20 mg paraffin-embedded tissue using the Gentra Puregene Tissue Kit.
Endotoxin-free DNA is essential for gene therapy research and will improve transfection into sensitive eukaryotic cells.
This protocol is designed for rapid purification of DNA from 1-2 x 106, 3-5 x 106, or 6-9 x 106 cells using the Gentra Puregene Cell Kit.
Up to 1 x 109 bacteria are disrupted and homogenized by bead-milling in a guanidine-thiocyanate-containing lysis buffer. After addition of ethanol, the sample is loaded onto an RNeasy Mini spin column. Total RNA binds to the RNeasy silica-membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in RNase-free water.
This protocol is designed for purification of DNA from up to 5 x 107 yeast cells.
This protocol is designed for purification of DNA from up to 50 mg of insects, such as drosophila.
This protocol is designed for purification of DNA from a 200 μl crude lysate.
There are two protocols: follow Protocol 1 if you want to purify total RNA containing miRNA, or follow Protocol 2 if you want to purify small RNA (includes miRNA, 5S rRNA, and tRNA) and larger RNA (>200 nt) separately.
This protocol describes the steps for setting up the AutoGenFlex 3000 workstation and the steps for DNA purification from fresh or frozen human whole blood or buffy coat samples using the AutoGenFlex 3000 workstation.
This protocol is designed for purification of DNA from up to 10 ml clotted blood using the FlexiGene DNA Kit.
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