What is the composition of Buffer RLC?

The exact composition of Buffer RLC is confidential. This buffer is a proprietary component of RNeasy Plant Kits. RNeasy Plant Kits provide two different lysis buffers: Buffer RLT, containing high concentrations of guanidine isothiocyante and Buffer RLC, comprising high concentrations of guanidine hydrochloride. In most cases, Buffer RLT is the buffer of choice due to its stronger lysis capability. However, depending on the type of starting material, Buffer RLT can cause solidification of the lysate, for example, when using maize endosperm. In these cases, the guanidine hydrochloride-containing Buffer RLC should be used.
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