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PAXgene Blood ccfDNA Tubes (100)

For collection of whole blood samples and stabilization of ccfDNA

 

  • Single-tube collection, stabilization, transport and storage
  • Immediate stabilization of circulating, cell-free DNA (ccfDNA)
  • Sample transport and storage at room temperature for up to 10 days
  • Standardized preanalytical processing of samples

The PAXgene Blood ccfDNA Tube is a plastic, closed, evacuated tube for the collection, anticoagulation, transport and storage of human whole blood samples and the stabilization of ccfDNA. When the tube is used in conjunction with the QIAsymphony PAXgene Blood ccfDNA Kit, the QIAGEN QIAamp MinElute ccfDNA Kit or the QIAamp Circulating Nucleic Acid Kit, high-quality ccfDNA can be isolated for use in downstream analytical assays.

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Cat No./ID: 768115
PAXgene Blood ccfDNA Tubes (100)
HK$9,390.00
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100 blood collection tubes (10 ml). To be used in conjunction with the QIAamp MinElute ccfDNA Kit, the QIAamp Circulating Nucleic Acid Kit (50) or the QIAsymphony PAXgene Blood ccfDNA Kit (192)
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

Product Details

Performance

PAXgene Blood ccfDNA Tubes simplify the collection and processing of whole blood for subsequent purification of  circulating, cell-free DNA (ccfDNA). The tube additive stabilizes blood cells (see "PAXgene Blood ccfDNA stabilization helps prevent hemolysis allowing distinct separation of plasma" and "PAXgene Blood ccfDNA stabilization minimizes hemolysis compared to alternative solutions") and blocks apoptosis (see "PAXgene Blood ccfDNA stabilization helps prevent apoptosis and fragmentation of gDNA").  Consequently, using PAXgene Blood ccfDNA Tubes for whole blood collection, storage and transport helps prevent the release of intracellular DNA into plasma (see "PAXgene Blood ccfDNA stabilization helps prevent release of gDNA into plasma"). ccfDNA levels are stable for up to 10 days at temperatures up to 25°C, 7 days at temperatures up to 30°C, or 3 days at temperatures up to 37°C prior to processing, so blood samples can be transported and stored to process plasma later.

Plasma generated from whole blood collected into PAXgene Blood ccfDNA Tubes and processed with the QIAsymphony PAXgene Blood ccfDNA Kit, the QIAGEN QIAamp MinElute ccfDNA Kit or the QIAamp Circulating Nucleic Acid Kit, yields high-quality ccfDNA for downstream analytical assays, including but not limited to PCR-based assays and next-generation sequencing (NGS).

Principle
The PAXgene Blood ccfDNA Tube is a plastic, closed, evacuated tube for the collection, anticoagulation, transport and storage of human whole blood samples and the stabilization of ccfDNA. The tube contains an additive that anticoagulates blood and stabilizes blood cells via a non-crosslinking stabilization solution. This helps prevent the release of intracellular DNA into the plasma and maintains constant ccfDNA levels during sample storage before processing.
Procedure
The PAXgene Blood ccfDNA Tube allows collection of 10 ml whole blood into a closed, evacuated system. Using standard venipuncture technique, blood is collected into an evacuated tube that contains 1.5 ml cell stabilization additive, resulting in a ratio of 0.15 ml additive per 1 ml blood. Isolation of ccfDNA from plasma is carried out using the QIAsymphony PAXgene Blood ccfDNA Kit, the QIAamp MinElute ccfDNA Kit or the QIAamp Circulating Nucleic Acid Kit.
Applications

ccfDNA purified from plasma generated from whole blood collected into PAXgene Blood ccfDNA Tubes can be used in a wide range of downstream applications, including:

  • PCR, including digital, multiplex and quantitative real-time PCR
  • Pharmacogenomic studies
  • SNP genotyping
  • Next-generation sequencing

 

Specifications

Features
Specifications
Additive/concentration Additive 1.5 ml
Closure type/color Pearl white BD Hemogard/Blue stopper
Draw volume 10 ml
Label type Paper
Quantity 100 tubes/case
Stabilization Up to 10 days at temperatures up to 25°C, 7 days at temperatures up to 30°C, or 3 days at temperatures up to 37°C. Note: Do not store blood-filled tubes below 2°C.
Tube size 16 x 100 mm

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Scientific Posters (16)
Ullius et al., 2015

 


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Voss et al., 2017

 


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Ullius et al., 2016

 


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Ullius et al.

 


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Ullius et al. 2019

 


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Ullius et al. 2019

 


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Babayan et al. 2019

 


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Hallemariam et al. 2019

 


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Ullius et al. 2019

 


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Krenz et al. 2019

 


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Fleischhacker et al., 2016

 


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Fleischhacker et al., 2017

 


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Groelz et al., 2017

 


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Groelz et al., 2017

 


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Alsobrook et al., 2017

 


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Murray et al., 2017

 


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Application Notes (2)

IATA-conform transport of whole blood samples in PAXgene Blood ccfDNA Tubes – a simulation and functional test


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Demonstrated 3-in-1 workflow for the simultaneous extraction of circulating tumor cell RNA, ccfDNA and leukocyte-derived gDNA from a single blood sample collected and stored in a PAXgene Blood ccfDNA Tube (RUO)


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Kit Handbooks (1)
For collection of whole blood and stabilization of ccfDNA
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