miRCURY LNA miRNA Family Power Inhibitors
For analyzing regulatory roles shared by highly related, co-expressed and functionally redundant miRNAs
miRCURY LNA miRNA Family Power Inhibitors allow you to study regulatory roles shared by highly related, co-expressed and functionally redundant miRNAs. Such functions would not be revealed in analyses using inhibitors of individual family members. These antisense oligonucleotides have perfect sequence complementarity to their targets, and when introduced into cells, they sequester the target miRNA in highly stable heteroduplexes that prevent interaction with the normal cellular partners.
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Discover unknown miRNA functionmiRNA family members are often co-expressed, either from the same pri-miR transcript or from distinct but co-regulated loci. This suggests that miRNAs within a family may share a common regulatory function. Given their identical seed sequence, miRNA family members will have many mRNA targets in common, and they are therefore likely to be functionally redundant. Therefore, analysis of miRNA family function requires simultaneous inhibition of all or several family members.
The miRCURY LNA miRNA Family Power Inhibitors allow you to observe robust phenotypes that would be either absent or weakly presented if using inhibitors of individual miRNA family members (see figure The miR-30 family positively regulates hMADS cell adipocyte differentiation). This enables you to discover new, unknown miRNA functions that you may have deduced through careful analysis of miRNA target predictions, but have not been able to prove experimentally.
DesignmiRCURY LNA miRNA Family Power Inhibitors are specifically designed to silence entire families of miRNAs, rather than individual family members. Through careful design, we have developed special family inhibitors that knock down all family members, while retaining specificity for the targeted family (see figure Example of miRNA family inhibitor design). This is achieved through the following design criteria:
CoverageWe have developed inhibitors for more than 40 human miRNA families that are conserved in mouse. miRCURY LNA miRNA Family Power Inhibitors have been designed only for miRNA families in which the design criteria described above provide an advantage over simply using a mixture of individual inhibitors of all miRNA family members.
We define miRNA families as highly related miRNAs that share an identical seed sequence (nucleotides 2–8). This is particularly important for target recognition, although the miR-200 family is one of the few exceptions to this rule.
Some very large, miRBase-defined miRNA families, such as the hsa-miR-506, hsa-miR-515 and hsa-miR-548 families, comprise miRNAs with similar sequences but with different seed sequences. These families are not represented in our miRCURY LNA miRNA Family Power Inhibitors.
Power Inhibitors are not recommended for use with cells or cell lines derived from muscle or the central nervous system (CNS). These cell types are known to be particularly sensitive to sequence-dependent toxicity of phosphorothioate-modified oligonucleotides.
miRCURY LNA miRNA Family Power Inhibitors are antisense oligonucleotides with perfect sequence complementarity to their targets. When introduced into cells, they sequester the target miRNA in highly stable heteroduplexes, effectively preventing the miRNA from hybridizing with its normal cellular interaction partners. The sequences of the oligonucleotides and their LNA spiking patterns have been carefully designed to achieve uniform high potency, regardless of the GC content of the target. The Tm is normalized to an optimal temperature, and the level of self-complementarity is kept to a minimum.
Following resuspension, miCURY LNA miRNA Family Power Inhibitors are introduced into cells with a transfection reagent or via electroporation. Phenotypic effects are at an appropriate time thereafter.
miRCURY LNA miRNA Inhibitors are primarily used miRNA functional studies by assessing the biological consequences of inhibiting miRNA activity. These effects can be assessed in a variety of ways, including using cellular assays to monitor cell proliferation, cell differentiation or apoptosis. The effects on gene expression can also be measured at the mRNA or protein level of putative miRNA targets.
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