ipsogen BCR-ABL1 mbcr Kit CE

For quantitative detection of BCR-ABL mbcr e1a2 transcripts using real-time PCR

Features

Highly accurate and sensitive quantitative assay
EAC standardized assay for reproducible results
Compliance with EU IVD Directive 98/79/EC
Easy workflow with ready-to-use solutions

Product Details

The ipsogen BCR-ABL1 mbcr Kit is an in vitro molecular diagnostic kit for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kit provides reagents optimized for reliable and sensitive detection and quantification of BCR-ABL mbcr p190 e1a2 transcripts in bone marrow or peripheral blood samples of Ph-positive acute lymphoblastic leukemia (ALL) patients previously diagnosed with a BCR-ABL mbcr fusion gene event. The test is intended to monitor efficacy of treatment in patients undergoing therapy and for minimal residual disease (MRD) follow-up to monitor disease relapse.

Performance

To ensure highest sensitivity, the ipsogen BCR-ABL1 mbcr Kit has been optimized to detect BCR-ABL mbcr p190 e1a2 and ABL transcripts and uses prediluted plasmid standards and primers and probe mixes (see figures Accurate detection of BCR-ABL mbcr plasmid standards and Reliable detection of ABL plasmid standards).

See figures

Accurate detection of BCR-ABL mbcr plasmid standards.

Reliable detection of ABL plasmid standards.

Principle

The ipsogen BCR-ABL1 mbcr Kit is a ready-to-use kit for the detection of BCR-ABL mbcr p190 e1a2 fusion gene transcripts using real-time PCR. The kit is based on the amplification and detection of specific BCR-ABL p190 e1a2 transcripts, relative to ABL control gene expression, in total RNA extracted from bone marrow or peripheral blood samples of Ph-positive acute lymphoblastic leukemia (ALL) patients previously diagnosed with a BCR-ABL mbcr fusion gene event (see figures BCR-ABL mbcr fusion gene transcript and ABL control gene transcript). The kit provides high levels of specificity, sensitivity, and reproducibility. The ipsogen BCR-ABL1 mbcr Kit provides 5 standard dilutions for BCR-ABL mbcr and 3 standard dilutions for ABL. Use of the standards enables accurate quantification of transcripts. The technology used for quantification of BCR-ABL mbcr has been standardized according to Europe Against Cancer (EAC) recommendations and the ipsogen BCR-ABL1 mbcr Kit uses this validated technology to calibrate results.

See figures

BCR-ABL mbcr fusion gene transcript.

ABL control gene transcript.

Procedure

The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using the ipsogen BCR-ABL1 mbcr Kit allows detection and quantification of BCR-ABL mbcr p190 e1a2 and ABL transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.

Applications

The ipsogen BCR-ABL1 mbcr Kit enable sensitive and reliable detection and quantification of BCR-ABL mbcr p190 e1a2 transcripts, relative to ABL control gene expression, for in vitro diagnostic use.