PCR of the single-copy MECL-1 gene was performed using DNA purified from whole blood. Genomic DNA was purified from samples of ACD-, EDTA-, and heparin-preserved human whole blood using the EZ1 DNA Blood 200 μl Kit. Template DNA was serially diluted. An aliquot of purified DNA (volume as indicated) was used in each 50 μl PCR.
DNA was purified from 200 µl blood samples using the EZ1 DNA Blood 200 µl Kit (upper lanes) and from 350 µl blood samples using the EZ1 DNA Blood 350 µl Kit (lower lanes). Lanes 1–6 and 7–12 are from the first and last of 8 processing runs on the EZ1 instrument, respectively. M: 1000 bp DNA ladder (100 ng); +: positive control; -: negative control. 2 µl (1%) of each eluate was visualized on the agarose gel.