mericon Pathogen Detection Assay

For real-time PCR detection of pathogen DNA in food or animal feed

Features

  • Highly sensitive and specific detection of a broad range of pathogens
  • Single, streamlined detection protocol for all targets
  • Dedicated mericon bacterial DNA isolation kits available
  • PCR run time of 73 minutes on the QIAGEN Rotor-Gene Q
  • Full license for PCR without additional costs
mericon Shigella spp Kit (24)

Cat. No. / ID: 290103

For 24 reactions: PCR Assay Shigella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
Kit
mericon Pathogen Kit
mericon Quant Pathogen Kit
Pathogen
Shigella spp
Cronobacter spp
Y. enterocolitica
Listeria spp
E. coli O157
VTEC stx 1/2
Vibro tiple
Salmonella spp
mericon Pathogen Detection Assay are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

mericon Pathogen Detection Assays are a suite of kits for the target-specific detection of bacterial pathogen DNA in food, animal feed, and pharmaceutical products. All of the assays use the same real-time PCR-based protocol. They are part of the comprehensive QIAGEN food safety testing portfolio, which also features dedicated sample preparation kits and assays for ingredient authentication. mericon assays perform optimally on the Rotor-Gene Q, but have also been validated for block thermal cyclers.

Performance

mericon Campylobacter spp Kit

The mericon Campylobacter spp Kit is for the specific detection of several Campylobacter subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Campylobacter DNA in a reaction and it exhibits high specificity for pathogens this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Campylobacter spp Kit cross-reactivity study").

Results of mericon Campylobacter spp Kit cross-reactivity study

Pathogen Result Pathogen Result
Campylobacter jejuni + Campylobacter coli +
Campylobacter lari + Campylobacter upsaliensis +
Salmonella abony Staphylococcus aureus
Clostridium perfringens Escherichia coli
Legionella erythra Yersinia enterocolitica
Legionella pneumophila Listeria monocytogenes
Bacillus cereus Cronobacter sakazakii
Shigella flexneri    

mericon Cronobacter spp Kit

The mericon Cronobacter spp Kit is for the specific detection of Chronobacter subspecies in enrichment cultures of food and animal feed samples. Due to a recent change in taxonomy and according to regulation of the EU Commission (365/2010) the name Cronobacter spp replaces Enterobacter sakazakii. The assay can detect as few as 10 copies of Cronobacter DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Cronobacter spp Kit cross-reactivity study").

Results of mericon Cronobacter spp Kit cross-reactivity study

Pathogen Result Pathogen Result
Cronobacter sakazakii + Cronobacter dubliensis +
Cronobacter turingensis + Cronobacter malonatus +
Cronobacter muytjensii + Legionella pneumophila
Campylobacter jejuni Listeria monocytogenes
Campylobacter coli Salmonella abony
Campylobacter lari Shigella flexneri
Campylobacter upsaliensis Yersinia enterocolitica
Staphylococcus aureus Clostridium perfringens
Bacillus cereus Escherichia coli
Legionella erythra VTEC stx1/2

mericon Quant Legionella spp Kit

The mericon Quant Legionella spp Kit is for the specific detection and quantification of several Legionella species in water after concentration (e.g., by filtration). The assay can detect as few as 10 copies of Legionella DNA in a reaction and it exhibits high specificity for pathogens of that genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Quant Legionella spp Kit cross-reactivity study").

Results of mericon Quant Legionella spp Kit cross-reactivity study

Pathogen Result Pathogen Result
Legionella pneumophila + Legionella erythra +
Legionella adelaidensis + Legionella feeleii +
Legionella impletisoli + Legionella jordanis +
Legionella israeliensis + Legionella tucsonensis +
Legionella worsleinsis + Legionella beliardensis +
Legionella spiritensis + Legionella lansingensis +
Legionella brunensis + Legionella drozanskii +
Legionella geestiana + Legionella nautarum +
Legionella santicruci + Legionella shakespeare +
Salmonella abony Bacillus cereus
Clostridium perfringens Campylobacter jejuni
Escherichia coli Yersinia enterocolitica
Staphylococcus aureus Listeria monocytogenes
Shigella flexneri Cronobacter sakazakii

mericon Quant L. pneumophila Kit

The mericon Quant L. pneumophila Kit is for the specific detection and quantification of Legionella pneumophila in water after concentration (e.g., by filtration). The assay can detect as few as 10 copies of L. pneumophila DNA in a reaction and it exhibits high specificity for L. pneumophila. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Quant L. pneumophila Kit cross-reactivity study").

Results of mericon Quant L. pneumophila Kit cross-reactivity study

Pathogen Result Pathogen Result
Legionella pneumophila + Legionella erythra
Legionella adelaidensis Legionella feeleii
Legionella impletisoli Legionella jordanis
Legionella israeliensis Legionella tucsonensis
Legionella worsleinsis Legionella beliardensis
Legionella spiritensis Legionella lansingensis
Legionella brunensis Legionella drozanskii
Legionella geestiana Legionella nautarum
Legionella santicruci Legionella shakespeare
Salmonella abony Bacillus cereus
Clostridium perfringens Campylobacter jejuni
Escherichia coli Yersinia enterocolitica
Staphylococcus aureus Listeria monocytogenes
Shigella flexneri Cronobacter sakazakii

mericon Listeria spp Kit

The mericon Listeria spp Kit is for the specific detection of several Listeria subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Listeria DNA in a reaction and it exhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Listeria Kit cross-reactivity study").

Results of mericon Listeria spp Kit cross-reactivity study

Pathogen Result Pathogen Result
Listeria monocytogenes + Listeria innocua +
Listeria welshimeri + Listeria marthii +
Listeria ivanovii + Listeria grayi +
Listeria seeligeri + Campylobacter jejuni +
Listeria rocourtiae Clostridium perfringens
Escherichia coli Legionella erythra
Cronobacter sakazakii Shigella flexneri
Legionella pneumophila VTEC stx1/stx2
Staphylococcus aureus Salmonella albony
Bacillus cereus Yersinia enterocolitica

mericon L. monocytogenes Kit

The mericon L. monocytogenes Kit is for the specific detection of Listeria monocytogenes in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of L. monocytogenes DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens, even closely-related Listeria species.

mericon Salmonella spp Kit

The mericon Salmonella spp Kit is for the specific detection of several Salmonella subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Salmonella DNA in a reaction and it exhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens.

mericon Shigella spp Kit

The mericon Shigella spp Kit is for the specific detection of several Shigella subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Shigella DNA in a reaction and it exhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens.

mericon S. aureus Kit

The mericon S. aureus Kit is for the specific detection of Staphylococcus aureus in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of S. aureus DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens.

mericon VTEC stx1/2 Kit

The mericon VTEC stx1/2 Kit is for the specific detection of the strains of Escherichia coli that produce verotoxins, also called Shiga toxins, in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of verotoxin-producing E. coli DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon VTEC stx1/2 Kit cross-reactivity study").

Results of mericon VTEC stx1/2 Kit cross-reactivity study

Pathogen Result Pathogen Result
VTEC stx1 + VTEC stx2 +
Campylobacter jejuni Escherichia coli
Campylobacter coli Clostridium perfringens
Campylobacter lari Bacillus cereus
Campylobacter upsaliensis Yersinia enterocolitica
Legionella pneumophila Listeria monocytogenes
Legionella erythra Shigella flexneri
Staphylococcus aureus Cronobacter sakazakii
Salmonella abony    

mericon Y. enterocolitica Kit

The mericon Y. enterocolitica Kit is designed for the detection of Yersinia enterocolitica in enrichment cultures of food and animal feed samples. The kit specifically detects human pathogen serotypes 0:3, 0:5, 0:6, 0:8, 0:9, and 0:5:27. The assay can detect as few as 10 copies of Yersinia enterocolitica DNA in a reaction and exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens.

mericon Vibrio triple Kit

The mericon Vibrio triple Kit is for the specific detection of Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio cholerae in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio cholerae DNA in a reaction and exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Vibrio triple Kit cross-reactivity study").

Pathogen Result Pathogen Result
Vibrio vulnificus + Staphylococcus aureus
Vibrio parahaemolyticus + Bacillus cereus
Vibrio cholerae + Salmonella abony
Vibrio alginolyticus Clostridium perfringens
Vibrio furnissii Shigella flexneri
Legionella pneumophila Escherichia coli
Campylobacter jejuni Cronobacter sakazakii
Listeria monocytogenes Yersinia enterocolitica

mericon E. coli O157 Detection Kits 

The mericon E. coli Screen Plus Kit is designed for the qualitative detection of the E. coli serotype O157 and E. coli virulence genes intimin (eae) and Shiga toxin-like proteins (stx1 and stx2). The presence of eae and stx1/stx2 suggests that further investigation into the E. coli O-serotype O157, or the non-O157 O-serotypes (O26, O45, O103, O111, O121, and O145) is required in food and animal feed samples after enrichment. The assay can detect as few as 10 copies of E. coli DNA in a reaction and it exhibits high specificity for pathogens of this species. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see table "Results of mericon E. coli O157 Screen Plus Kit cross-reactivity study" for exclusivity/inclusivity data). 

The mericon E. coli STEC O-Type Kit exhibits high specificity for E. coli strain O157:H7, O26, O45, O103, O111, O121, and O145. The kit was tested against an extensive inclusivity panel of E. coli O157:H7 and non-O157:H7 serotype strains, and an exclusivity panel of other E. coli and non-E. coli strains. All tested O157:H7 and non-O157:H7 serotype strains (O26, O45, O103, O111, O121, and O145) were successfully detected and no cross-reactivity was observed with other pathogens using 2500 copies of tested DNA.

Principle

Detection of pathogens in food testing using real-time PCR is based on the amplification of a specific region of the relevant pathogen genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.

mericon PCR Assays include an internal control to control potential inhibition. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes").

Summary of possible PCR outcomes

Internal control amplified Tested DNA amplified Meaning
+ + Sample is positive for the target DNA
+ Sample is negative for the target DNA
PCR failed due to inhibition


The PCR primer set for each assay is highly specific and targets a unique and conserved region of the tested pathogen genome that has been bioinformatically and experimentally verified.

Procedure

DNA is extracted from an enrichment culture of a sample of food or animal feed using a dedicated sample preparation kit. The mericon Bacteria Kit is for the isolation of bacterial DNA from Gram-negative bacteria. The mericon Bacteria Plus Kit is for the isolation of bacterial DNA from Gram-positive bacteria.

The isolated, pure bacterial DNA is assayed using a streamlined real-time PCR protocol that returns results in 73 minutes on QIAGEN's Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers.

The results are displayed as easy-to-interpret PCR curves.

Applications

mericon Pathogen Detection Assays are for real-time PCR-based detection of specific bacterial pathogens in food, animal feed and pharmaceutical products.

Comparison of mericon Pathogen Detection Assays

Features Specifications
Quantitative/qualitative Qualitative (Quantitative for Quant L. pneumophila and Quant Legionella spp)
Recommended sample prep mericon DNA Bacteria Kit (Gram-negative bacteria) or mericon DNA Bacteria Plus Kit (Gram-positive bacteria) or QIAamp UCP Pathogen Mini Kit (Legionella-specific)
RUO/CE/ASR RUO
Sample type Bacterial preculture from food and water samples after concentration, e.g., filtration (for Legionella-specific Kits)
Thermal cycler Rotor-Gene Q and other cyclers
What detected DNA of respective pathogen