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Oligotex Direct mRNA Mini Kit

For mRNA purification from cells and tissues
  • High recovery of pure mRNA in as little as 30 minutes
  • No oligo-dT cellulose or ethanol precipitation
  • Flexibility for use with widely varying amounts of starting material
Oligotex Direct mRNA Kits enable purification of mRNA from cells and tissues. Each Oligotex Kit includes Oligotex resin for binding poly A+ mRNA, and spin columns for washing and eluting bound mRNA. For purification of mRNA from total RNA and cleanup of poly A+ in vitro transcripts, Oligotex mRNA Kits are available.

Specifications

Features
Specifications
Applications PCR, real-time PCR, microarray, transfection..
Elution volume 20–100 µl
Format Spin column
Main sample type Tissue, cells
Processing Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Poly A+ mRNA
Sample amount 100 mg/2 x 10e7
Stabilization Yes
Technology Polystyrene-latex particles
Time per run or per prep 30–50 minutes
Yield >90% recovery

Product Resources

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Kit Handbooks (1)
For Purification of poly A+ RNA from total RNA, purification of poly A+ RNA directly, from cultured cells or tissues, and purification of polyadenylated in vitro transcripts 
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User-Developed Protocols (2)
The procedure has been used successfully by customers for regeneration of Oligotex resin from spin columns following elution of poly A+ mRNA.
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The procedure has been successfully used for isolation of mRNA from adipose tissue by customers. Because of the nature of adipose tissue it cannot be used immediately in Oligotex Direct mRNA Protocols; simple centrifugation, however, eliminates the problem, and the procedure works fine.
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