Oligotex Direct mRNA Mini Kit

For mRNA purification from cells and tissues

High recovery of pure mRNA in as little as 30 minutes
No oligo-dT cellulose or ethanol precipitation
Flexibility for use with widely varying amounts of starting material

Oligotex Direct mRNA Kits enable purification of mRNA from cells and tissues. Each Oligotex Kit includes Oligotex resin for binding poly A⁺ mRNA, and spin columns for washing and eluting bound mRNA. For purification of mRNA from total RNA and cleanup of poly A⁺ in vitro transcripts, Oligotex mRNA Kits are available.

Specifications
Product Resources

Specifications

Features	Specifications
Applications	PCR, real-time PCR, microarray, transfection..
Elution volume	20–100 µl
Format	Spin column
Main sample type	Tissue, cells
Processing	Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Poly A+ mRNA
Sample amount	100 mg/2 x 10e7
Stabilization	Yes
Technology	Polystyrene-latex particles
Time per run or per prep	30–50 minutes
Yield	>90% recovery

Product Resources

FAQs (8)

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	Is mRNA isolation necessary for sensitive RT-PCR? FAQ ID -111	View
	How can I purify mRNA from total RNA using an Oligotex Direct mRNA Kit? FAQ ID -201	View
	What is the composition of buffer OW2? FAQ ID -419	View
	What is the composition of buffer OEB? FAQ ID -420	View
	What is the composition of buffer OCD? FAQ ID -423	View
	What is the composition of buffer OL2? FAQ ID -424	View
	What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer? FAQ ID -528	View
	How can I decrease ribosomal RNA (rRNA) contamination using Oligotex? FAQ ID -642	View

Kit Handbooks (1)

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	(EN) - Oligotex Handbook For Purification of poly A+ RNA from total RNA, purification of poly A+ RNA directly, from cultured cells or tissues, and purification of polyadenylated in vitro transcripts	Show details

Quick-Start Protocols (2)

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	Oligotex Direct mRNA Kit for animal tissues (EN)	Show details
	Oligotex Direct mRNA Kit for cultured cells (EN)	Show details

User-Developed Protocols (2)

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	Regeneration of Oligotex® resin from the Oligotex mRNA Kit - (EN) The procedure has been used successfully by customers for regeneration of Oligotex resin from spin columns following elution of poly A⁺mRNA.	Show details
	Isolation of mRNA from adipose tissue using Oligotex® Direct mRNA Kits - (EN) The procedure has been successfully used for isolation of mRNA from adipose tissue by customers. Because of the nature of adipose tissue it cannot be used immediately in Oligotex Direct mRNA Protocols; simple centrifugation, however, eliminates the problem, and the procedure works fine.	Show details

Safety Data Sheets (2)

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	MSDS Oligotex Direct mRNA Mini Kit (12)
	CofA