MagAttract Direct mRNA M48 Kit

Yields of mRNA using the MagAttract Direct mRNA M48 Kit are highly reproducible for replicate samples. Typically, mRNA isolation from 2 x 10⁶ BJAB cells gives a yield of 2 µg mRNA. Total yield from a sample is dependent on the sample type and condition and the number of actively transcribing cells in the sample.

The MagAttract Direct mRNA M48 Kit suitable for use with a wide range of cell types. The system combines automated purification of mRNA using proven MagAttract magnetic particle technology with the specificity of oligo-dT hybridization and scalable elution volumes, to deliver a highly efficient and cost-effective mRNA purification system for gene expression analysis projects.

Oligo-dT probes are covalently attached to the surface of MagAttract Direct mRNA particles in the presence of Buffer MRL. The mRNA bound to the magnetic particles is then efficiently washed with two different wash buffers to improve the purity of the mRNA. Highly pure mRNA is eluted in the elution buffer provided. Elution volumes may be from 50 µl to 100 µl. Samples are processed on the BioRobot M48 in 1.5 ml or 2 ml sample tubes. Up to 48 samples in increments of 6 samples can be processed per run. The sample source can be from 4 x 10⁵ up to 2 x 10⁶ cells and sample volume can vary from 150 µl to 740 µl.

The purified mRNA performs well in downstream assays, such as real-time RT-PCR on any real-time cycler, and microarray target preparation. The mRNA is also suitable for gene expression analysis applications, including:

• Northern blotting
• Melting curve analysis